Hybrid targeted and whole transcriptome amplification

ABSTRACT

Disclosed herein include systems, methods, compositions, and kits for the labeling of nucleic acids targets. In some embodiments, the methods comprise the use of target-specific primers and target-non-specific primers during library preparation (e.g., hybrid library preparation). In some embodiments, the methods increase the abundance of select low abundance targets during cDNA library preparation and/or sequencing library preparation.

RELATED APPLICATIONS

This application claims the benefit under 35 U.S.C. § 119(e) of U.S. Provisional Patent Application Ser. No. 62/805,956, filed Feb. 14, 2019, the content of this related application is incorporated herein by reference in its entirety for all purposes.

BACKGROUND Field

The present disclosure relates generally to the field of molecular biology, for example determining gene expression using molecular barcoding.

Description of the Related Art

Current technology allows measurement of gene expression of single cells in a massively parallel manner (e.g., >10000 cells) by attaching cell specific oligonucleotide barcodes to poly(A) mRNA molecules from individual cells as each of the cells is co-localized with a barcoded reagent bead in a compartment. However, the nucleic acids in a cell are often in a broad range of concentrations ranging several orders a magnitude. This greatly increases the amount of sequencing or screening necessary to fully assess a sample. There is a need for systems and methods of increasing the abundance of low abundance nucleic acids during library preparation.

SUMMARY

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides each comprising a first universal sequence and a barcode sequence; extending the plurality of oligonucleotides hybridized to the plurality of nucleic acid targets to generate a plurality of first strand barcoded polynucleotides; and synthesizing a plurality of second strand barcoded polynucleotides using the plurality of first strand barcoded polynucleotides as templates to generate a plurality of double-stranded barcoded polynucleotides. The method can comprise adding the sequence of an adaptor (e.g., ligating an adaptor) to the plurality of double-stranded barcoded polynucleotides, wherein the adaptor comprises a second universal sequence. The method can comprise amplifying the plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the second universal sequence, thereby generating a first plurality of barcoded amplicons comprising sequences of the nucleic acid targets, the first universal sequence, the second universal sequence, a complementary sequence thereof, and/or a portion thereof, and primers capable of hybridizing to two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, the first universal sequence, the second universal sequence, a complementary sequence thereof, and/or a portion thereof.

In some embodiments, the method comprises performing random priming and extension using random primers and the first plurality of barcoded amplicons and/or the second plurality of barcoded amplicons, or products thereof, as templates. In some embodiments, performing random priming and extension comprises adding sequences of sequencing adaptors to the first plurality of barcoded amplicons and the second plurality of barcoded amplicons, or products thereof. In some embodiments, the method comprises performing extension and/or amplification using target-specific primers and the first plurality of barcoded amplicons and/or the second plurality of barcoded amplicons, or products thereof, as templates. In some embodiments, performing extension and/or amplification comprises adding sequences of sequencing adaptors to the first plurality of barcoded amplicons and/or the second plurality of barcoded amplicons. In some embodiments, said sequencing adaptors comprises a binding site for a sequencing primer (e.g., Illumina Read 1 and Read 2 sequences) or a sequencing adapter (e.g., Illumina P5 and P7 sequences), a complementary sequence thereof, or a portion thereof.

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides each comprising a first universal sequence and a barcode sequence; extending the plurality of oligonucleotides hybridized to the plurality of nucleic acid targets to generate a plurality of first strand barcoded polynucleotides; and synthesizing a plurality of second strand barcoded polynucleotides using the plurality of first strand barcoded polynucleotides as templates to generate a plurality of double-stranded barcoded polynucleotides. The method can comprise adding the sequence of an adapter (e.g., by ligating the adaptor or PCR) to the plurality of double-stranded barcoded polynucleotides, wherein the adaptor comprises a second universal sequence. The method can comprise amplifying the plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the second universal sequence, thereby generating a first plurality of barcoded amplicons comprising sequences of the nucleic acid targets, the first universal sequence, the second universal sequence, complements thereof, and/or a portion thereof. The method can comprise performing extension and/or amplification using target-specific primers and the first plurality of barcoded amplicons, or products thereof, as templates.

In some embodiments, the method comprises performing random priming and extension using random primers and the first plurality of barcoded amplicons, or products thereof, as templates. In some embodiments, the method comprises amplifying the plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof. In some embodiments, performing random priming and extension comprises adding sequences of sequencing adaptors to the first plurality of barcoded amplicons and the second plurality of barcoded amplicons, or products thereof. In some embodiments, performing extension and/or amplification comprises adding sequences of sequencing adaptors to the first plurality of barcoded amplicons. In some embodiments, said sequencing adaptors comprises a sequencing primer binding site or a sequencing primer. In some embodiments, the sequencing primer is a Read 1 sequencing primer or a Read 2 sequencing primer.

In some embodiments, amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets comprises amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of amplifying the first universal sequence. In some embodiments, amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets comprises amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of amplifying the second universal sequence. In some embodiments, extending the plurality of oligonucleotides to generate the plurality of first strand barcoded polynucleotides comprises performing reverse transcription of one or more nucleic acid targets of the plurality of nucleic acid targets. In some embodiments, the plurality of first strand barcoded polynucleotides comprises barcoded single-stranded cDNA molecules. In some embodiments, extending the plurality of oligonucleotides to generate the plurality of first strand barcoded polynucleotides comprises performing nucleic acid extension of one or more oligonucleotides of the plurality of oligonucleotides hybridized to one or more nucleic acid targets of the plurality of nucleic acid targets. In some embodiments, the adaptor is ligated to 5′ end, 3′ end, or both of the plurality of double-stranded barcoded polynucleotides. In some embodiments, the method comprises obtaining sequence information of the first plurality of the barcoded amplicons and/or the second plurality of the barcoded amplicons, or products thereof.

In some embodiments, the first plurality of barcoded amplicons comprises whole transcriptome amplification (WTA) products. In some embodiments, the first plurality of barcoded amplicons are correspond to at least 10% of the mRNAs of a single cell. In some embodiments, the first plurality of barcoded amplicons are correspond to at least 50% of the mRNAs of a single cell. In some embodiments, the first plurality of barcoded amplicons are correspond to at least 90% of the mRNAs of a single cell. In some embodiments, the second plurality of barcoded amplicons correspond to at most 5% of the mRNAs of a single cell. In some embodiments, the second plurality of barcoded amplicons correspond to at most 10% of the mRNAs of a single cell. In some embodiments, the second plurality of barcoded amplicons correspond to at most 20% of the mRNAs of a single cell.

In some embodiments, the adaptor is a single-stranded polynucleotide. In some embodiments, the adaptor is a double-stranded polynucleotide. In some embodiments, the adaptor, or a strand of the adaptor, is from 2-30 nucleotides in length. In some embodiments, the first universal sequence and the second universal sequence are the same. In some embodiments, the first universal sequence and the second universal sequence are different. In some embodiments, each one of the plurality of amplicons comprises at least part of the first universal sequence, the second universal sequence, or both.

In some embodiments, adding the sequence of the adaptor to the plurality of double-stranded barcoded polynucleotides comprises ligating the adaptor to the plurality of double-stranded barcoded polynucleotides. Adding the sequence of the adaptor to the plurality of double-stranded barcoded polynucleotides can comprise adding the sequence of the adaptor to the plurality of double-stranded barcoded polynucleotides by nucleic acid extension or amplification. The amplification comprises polymerase chain reaction. Amplifying the plurality of double-stranded barcoded polynucleotides can comprise amplifying the plurality of double-stranded barcoded polynucleotides via linear amplification. The linear amplification can comprise 1-100 cycles of amplification. Amplifying the plurality of double-stranded barcoded polynucleotides can comprise amplifying the plurality of double-stranded barcoded polynucleotides via polymerase chain reaction (PCR). The PCR can comprise 1-100 cycles of PCR.

In some embodiments, performing the extension and/or amplification performing random performing 1-100 cycles of extension and/or amplification. The amplification can comprise polymerase chain reaction (PCR) amplification. In some embodiments, performing random priming, and extension and/or amplification, comprises performing 1-100 cycles of random priming, and extension and/or amplification. The amplification can comprise polymerase chain reaction (PCR) amplification.

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides; and extending the plurality of oligonucleotides in the presence of a reverse transcriptase and a template switch oligonucleotide (TSO) to generate a plurality of first strand barcoded polynucleotides comprising the TSO or a portion thereof, wherein each of the plurality of oligonucleotides and/or the template switch oligonucleotide comprises a first universal sequence and a barcode sequence. The method can comprise amplifying the plurality of first strand barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the TSO or the portion thereof to synthesize a first plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets, the first universal sequence, the TSO, or a portion thereof, and to generate a first plurality of double-stranded barcoded polynucleotides; and primers capable of hybridizing to two or more of the plurality of nucleic acid targets to synthesize a second plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets or a portion thereof, to generate a second plurality of double-stranded barcoded polynucleotides. The method can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and a sequence, or a subsequence, of the TSO, thereby generating a plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof. The method can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof.

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides; and extending the plurality of oligonucleotides in the presence of a reverse transcriptase and a template switch oligonucleotide (TSO) to generate a plurality of first strand barcoded polynucleotides comprising the TSO or a portion thereof, wherein each of the plurality of oligonucleotides and/or the template switch oligonucleotide comprises a first universal sequence and a barcode sequence. The method can comprise amplifying the plurality of first strand barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the TSO or the portion thereof to synthesize a first plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets, the first universal sequence, the TSO, or a portion thereof, and to generate a first plurality of double-stranded barcoded polynucleotides. The method can comprise amplifying the first plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and a sequence, or a subsequence, of the TSO, thereby generating a first plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof, and primers capable of hybridizing to the two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof.

In some embodiments, the method comprises amplifying the plurality of first strand barcoded polynucleotides using primers capable of hybridizing to two or more of the plurality of nucleic acid targets to synthesize a second plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets or a portion thereof, to generate a second plurality of double-stranded barcoded polynucleotides. In some embodiments, each of the plurality of oligonucleotides comprises a first universal sequence and a barcode sequence. In some embodiments, the template switch oligonucleotide comprises a second universal sequence. In some embodiments, the template switch oligonucleotide comprises a first universal sequence and a barcode sequence. In some embodiments, each of the plurality of oligonucleotides comprises a second universal sequence. In some embodiments, the method comprises amplifying the first plurality of second strand barcoded polynucleotides and/or the second plurality of second strand barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets. In some embodiments, amplifying the first plurality of second strand barcoded polynucleotides and/or the second plurality of second strand barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of hybridizing to the first universal sequence. In some embodiments, amplifying the first plurality of second strand barcoded polynucleotides and/or the second plurality of second strand barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of hybridizing to the sequence, or a subsequence, of the TSO.

In some embodiments, amplifying the plurality of first stranded barcoded polynucleotides comprises amplifying the plurality of first stranded barcoded polynucleotides comprises 1-100 cycles. Amplifying the plurality of first stranded barcoded polynucleotides can comprise performing linear amplification of the plurality of first stranded barcoded polynucleotides. Amplifying the plurality of first stranded barcoded polynucleotides can comprise performing polymerase chain reaction (PCR) amplification of the plurality of first stranded barcoded polynucleotides. Amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides 1-100 cycles. Amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides via linear amplification. Amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides via polymerase chain reaction (PCR) amplification.

In some embodiments, the method comprises dividing the first plurality of second strand barcoded polynucleotides and the second plurality of second strand barcoded polynucleotides into two pools, wherein amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides using (1) the primers capable of hybridizing to the first universal sequence and a sequence, or a subsequence, of the TSO is performed in one reaction using one of the two pools and (2) the primers capable of hybridizing to two or more of the plurality of nucleic acid targets is performed in another reaction using the other of the two pools. In some embodiments, the method comprises obtaining sequence information of the first plurality of barcoded amplicons and/or second plurality of barcoded amplicons, or products thereof.

In some embodiments, the method comprises performing extension and/or amplification using target-specific primer and the first plurality of barcoded amplicons and/or second plurality of barcoded amplicons as templates. In some embodiments, extending the plurality of oligonucleotides to generate the plurality of first strand barcoded polynucleotides comprises performing reverse transcription of one or more nucleic acid targets of the plurality of nucleic acid targets to generate barcoded single-stranded cDNA molecules. In some embodiments, extending the plurality of oligonucleotides to generate the plurality of first strand barcoded polynucleotides comprises performing nucleic acid extension of oligonucleotides of the plurality of oligonucleotides hybridized to one or more nucleic acid targets of the plurality of nucleic acid targets to generate barcoded single-stranded nucleic acid molecules. In some embodiments, the reverse transcriptase comprises a viral reverse transcriptase. In some embodiments, the viral reverse transcriptase is a murine leukemia virus (MMLV) reverse transcriptase. In some embodiments, the viral reverse transcriptase is a Moloney murine leukemia virus (MMLV) reverse transcriptase.

In some embodiments, the two or more of the plurality of nucleic acid targets comprises mRNAs of a low-expressing gene.

The total number of occurrences of the two or more of the plurality of nucleic acid targets can comprise mRNAs of at most 1% of the mRNAs of a single cell. The total number of occurrences of the two or more of the plurality of nucleic acid targets can comprise mRNAs of at most 5% of the mRNAs of a single cell. The total number of occurrences of the two or more of the plurality of nucleic acid targets can comprise mRNAs of at most 10% of the mRNAs of a single cell.

In some embodiments, said barcode sequence comprises a molecular label, a cellular label, and wherein said oligonucleotide comprises a target-specific region, or any combination thereof. In some embodiments, said target-specific region comprises an oligo dT sequence, a random sequence, a target-specific sequence, or a combination thereof. In some embodiments, the plurality of nucleic acid targets comprises DNAs, mRNAs, or a combination thereof. In some embodiments, the plurality of nucleic acid targets are mRNAs. In some embodiments, each of the plurality of oligonucleotides comprising the first universal sequence and the barcode sequence is immobilized on a solid support. In some embodiments, the solid support is a bead. In some embodiments, at least two of the plurality of oligonucleotides immobilized on a single solid support comprises different molecular labels. In some embodiments, said plurality of nucleic acids attached to a solid support comprises the same cellular label. In some embodiments, the plurality of nucleic acid targets are nucleic acids from a single sample. In some embodiments, the sample is a single cell. In some embodiments, the sample comprises a plurality of cells. In some embodiments, the single cell is or the plurality of cells comprises a brain cell, a heart cell, a cancer cell, a circulating tumor cell, an organ cell, an epithelial cell, a metastatic cell, a benign cell, a primary cell, a circulatory cell, or a combination thereof. In some embodiments, hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides occurs in a partition. In some embodiments, the partition is a microwell or a droplet. In some embodiments, the method comprises lysing a single cell to release the plurality of nucleic acid targets. In some embodiments, each of the barcoded amplicons comprises a barcode sequence. In some embodiments, said barcode sequence comprises a molecular label, a cellular label or a combination thereof. In some embodiments, the method comprises sequencing the barcoded amplicons to generate a plurality of sequencing reads comprising a molecular label, a cellular label, or any combination thereof. In some embodiments, the method comprises analyzing the plurality of sequencing reads using the cellular label. In some embodiments, the method comprises analyzing the plurality of sequencing reads using the molecular label.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 illustrates a non-limiting exemplary barcode.

FIG. 2 shows a non-limiting exemplary workflow of barcoding and digital counting.

FIG. 3 is a schematic illustration showing a non-limiting exemplary process for generating an indexed library of targets barcoded at the 3′-ends from a plurality of targets.

FIG. 4 is a schematic illustration of a non-limiting exemplary workflow of performing whole transcriptome analysis (WTA).

FIG. 5 is a schematic illustration of a non-limiting exemplary workflow of determining the expression profile of a panel of target genes using a panel of target-specific multiplex primers.

FIG. 6 is a schematic illustration of a non-limiting exemplary workflow of performing hybrid cDNA library preparation followed by non-hybrid sequencing library preparation.

FIG. 7 is a schematic illustration of a non-limiting exemplary workflow of performing non-hybrid cDNA library preparation followed by hybrid sequencing library preparation.

FIG. 8 is a schematic illustration of a non-limiting exemplary workflows for alternative single-cell RNA sequencing (scRNA-seq) embodiments comprising the methods of hybrid cDNA library preparation and/or hybrid sequencing library preparation as disclosed herein.

FIG. 9 is a schematic illustration of a non-limiting exemplary workflow of performing hybrid cDNA library preparation followed by hybrid sequencing library preparation.

FIG. 10 is a schematic illustration of a non-limiting exemplary workflow of performing non-hybrid cDNA library preparation followed by splitting the amplicons into pools for separately conducing non-hybrid sequencing library preparation and hybrid sequencing library preparation.

DETAILED DESCRIPTION

In the following detailed description, reference is made to the accompanying drawings, which form a part hereof. In the drawings, similar symbols typically identify similar components, unless context dictates otherwise. The illustrative embodiments described in the detailed description, drawings, and claims are not meant to be limiting. Other embodiments may be utilized, and other changes may be made, without departing from the spirit or scope of the subject matter presented herein. It will be readily understood that the aspects of the present disclosure, as generally described herein, and illustrated in the Figures, can be arranged, substituted, combined, separated, and designed in a wide variety of different configurations, all of which are explicitly contemplated herein and made part of the disclosure herein.

All patents, published patent applications, other publications, and sequences from GenBank, and other databases referred to herein are incorporated by reference in their entirety with respect to the related technology.

Quantifying small numbers of nucleic acids, for example messenger ribonucleotide acid (mRNA) molecules, is clinically important for determining, for example, the genes that are expressed in a cell at different stages of development or under different environmental conditions. However, it can also be very challenging to determine the absolute number of nucleic acid molecules (e.g., mRNA molecules), especially when the number of molecules is very small. One method to determine the absolute number of molecules in a sample is digital polymerase chain reaction (PCR). Ideally, PCR produces an identical copy of a molecule at each cycle. However, PCR can have disadvantages such that each molecule replicates with a stochastic probability, and this probability varies by PCR cycle and gene sequence, resulting in amplification bias and inaccurate gene expression measurements. Stochastic barcodes with unique molecular labels (also referred to as molecular indexes (MIs)) can be used to count the number of molecules and correct for amplification bias. Stochastic barcoding, such as the Precise™ assay (Cellular Research, Inc. (Palo Alto, Calif.)) and Rhapsody™ assay (Becton, Dickinson and Company (Franklin Lakes, N.J.)), can correct for bias induced by PCR and library preparation steps by using molecular labels (MLs) to label mRNAs during reverse transcription (RT).

The Precise™ assay can utilize a non-depleting pool of stochastic barcodes with large number, for example 6561 to 65536, unique molecular label sequences on poly(T) oligonucleotides to hybridize to all poly(A)-mRNAs in a sample during the RT step. A stochastic barcode can comprise a universal PCR priming site. During RT, target gene molecules react randomly with stochastic barcodes. Each target molecule can hybridize to a stochastic barcode resulting to generate stochastically barcoded complementary ribonucleotide acid (cDNA) molecules). After labeling, stochastically barcoded cDNA molecules from microwells of a microwell plate can be pooled into a single tube for PCR amplification and sequencing. Raw sequencing data can be analyzed to produce the number of reads, the number of stochastic barcodes with unique molecular label sequences, and the numbers of mRNA molecules.

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides each comprising a first universal sequence and a barcode sequence; extending the plurality of oligonucleotides hybridized to the plurality of nucleic acid targets to generate a plurality of first strand barcoded polynucleotides; and synthesizing a plurality of second strand barcoded polynucleotides using the plurality of first strand barcoded polynucleotides as templates to generate a plurality of double-stranded barcoded polynucleotides. The method can comprise adding the sequence of an adaptor (e.g., ligating an adaptor) to the plurality of double-stranded barcoded polynucleotides, wherein the adaptor comprises a second universal sequence. The method can comprise amplifying the plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the second universal sequence, thereby generating a first plurality of barcoded amplicons comprising sequences of the nucleic acid targets, the first universal sequence, the second universal sequence, a complementary sequence thereof, and/or a portion thereof, and primers capable of hybridizing to two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof.

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides each comprising a first universal sequence and a barcode sequence; extending the plurality of oligonucleotides hybridized to the plurality of nucleic acid targets to generate a plurality of first strand barcoded polynucleotides; and synthesizing a plurality of second strand barcoded polynucleotides using the plurality of first strand barcoded polynucleotides as templates to generate a plurality of double-stranded barcoded polynucleotides. The method can comprise adding the sequence of an adaptor (e.g., ligating an adaptor) to the plurality of double-stranded barcoded polynucleotides, wherein the adaptor comprises a second universal sequence. The method can comprise amplifying the plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the second universal sequence, thereby generating a first plurality of barcoded amplicons comprising sequences of the nucleic acid targets, the first universal sequence, the second universal sequence, complements thereof, and/or a portion thereof. The method can comprise performing extension and/or amplification using target-specific primers and the first plurality of barcoded amplicons, or products thereof, as templates.

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides; and extending the plurality of oligonucleotides in the presence of a reverse transcriptase and a template switch oligonucleotide (TSO) to generate a plurality of first strand barcoded polynucleotides comprising the TSO or a portion thereof, wherein each of the plurality of oligonucleotides and/or the template switch oligonucleotide comprises a first universal sequence and a barcode sequence. The method can comprise amplifying the plurality of first strand barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the TSO or the portion thereof to synthesize a first plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets, the first universal sequence, the TSO, or a portion thereof, and to generate a first plurality of double-stranded barcoded polynucleotides; and primers capable of hybridizing to two or more of the plurality of nucleic acid targets to synthesize a second plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets or a portion thereof, to generate a second plurality of double-stranded barcoded polynucleotides. The method can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and a sequence, or a subsequence, of the TSO, thereby generating a first plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof.

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides; and extending the plurality of oligonucleotides in the presence of a reverse transcriptase and a template switch oligonucleotide (TSO) to generate a plurality of first strand barcoded polynucleotides comprising the TSO or a portion thereof, wherein each of the plurality of oligonucleotides and/or the template switch oligonucleotide comprises a first universal sequence and a barcode sequence. The method can comprise amplifying the plurality of first strand barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the TSO or the portion thereof to synthesize a first plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets, the first universal sequence, the TSO, or a portion thereof, and to generate a first plurality of double-stranded barcoded polynucleotides. The method can comprise amplifying the first plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and a sequence, or a subsequence, of the TSO, thereby generating a first plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof, and primers capable of hybridizing to the two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof.

Definitions

Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present disclosure belongs. See, e.g., Singleton et al., Dictionary of Microbiology and Molecular Biology 2nd ed., J. Wiley & Sons (New York, N.Y. 1994); Sambrook et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press (Cold Spring Harbor, N.Y. 1989). For purposes of the present disclosure, the following terms are defined below.

As used herein, the term “adaptor” can mean a sequence to facilitate amplification or sequencing of associated nucleic acids. The associated nucleic acids can comprise target nucleic acids. The associated nucleic acids can comprise one or more of spatial labels, target labels, sample labels, indexing label, or barcode sequences (e.g., molecular labels). The adaptors can be linear. The adaptors can be pre-adenylated adaptors. The adaptors can be double- or single-stranded. One or more adaptor can be located on the 5′ or 3′ end of a nucleic acid. When the adaptors comprise known sequences on the 5′ and 3′ ends, the known sequences can be the same or different sequences. An adaptor located on the 5′ and/or 3′ ends of a polynucleotide can be capable of hybridizing to one or more oligonucleotides immobilized on a surface. An adaptor can, in some embodiments, comprise a universal sequence. A universal sequence can be a region of nucleotide sequence that is common to two or more nucleic acid molecules. The two or more nucleic acid molecules can also have regions of different sequence. Thus, for example, the 5′ adaptors can comprise identical and/or universal nucleic acid sequences and the 3′ adaptors can comprise identical and/or universal sequences. A universal sequence that may be present in different members of a plurality of nucleic acid molecules can allow the replication or amplification of multiple different sequences using a single universal primer that is complementary to the universal sequence. Similarly, at least one, two (e.g., a pair) or more universal sequences that may be present in different members of a collection of nucleic acid molecules can allow the replication or amplification of multiple different sequences using at least one, two (e.g., a pair) or more single universal primers that are complementary to the universal sequences. Thus, a universal primer includes a sequence that can hybridize to such a universal sequence. The target nucleic acid sequence-bearing molecules may be modified to attach universal adaptors (e.g., non-target nucleic acid sequences) to one or both ends of the different target nucleic acid sequences. The one or more universal primers attached to the target nucleic acid can provide sites for hybridization of universal primers. The one or more universal primers attached to the target nucleic acid can be the same or different from each other.

As used herein the term “associated” or “associated with” can mean that two or more species are identifiable as being co-located at a point in time. An association can mean that two or more species are or were within a similar container. An association can be an informatics association. For example, digital information regarding two or more species can be stored and can be used to determine that one or more of the species were co-located at a point in time. An association can also be a physical association. In some embodiments, two or more associated species are “tethered”, “attached”, or “immobilized” to one another or to a common solid or semisolid surface. An association may refer to covalent or non-covalent means for attaching labels to solid or semi-solid supports such as beads. An association may be a covalent bond between a target and a label. An association can comprise hybridization between two molecules (such as a target molecule and a label).

As used herein, the term “complementary” can refer to the capacity for precise pairing between two nucleotides. For example, if a nucleotide at a given position of a nucleic acid is capable of hydrogen bonding with a nucleotide of another nucleic acid, then the two nucleic acids are considered to be complementary to one another at that position. Complementarity between two single-stranded nucleic acid molecules may be “partial,” in which only some of the nucleotides bind, or it may be complete when total complementarity exists between the single-stranded molecules. A first nucleotide sequence can be said to be the “complement” of a second sequence if the first nucleotide sequence is complementary to the second nucleotide sequence. A first nucleotide sequence can be said to be the “reverse complement” of a second sequence, if the first nucleotide sequence is complementary to a sequence that is the reverse (i.e., the order of the nucleotides is reversed) of the second sequence. As used herein, a “complementary” sequence can refer to a “complement” or a “reverse complement” of a sequence. It is understood from the disclosure that if a molecule can hybridize to another molecule it may be complementary, or partially complementary, to the molecule that is hybridizing.

As used herein, the term “digital counting” can refer to a method for estimating a number of target molecules in a sample. Digital counting can include the step of determining a number of unique labels that have been associated with targets in a sample. This methodology, which can be stochastic in nature, transforms the problem of counting molecules from one of locating and identifying identical molecules to a series of yes/no digital questions regarding detection of a set of predefined labels.

As used herein, the term “label” or “labels” can refer to nucleic acid codes associated with a target within a sample. A label can be, for example, a nucleic acid label. A label can be an entirely or partially amplifiable label. A label can be entirely or partially sequencable label. A label can be a portion of a native nucleic acid that is identifiable as distinct. A label can be a known sequence. A label can comprise a junction of nucleic acid sequences, for example a junction of a native and non-native sequence. As used herein, the term “label” can be used interchangeably with the terms, “index”, “tag,” or “label-tag.” Labels can convey information. For example, in various embodiments, labels can be used to determine an identity of a sample, a source of a sample, an identity of a cell, and/or a target.

As used herein, the term “non-depleting reservoirs” can refer to a pool of barcodes (e.g., stochastic barcodes) made up of many different labels. A non-depleting reservoir can comprise large numbers of different barcodes such that when the non-depleting reservoir is associated with a pool of targets each target is likely to be associated with a unique barcode. The uniqueness of each labeled target molecule can be determined by the statistics of random choice, and depends on the number of copies of identical target molecules in the collection compared to the diversity of labels. The size of the resulting set of labeled target molecules can be determined by the stochastic nature of the barcoding process, and analysis of the number of barcodes detected then allows calculation of the number of target molecules present in the original collection or sample. When the ratio of the number of copies of a target molecule present to the number of unique barcodes is low, the labeled target molecules are highly unique (i.e., there is a very low probability that more than one target molecule will have been labeled with a given label).

As used herein, the term “nucleic acid” refers to a polynucleotide sequence, or fragment thereof. A nucleic acid can comprise nucleotides. A nucleic acid can be exogenous or endogenous to a cell. A nucleic acid can exist in a cell-free environment. A nucleic acid can be a gene or fragment thereof. A nucleic acid can be DNA. A nucleic acid can be RNA. A nucleic acid can comprise one or more analogs (e.g., altered backbone, sugar, or nucleobase). Some non-limiting examples of analogs include: 5-bromouracil, peptide nucleic acid, xeno nucleic acid, morpholinos, locked nucleic acids, glycol nucleic acids, threose nucleic acids, dideoxynucleotides, cordycepin, 7-deaza-GTP, fluorophores (e.g., rhodamine or fluorescein linked to the sugar), thiol containing nucleotides, biotin linked nucleotides, fluorescent base analogs, CpG islands, methyl-7-guanosine, methylated nucleotides, inosine, thiouridine, pseudouridine, dihydrouridine, queuosine, and wyosine. “Nucleic acid”, “polynucleotide, “target polynucleotide”, and “target nucleic acid” can be used interchangeably.

A nucleic acid can comprise one or more modifications (e.g., a base modification, a backbone modification), to provide the nucleic acid with a new or enhanced feature (e.g., improved stability). A nucleic acid can comprise a nucleic acid affinity tag. A nucleoside can be a base-sugar combination. The base portion of the nucleoside can be a heterocyclic base. The two most common classes of such heterocyclic bases are the purines and the pyrimidines. Nucleotides can be nucleosides that further include a phosphate group covalently linked to the sugar portion of the nucleoside. For those nucleosides that include a pentofuranosyl sugar, the phosphate group can be linked to the 2′, the 3′, or the 5′ hydroxyl moiety of the sugar. In forming nucleic acids, the phosphate groups can covalently link adjacent nucleosides to one another to form a linear polymeric compound. In turn, the respective ends of this linear polymeric compound can be further joined to form a circular compound; however, linear compounds are generally suitable. In addition, linear compounds may have internal nucleotide base complementarity and may therefore fold in a manner as to produce a fully or partially double-stranded compound. Within nucleic acids, the phosphate groups can commonly be referred to as forming the internucleoside backbone of the nucleic acid. The linkage or backbone can be a 3′ to 5′ phosphodiester linkage.

A nucleic acid can comprise a modified backbone and/or modified internucleoside linkages. Modified backbones can include those that retain a phosphorus atom in the backbone and those that do not have a phosphorus atom in the backbone. Suitable modified nucleic acid backbones containing a phosphorus atom therein can include, for example, phosphorothioates, chiral phosphorothioates, phosphorodithioates, phosphotriesters, aminoalkyl phosphotriesters, methyl and other alkyl phosphonate such as 3′-alkylene phosphonates, 5′-alkylene phosphonates, chiral phosphonates, phosphinates, phosphoramidates including 3′-amino phosphoramidate and aminoalkyl phosphoramidates, phosphorodiamidates, thionophosphoramidates, thionoalkylphosphonates, thionoalkylphosphotriesters, selenophosphates, and boranophosphates having normal 3′-5′ linkages, 2′-5′ linked analogs, and those having inverted polarity wherein one or more internucleotide linkages is a 3′ to 3′, a 5′ to 5′ or a 2′ to 2′ linkage.

A nucleic acid can comprise polynucleotide backbones that are formed by short chain alkyl or cycloalkyl internucleoside linkages, mixed heteroatom and alkyl or cycloalkyl internucleoside linkages, or one or more short chain heteroatomic or heterocyclic internucleoside linkages. These can include those having morpholino linkages (formed in part from the sugar portion of a nucleoside); siloxane backbones; sulfide, sulfoxide and sulfone backbones; formacetyl and thioformacetyl backbones; methylene formacetyl and thioformacetyl backbones; riboacetyl backbones; alkene containing backbones; sulfamate backbones; methyleneimino and methylenehydrazino backbones; sulfonate and sulfonamide backbones; amide backbones; and others having mixed N, O, S and CH₂ component parts.

A nucleic acid can comprise a nucleic acid mimetic. The term “mimetic” can be intended to include polynucleotides wherein only the furanose ring or both the furanose ring and the internucleotide linkage are replaced with non-furanose groups, replacement of only the furanose ring can also be referred as being a sugar surrogate. The heterocyclic base moiety or a modified heterocyclic base moiety can be maintained for hybridization with an appropriate target nucleic acid. One such nucleic acid can be a peptide nucleic acid (PNA). In a PNA, the sugar-backbone of a polynucleotide can be replaced with an amide containing backbone, in particular an aminoethylglycine backbone. The nucleotides can be retained and are bound directly or indirectly to aza nitrogen atoms of the amide portion of the backbone. The backbone in PNA compounds can comprise two or more linked aminoethylglycine units which gives PNA an amide containing backbone. The heterocyclic base moieties can be bound directly or indirectly to aza nitrogen atoms of the amide portion of the backbone.

A nucleic acid can comprise a morpholino backbone structure. For example, a nucleic acid can comprise a 6-membered morpholino ring in place of a ribose ring. In some of these embodiments, a phosphorodiamidate or other non-phosphodiester internucleoside linkage can replace a phosphodiester linkage.

A nucleic acid can comprise linked morpholino units (e.g., morpholino nucleic acid) having heterocyclic bases attached to the morpholino ring. Linking groups can link the morpholino monomeric units in a morpholino nucleic acid. Non-ionic morpholino-based oligomeric compounds can have less undesired interactions with cellular proteins. Morpholino-based polynucleotides can be nonionic mimics of nucleic acids. A variety of compounds within the morpholino class can be joined using different linking groups. A further class of polynucleotide mimetic can be referred to as cyclohexenyl nucleic acids (CeNA). The furanose ring normally present in a nucleic acid molecule can be replaced with a cyclohexenyl ring. CeNA DMT protected phosphoramidite monomers can be prepared and used for oligomeric compound synthesis using phosphoramidite chemistry. The incorporation of CeNA monomers into a nucleic acid chain can increase the stability of a DNA/RNA hybrid. CeNA oligoadenylates can form complexes with nucleic acid complements with similar stability to the native complexes. A further modification can include Locked Nucleic Acids (LNAs) in which the 2′-hydroxyl group is linked to the 4′ carbon atom of the sugar ring thereby forming a 2′-C, 4′-C-oxymethylene linkage thereby forming a bicyclic sugar moiety. The linkage can be a methylene (—CH₂), group bridging the 2′ oxygen atom and the 4′ carbon atom wherein n is 1 or 2. LNA and LNA analogs can display very high duplex thermal stabilities with complementary nucleic acid (Tm=+3 to +10° C.), stability towards 3′-exonucleolytic degradation and good solubility properties.

A nucleic acid may also include nucleobase (often referred to simply as “base”) modifications or substitutions. As used herein, “unmodified” or “natural” nucleobases can include the purine bases, (e.g., adenine (A) and guanine (G)), and the pyrimidine bases, (e.g., thymine (T), cytosine (C) and uracil (U)). Modified nucleobases can include other synthetic and natural nucleobases such as 5-methylcytosine (5-me-C), 5-hydroxymethyl cytosine, xanthine, hypoxanthine, 2-aminoadenine, 6-methyl and other alkyl derivatives of adenine and guanine, 2-propyl and other alkyl derivatives of adenine and guanine, 2-thiouracil, 2-thiothymine and 2-thiocytosine, 5-halouracil and cytosine, 5-propynyl (—C═C—CH3) uracil and cytosine and other alkynyl derivatives of pyrimidine bases, 6-azo uracil, cytosine and thymine, 5-uracil (pseudouracil), 4-thiouracil, 8-halo, 8-amino, 8-thiol, 8-thioalkyl, 8-hydroxyl and other 8-substituted adenines and guanines, 5-halo particularly 5-bromo, 5-trifluoromethyl and other 5-substituted uracils and cytosines, 7-methylguanine and 7-methyladenine, 2-F-adenine, 2-aminoadenine, 8-azaguanine and 8-azaadenine, 7-deazaguanine and 7-deazaadenine and 3-deazaguanine and 3-deazaadenine. Modified nucleobases can include tricyclic pyrimidines such as phenoxazine cytidine(1H-pyrimido(5,4-b)(1,4)benzoxazin-2(3H)-one), phenothiazine cytidine (1H-pyrimido(5,4-b)(1,4)benzothiazin-2(3H)-one), G-clamps such as a substituted phenoxazine cytidine (e.g., 9-(2-aminoethoxy)-H-pyrimido(5,4-(b) (1,4)benzoxazin-2(3H)-one), phenothiazine cytidine (1H-pyrimido(5,4-b)(1,4)benzothiazin-2(3H)-one), G-clamps such as a substituted phenoxazine cytidine (e.g., 9-(2-aminoethoxy)-H-pyrimido(5,4-(b) (1,4)benzoxazin-2(3H)-one), carbazole cytidine (2H-pyrimido(4,5-b)indol-2-one), pyridoindole cytidine (H-pyrido(3′,2′:4,5)pyrrolo[2,3-d]pyrimidin-2-one).

As used herein, the term “sample” can refer to a composition comprising targets. Suitable samples for analysis by the disclosed methods, devices, and systems include cells, tissues, organs, or organisms.

As used herein, the term “sampling device” or “device” can refer to a device which may take a section of a sample and/or place the section on a substrate. A sample device can refer to, for example, a fluorescence activated cell sorting (FACS) machine, a cell sorter machine, a biopsy needle, a biopsy device, a tissue sectioning device, a microfluidic device, a blade grid, and/or a microtome.

As used herein, the term “solid support” can refer to discrete solid or semi-solid surfaces to which a plurality of barcodes (e.g., stochastic barcodes) may be attached. A solid support may encompass any type of solid, porous, or hollow sphere, ball, bearing, cylinder, or other similar configuration composed of plastic, ceramic, metal, or polymeric material (e.g., hydrogel) onto which a nucleic acid may be immobilized (e.g., covalently or non-covalently). A solid support may comprise a discrete particle that may be spherical (e.g., microspheres) or have a non-spherical or irregular shape, such as cubic, cuboid, pyramidal, cylindrical, conical, oblong, or disc-shaped, and the like. A bead can be non-spherical in shape. A plurality of solid supports spaced in an array may not comprise a substrate. A solid support may be used interchangeably with the term “bead.”

As used herein, the term “stochastic barcode” can refer to a polynucleotide sequence comprising labels of the present disclosure. A stochastic barcode can be a polynucleotide sequence that can be used for stochastic barcoding. Stochastic barcodes can be used to quantify targets within a sample. Stochastic barcodes can be used to control for errors which may occur after a label is associated with a target. For example, a stochastic barcode can be used to assess amplification or sequencing errors. A stochastic barcode associated with a target can be called a stochastic barcode-target or stochastic barcode-tag-target.

As used herein, the term “gene-specific stochastic barcode” can refer to a polynucleotide sequence comprising labels and a target-binding region that is gene-specific. A stochastic barcode can be a polynucleotide sequence that can be used for stochastic barcoding. Stochastic barcodes can be used to quantify targets within a sample. Stochastic barcodes can be used to control for errors which may occur after a label is associated with a target. For example, a stochastic barcode can be used to assess amplification or sequencing errors. A stochastic barcode associated with a target can be called a stochastic barcode-target or stochastic barcode-tag-target.

As used herein, the term “stochastic barcoding” can refer to the random labeling (e.g., barcoding) of nucleic acids. Stochastic barcoding can utilize a recursive Poisson strategy to associate and quantify labels associated with targets. As used herein, the term “stochastic barcoding” can be used interchangeably with “stochastic labeling.”

As used here, the term “target” can refer to a composition which can be associated with a barcode (e.g., a stochastic barcode). Exemplary suitable targets for analysis by the disclosed methods, devices, and systems include oligonucleotides, DNA, RNA, mRNA, microRNA, tRNA, and the like. Targets can be single or double stranded. In some embodiments, targets can be proteins, peptides, or polypeptides. In some embodiments, targets are lipids. As used herein, “target” can be used interchangeably with “species.”

As used herein, the term “reverse transcriptases” can refer to a group of enzymes having reverse transcriptase activity (i.e., that catalyze synthesis of DNA from an RNA template). In general, such enzymes include, but are not limited to, retroviral reverse transcriptase, retrotransposon reverse transcriptase, retroplasmid reverse transcriptases, retron reverse transcriptases, bacterial reverse transcriptases, group II intron-derived reverse transcriptase, and mutants, variants or derivatives thereof. Non-retroviral reverse transcriptases include non-LTR retrotransposon reverse transcriptases, retroplasmid reverse transcriptases, retron reverse transcriptases, and group II intron reverse transcriptases. Examples of group II intron reverse transcriptases include the Lactococcus lactis LI.LtrB intron reverse transcriptase, the Thermosynechococcus elongatus TeI4c intron reverse transcriptase, or the Geobacillus stearothermophilus GsI-IIC intron reverse transcriptase. Other classes of reverse transcriptases can include many classes of non-retroviral reverse transcriptases (i.e., retrons, group II introns, and diversity-generating retroelements among others).

The terms “universal adaptor primer,” “universal primer adaptor” or “universal adaptor sequence” are used interchangeably to refer to a nucleotide sequence that can be used to hybridize to barcodes (e.g., stochastic barcodes) to generate gene-specific barcodes. A universal adaptor sequence can, for example, be a known sequence that is universal across all barcodes used in methods of the disclosure. For example, when multiple targets are being labeled using the methods disclosed herein, each of the target-specific sequences may be linked to the same universal adaptor sequence. In some embodiments, more than one universal adaptor sequences may be used in the methods disclosed herein. For example, when multiple targets are being labeled using the methods disclosed herein, at least two of the target-specific sequences are linked to different universal adaptor sequences. A universal adaptor primer and its complement may be included in two oligonucleotides, one of which comprises a target-specific sequence and the other comprises a barcode. For example, a universal adaptor sequence may be part of an oligonucleotide comprising a target-specific sequence to generate a nucleotide sequence that is complementary to a target nucleic acid. A second oligonucleotide comprising a barcode and a complementary sequence of the universal adaptor sequence may hybridize with the nucleotide sequence and generate a target-specific barcode (e.g., a target-specific stochastic barcode). In some embodiments, a universal adaptor primer has a sequence that is different from a universal PCR primer used in the methods of this disclosure.

Barcodes

Barcoding, such as stochastic barcoding, has been described in, for example, Fu et al., Proc Natl Acad Sci U.S.A., 2011 May 31, 108(22):9026-31; U.S. Patent Application Publication No. US2011/0160078; Fan et al., Science, 2015 Feb. 6, 347(6222):1258367; US Patent Application Publication No. US2015/0299784; and PCT Application Publication No. WO2015/031691; the content of each of these, including any supporting or supplemental information or material, is incorporated herein by reference in its entirety. In some embodiments, the barcode disclosed herein can be a stochastic barcode which can be a polynucleotide sequence that may be used to stochastically label (e.g., barcode, tag) a target. Barcodes can be referred to stochastic barcodes if the ratio of the number of different barcode sequences of the stochastic barcodes and the number of occurrence of any of the targets to be labeled can be, or be about, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 30:1, 40:1, 50:1, 60:1, 70:1, 80:1, 90:1, 100:1, or a number or a range between any two of these values. A target can be an mRNA species comprising mRNA molecules with identical or nearly identical sequences. Barcodes can be referred to as stochastic barcodes if the ratio of the number of different barcode sequences of the stochastic barcodes and the number of occurrence of any of the targets to be labeled is at least, or is at most, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 30:1, 40:1, 50:1, 60:1, 70:1, 80:1, 90:1, or 100:1. Barcode sequences of stochastic barcodes can be referred to as molecular labels.

A barcode, for example a stochastic barcode, can comprise one or more labels. Exemplary labels can include a universal label, a cell label, a barcode sequence (e.g., a molecular label), a sample label, a plate label, a spatial label, and/or a pre-spatial label. FIG. 1 illustrates an exemplary barcode 104 with a spatial label. The barcode 104 can comprise a 5′amine that may link the barcode to a solid support 105. The barcode can comprise a universal label, a dimension label, a spatial label, a cell label, and/or a molecular label. The order of different labels (including but not limited to the universal label, the dimension label, the spatial label, the cell label, and the molecule label) in the barcode can vary. For example, as shown in FIG. 1, the universal label may be the 5′-most label, and the molecular label may be the 3′-most label. The spatial label, dimension label, and the cell label may be in any order. In some embodiments, the universal label, the spatial label, the dimension label, the cell label, and the molecular label are in any order. The barcode can comprise a target-binding region. The target-binding region can interact with a target (e.g., target nucleic acid, RNA, mRNA, DNA) in a sample. For example, a target-binding region can comprise an oligo(dT) sequence which can interact with poly(A) tails of mRNAs. In some instances, the labels of the barcode (e.g., universal label, dimension label, spatial label, cell label, and barcode sequence) may be separated by 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 or more nucleotides.

A label, for example the cell label, can comprise a unique set of nucleic acid sub-sequences of defined length, e.g., seven nucleotides each (equivalent to the number of bits used in some Hamming error correction codes), which can be designed to provide error correction capability. The set of error correction sub-sequences comprise seven nucleotide sequences can be designed such that any pairwise combination of sequences in the set exhibits a defined “genetic distance” (or number of mismatched bases), for example, a set of error correction sub-sequences can be designed to exhibit a genetic distance of three nucleotides. In this case, review of the error correction sequences in the set of sequence data for labeled target nucleic acid molecules (described more fully below) can allow one to detect or correct amplification or sequencing errors. In some embodiments, the length of the nucleic acid sub-sequences used for creating error correction codes can vary, for example, they can be, or be about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 30, 31, 40, 50, or a number or a range between any two of these values, nucleotides in length. In some embodiments, nucleic acid sub-sequences of other lengths can be used for creating error correction codes.

The barcode can comprise a target-binding region. The target-binding region can interact with a target in a sample. The target can be, or comprise, ribonucleic acids (RNAs), messenger RNAs (mRNAs), microRNAs, small interfering RNAs (siRNAs), RNA degradation products, RNAs each comprising a poly(A) tail, or any combination thereof. In some embodiments, the plurality of targets can include deoxyribonucleic acids (DNAs).

In some embodiments, a target-binding region can comprise an oligo(dT) sequence which can interact with poly(A) tails of mRNAs. One or more of the labels of the barcode (e.g., the universal label, the dimension label, the spatial label, the cell label, and the barcode sequences (e.g., molecular label)) can be separated by a spacer from another one or two of the remaining labels of the barcode. The spacer can be, for example, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20, or more nucleotides. In some embodiments, none of the labels of the barcode is separated by spacer.

Universal Labels

A barcode can comprise one or more universal labels. In some embodiments, the one or more universal labels can be the same for all barcodes in the set of barcodes attached to a given solid support. In some embodiments, the one or more universal labels can be the same for all barcodes attached to a plurality of beads. In some embodiments, a universal label can comprise a nucleic acid sequence that is capable of hybridizing to a sequencing primer. Sequencing primers can be used for sequencing barcodes comprising a universal label. Sequencing primers (e.g., universal sequencing primers) can comprise sequencing primers associated with high-throughput sequencing platforms. In some embodiments, a universal label can comprise a nucleic acid sequence that is capable of hybridizing to a PCR primer. In some embodiments, the universal label can comprise a nucleic acid sequence that is capable of hybridizing to a sequencing primer and a PCR primer. The nucleic acid sequence of the universal label that is capable of hybridizing to a sequencing or PCR primer can be referred to as a primer binding site. A universal label can comprise a sequence that can be used to initiate transcription of the barcode. A universal label can comprise a sequence that can be used for extension of the barcode or a region within the barcode. A universal label can be, or be about, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, or a number or a range between any two of these values, nucleotides in length. For example, a universal label can comprise at least about 10 nucleotides. A universal label can be at least, or be at most, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 100, 200, or 300 nucleotides in length. In some embodiments, a cleavable linker or modified nucleotide can be part of the universal label sequence to enable the barcode to be cleaved off from the support.

Dimension Labels

A barcode can comprise one or more dimension labels. In some embodiments, a dimension label can comprise a nucleic acid sequence that provides information about a dimension in which the labeling (e.g., stochastic labeling) occurred. For example, a dimension label can provide information about the time at which a target was barcoded. A dimension label can be associated with a time of barcoding (e.g., stochastic barcoding) in a sample. A dimension label can be activated at the time of labeling. Different dimension labels can be activated at different times. The dimension label provides information about the order in which targets, groups of targets, and/or samples were barcoded. For example, a population of cells can be barcoded at the G0 phase of the cell cycle. The cells can be pulsed again with barcodes (e.g., stochastic barcodes) at the G1 phase of the cell cycle. The cells can be pulsed again with barcodes at the S phase of the cell cycle, and so on. Barcodes at each pulse (e.g., each phase of the cell cycle), can comprise different dimension labels. In this way, the dimension label provides information about which targets were labelled at which phase of the cell cycle. Dimension labels can interrogate many different biological times. Exemplary biological times can include, but are not limited to, the cell cycle, transcription (e.g., transcription initiation), and transcript degradation. In another example, a sample (e.g., a cell, a population of cells) can be labeled before and/or after treatment with a drug and/or therapy. The changes in the number of copies of distinct targets can be indicative of the sample's response to the drug and/or therapy.

A dimension label can be activatable. An activatable dimension label can be activated at a specific time point. The activatable label can be, for example, constitutively activated (e.g., not turned off). The activatable dimension label can be, for example, reversibly activated (e.g., the activatable dimension label can be turned on and turned off). The dimension label can be, for example, reversibly activatable at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more times. The dimension label can be reversibly activatable, for example, at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more times. In some embodiments, the dimension label can be activated with fluorescence, light, a chemical event (e.g., cleavage, ligation of another molecule, addition of modifications (e.g., pegylated, sumoylated, acetylated, methylated, deacetylated, demethylated), a photochemical event (e.g., photocaging), and introduction of a non-natural nucleotide.

The dimension label can, in some embodiments, be identical for all barcodes (e.g., stochastic barcodes) attached to a given solid support (e.g., a bead), but different for different solid supports (e.g., beads). In some embodiments, at least 60%, 70%, 80%, 85%, 90%, 95%, 97%, 99% or 100%, of barcodes on the same solid support can comprise the same dimension label. In some embodiments, at least 60% of barcodes on the same solid support can comprise the same dimension label. In some embodiments, at least 95% of barcodes on the same solid support can comprise the same dimension label.

There can be as many as 10⁶ or more unique dimension label sequences represented in a plurality of solid supports (e.g., beads). A dimension label can be, or be about 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, or a number or a range between any two of these values, nucleotides in length. A dimension label can be at least, or be at most, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 100, 200, or 300, nucleotides in length. A dimension label can comprise between about 5 to about 200 nucleotides. A dimension label can comprise between about 10 to about 150 nucleotides. A dimension label can comprise between about 20 to about 125 nucleotides in length.

Spatial Labels

A barcode can comprise one or more spatial labels. In some embodiments, a spatial label can comprise a nucleic acid sequence that provides information about the spatial orientation of a target molecule which is associated with the barcode. A spatial label can be associated with a coordinate in a sample. The coordinate can be a fixed coordinate. For example, a coordinate can be fixed in reference to a substrate. A spatial label can be in reference to a two or three-dimensional grid. A coordinate can be fixed in reference to a landmark. The landmark can be identifiable in space. A landmark can be a structure which can be imaged. A landmark can be a biological structure, for example an anatomical landmark. A landmark can be a cellular landmark, for instance an organelle. A landmark can be a non-natural landmark such as a structure with an identifiable identifier such as a color code, bar code, magnetic property, fluorescents, radioactivity, or a unique size or shape. A spatial label can be associated with a physical partition (e.g., A well, a container, or a droplet). In some embodiments, multiple spatial labels are used together to encode one or more positions in space.

The spatial label can be identical for all barcodes attached to a given solid support (e.g., a bead), but different for different solid supports (e.g., beads). In some embodiments, the percentage of barcodes on the same solid support comprising the same spatial label can be, or be about, 60%, 70%, 80%, 85%, 90%, 95%, 97%, 99%, 100%, or a number or a range between any two of these values. In some embodiments, the percentage of barcodes on the same solid support comprising the same spatial label can be at least, or be at most, 60%, 70%, 80%, 85%, 90%, 95%, 97%, 99%, or 100%. In some embodiments, at least 60% of barcodes on the same solid support can comprise the same spatial label. In some embodiments, at least 95% of barcodes on the same solid support can comprise the same spatial label.

There can be as many as 10⁶ or more unique spatial label sequences represented in a plurality of solid supports (e.g., beads). A spatial label can be, or be about, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, or a number or a range between any two of these values, nucleotides in length. A spatial label can be at least or at most 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 100, 200, or 300 nucleotides in length. A spatial label can comprise between about 5 to about 200 nucleotides. A spatial label can comprise between about 10 to about 150 nucleotides. A spatial label can comprise between about 20 to about 125 nucleotides in length.

Cell Labels

A barcode (e.g., a stochastic barcode) can comprise one or more cell labels. In some embodiments, a cell label can comprise a nucleic acid sequence that provides information for determining which target nucleic acid originated from which cell. In some embodiments, the cell label is identical for all barcodes attached to a given solid support (e.g., a bead), but different for different solid supports (e.g., beads). In some embodiments, the percentage of barcodes on the same solid support comprising the same cell label can be, or be about 60%, 70%, 80%, 85%, 90%, 95%, 97%, 99%, 100%, or a number or a range between any two of these values. In some embodiments, the percentage of barcodes on the same solid support comprising the same cell label can be, or be about 60%, 70%, 80%, 85%, 90%, 95%, 97%, 99%, or 100%. For example, at least 60% of barcodes on the same solid support can comprise the same cell label. As another example, at least 95% of barcodes on the same solid support can comprise the same cell label.

There can be as many as 10⁶ or more unique cell label sequences represented in a plurality of solid supports (e.g., beads). A cell label can be, or be about, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, or a number or a range between any two of these values, nucleotides in length. A cell label can be at least, or be at most, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 100, 200, or 300 nucleotides in length. For example, a cell label can comprise between about 5 to about 200 nucleotides. As another example, a cell label can comprise between about 10 to about 150 nucleotides. As yet another example, a cell label can comprise between about 20 to about 125 nucleotides in length.

Barcode Sequences

A barcode can comprise one or more barcode sequences. In some embodiments, a barcode sequence can comprise a nucleic acid sequence that provides identifying information for the specific type of target nucleic acid species hybridized to the barcode. A barcode sequence can comprise a nucleic acid sequence that provides a counter (e.g., that provides a rough approximation) for the specific occurrence of the target nucleic acid species hybridized to the barcode (e.g., target-binding region).

In some embodiments, a diverse set of barcode sequences are attached to a given solid support (e.g., a bead). In some embodiments, there can be, or be about, 10², 10³, 10⁴, 10⁵, 10⁶, 10⁷, 10⁸, 10⁹, or a number or a range between any two of these values, unique molecular label sequences. For example, a plurality of barcodes can comprise about 6561 barcodes sequences with distinct sequences. As another example, a plurality of barcodes can comprise about 65536 barcode sequences with distinct sequences. In some embodiments, there can be at least, or be at most, 10², 10³, 10⁴, 10⁵, 10⁶, 10⁷, 10⁸, or 10⁹, unique barcode sequences. The unique molecular label sequences can be attached to a given solid support (e.g., a bead). In some embodiments, the unique molecular label sequence is partially or entirely encompassed by a particle (e.g., a hydrogel bead).

The length of a barcode can be different in different implementations. For example, a barcode can be, or be about, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, or a number or a range between any two of these values, nucleotides in length. As another example, a barcode can be at least, or be at most, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 100, 200, or 300 nucleotides in length.

Molecular Labels

A barcode (e.g., a stochastic barcode) can comprise one or more molecular labels. Molecular labels can include barcode sequences. In some embodiments, a molecular label can comprise a nucleic acid sequence that provides identifying information for the specific type of target nucleic acid species hybridized to the barcode. A molecular label can comprise a nucleic acid sequence that provides a counter for the specific occurrence of the target nucleic acid species hybridized to the barcode (e.g., target-binding region).

In some embodiments, a diverse set of molecular labels are attached to a given solid support (e.g., a bead). In some embodiments, there can be, or be about, 10², 10³, 10⁴, 10⁵, 10⁶, 10⁷, 10⁸, 10⁹, or a number or a range between any two of these values, of unique molecular label sequences. For example, a plurality of barcodes can comprise about 6561 molecular labels with distinct sequences. As another example, a plurality of barcodes can comprise about 65536 molecular labels with distinct sequences. In some embodiments, there can be at least, or be at most, 10², 10³, 10⁴, 10⁵, 10⁶, 10⁷, 10⁸, or 10⁹, unique molecular label sequences. Barcodes with unique molecular label sequences can be attached to a given solid support (e.g., a bead).

For barcoding (e.g., stochastic barcoding) using a plurality of stochastic barcodes, the ratio of the number of different molecular label sequences and the number of occurrence of any of the targets can be, or be about, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 30:1, 40:1, 50:1, 60:1, 70:1, 80:1, 90:1, 100:1, or a number or a range between any two of these values. A target can be an mRNA species comprising mRNA molecules with identical or nearly identical sequences. In some embodiments, the ratio of the number of different molecular label sequences and the number of occurrence of any of the targets is at least, or is at most, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 1:1, 16:1, 17:1, 18:1, 19:1, 20:1, 30:1, 40:1, 50:1, 60:1, 70:1, 80:1, 90:1, or 100:1.

A molecular label can be, or be about, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, or a number or a range between any two of these values, nucleotides in length. A molecular label can be at least, or be at most, 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 100, 200, or 300 nucleotides in length.

Target-Binding Region

A barcode can comprise one or more target binding regions, such as capture probes. In some embodiments, a target-binding region can hybridize with a target of interest. In some embodiments, the target binding regions can comprise a nucleic acid sequence that hybridizes specifically to a target (e.g., target nucleic acid, target molecule, e.g., a cellular nucleic acid to be analyzed), for example to a specific gene sequence. In some embodiments, a target binding region can comprise a nucleic acid sequence that can attach (e.g., hybridize) to a specific location of a specific target nucleic acid. In some embodiments, the target binding region can comprise a nucleic acid sequence that is capable of specific hybridization to a restriction enzyme site overhang (e.g., an EcoRI sticky-end overhang). The barcode can then ligate to any nucleic acid molecule comprising a sequence complementary to the restriction site overhang.

In some embodiments, a target binding region can comprise a non-specific target nucleic acid sequence. A non-specific target nucleic acid sequence can refer to a sequence that can bind to multiple target nucleic acids, independent of the specific sequence of the target nucleic acid. For example, target binding region can comprise a random multimer sequence, a poly(dA) sequence, a poly(dT) sequence, a poly(dG) sequence, a poly(dC) sequence, or a combination thereof. For example, the target binding region can be an oligo(dT) sequence that hybridizes to the poly(A) tail on mRNA molecules. A random multimer sequence can be, for example, a random dimer, trimer, quatramer, pentamer, hexamer, septamer, octamer, nonamer, decamer, or higher multimer sequence of any length. In some embodiments, the target binding region is the same for all barcodes attached to a given bead. In some embodiments, the target binding regions for the plurality of barcodes attached to a given bead can comprise two or more different target binding sequences. A target binding region can be, or be about, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, or a number or a range between any two of these values, nucleotides in length. A target binding region can be at most about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. For example, an mRNA molecule can be reverse transcribed using a reverse transcriptase, such as Moloney murine leukemia virus (MMLV) reverse transcriptase, to generate a cDNA molecule with a poly(dC) tail. A barcode can include a target binding region with a poly(dG) tail. Upon base pairing between the poly(dG) tail of the barcode and the poly(dC) tail of the cDNA molecule, the reverse transcriptase switches template strands, from cellular RNA molecule to the barcode, and continues replication to the 5′ end of the barcode. By doing so, the resulting cDNA molecule contains the sequence of the barcode (such as the molecular label) on the 3′ end of the cDNA molecule.

In some embodiments, a target-binding region can comprise an oligo(dT) which can hybridize with mRNAs comprising polyadenylated ends. A target-binding region can be gene-specific. For example, a target-binding region can be configured to hybridize to a specific region of a target. A target-binding region can be, or be about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 27, 28, 29, 30, or a number or a range between any two of these values, nucleotides in length. A target-binding region can be at least, or be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 27, 28, 29, or 30, nucleotides in length. A target-binding region can be about 5-30 nucleotides in length. When a barcode comprises a gene-specific target-binding region, the barcode can be referred to herein as a gene-specific barcode.

Orientation Property

A stochastic barcode (e.g., a stochastic barcode) can comprise one or more orientation properties which can be used to orient (e.g., align) the barcodes. A barcode can comprise a moiety for isoelectric focusing. Different barcodes can comprise different isoelectric focusing points. When these barcodes are introduced to a sample, the sample can undergo isoelectric focusing in order to orient the barcodes into a known way. In this way, the orientation property can be used to develop a known map of barcodes in a sample. Exemplary orientation properties can include, electrophoretic mobility (e.g., based on size of the barcode), isoelectric point, spin, conductivity, and/or self-assembly. For example, barcodes with an orientation property of self-assembly, can self-assemble into a specific orientation (e.g., nucleic acid nanostructure) upon activation.

Affinity Property

A barcode (e.g., a stochastic barcode) can comprise one or more affinity properties. For example, a spatial label can comprise an affinity property. An affinity property can include a chemical and/or biological moiety that can facilitate binding of the barcode to another entity (e.g., cell receptor). For example, an affinity property can comprise an antibody, for example, an antibody specific for a specific moiety (e.g., receptor) on a sample. In some embodiments, the antibody can guide the barcode to a specific cell type or molecule. Targets at and/or near the specific cell type or molecule can be labeled (e.g., stochastically labeled). The affinity property can, in some embodiments, provide spatial information in addition to the nucleotide sequence of the spatial label because the antibody can guide the barcode to a specific location. The antibody can be a therapeutic antibody, for example a monoclonal antibody or a polyclonal antibody. The antibody can be humanized or chimeric. The antibody can be a naked antibody or a fusion antibody.

The antibody can be a full-length (i.e., naturally occurring or formed by normal immunoglobulin gene fragment recombinatorial processes) immunoglobulin molecule (e.g., an IgG antibody) or an immunologically active (i.e., specifically binding) portion of an immunoglobulin molecule, like an antibody fragment.

The antibody fragment can be, for example, a portion of an antibody such as F(ab′)2, Fab′, Fab, Fv, sFv and the like. In some embodiments, the antibody fragment can bind with the same antigen that is recognized by the full-length antibody. The antibody fragment can include isolated fragments consisting of the variable regions of antibodies, such as the “Fv” fragments consisting of the variable regions of the heavy and light chains and recombinant single chain polypeptide molecules in which light and heavy variable regions are connected by a peptide linker (“scFv proteins”). Exemplary antibodies can include, but are not limited to, antibodies for cancer cells, antibodies for viruses, antibodies that bind to cell surface receptors (CD8, CD34, CD45), and therapeutic antibodies.

Universal Adaptor Primer

A barcode can comprise one or more universal adaptor primers. For example, a gene-specific barcode, such as a gene-specific stochastic barcode, can comprise a universal adaptor primer. A universal adaptor primer can refer to a nucleotide sequence that is universal across all barcodes. A universal adaptor primer can be used for building gene-specific barcodes. A universal adaptor primer can be, or be about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 27, 28, 29, 30, or a number or a range between any two of these nucleotides in length. A universal adaptor primer can be at least, or be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 27, 28, 29, or 30 nucleotides in length. A universal adaptor primer can be from 5-30 nucleotides in length.

Linker

When a barcode comprises more than one of a type of label (e.g., more than one cell label or more than one barcode sequence, such as one molecular label), the labels may be interspersed with a linker label sequence. A linker label sequence can be at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. A linker label sequence can be at most about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. In some instances, a linker label sequence is 12 nucleotides in length. A linker label sequence can be used to facilitate the synthesis of the barcode. The linker label can comprise an error-correcting (e.g., Hamming) code.

Solid Supports

Barcodes, such as stochastic barcodes, disclosed herein can, in some embodiments, be associated with a solid support. The solid support can be, for example, a synthetic particle. In some embodiments, some or all of the barcode sequences, such as molecular labels for stochastic barcodes (e.g., the first barcode sequences) of a plurality of barcodes (e.g., the first plurality of barcodes) on a solid support differ by at least one nucleotide. The cell labels of the barcodes on the same solid support can be the same. The cell labels of the barcodes on different solid supports can differ by at least one nucleotide. For example, first cell labels of a first plurality of barcodes on a first solid support can have the same sequence, and second cell labels of a second plurality of barcodes on a second solid support can have the same sequence. The first cell labels of the first plurality of barcodes on the first solid support and the second cell labels of the second plurality of barcodes on the second solid support can differ by at least one nucleotide. A cell label can be, for example, about 5-20 nucleotides long. A barcode sequence can be, for example, about 5-20 nucleotides long. The synthetic particle can be, for example, a bead.

The bead can be, for example, a silica gel bead, a controlled pore glass bead, a magnetic bead, a Dynabead, a Sephadex/Sepharose bead, a cellulose bead, a polystyrene bead, or any combination thereof. The bead can comprise a material such as polydimethylsiloxane (PDMS), polystyrene, glass, polypropylene, agarose, gelatin, hydrogel, paramagnetic, ceramic, plastic, glass, methylstyrene, acrylic polymer, titanium, latex, Sepharose, cellulose, nylon, silicone, or any combination thereof.

In some embodiments, the bead can be a polymeric bead, for example a deformable bead or a gel bead, functionalized with barcodes or stochastic barcodes (such as gel beads from 10X Genomics (San Francisco, Calif.). In some implementation, a gel bead can comprise a polymer based gels. Gel beads can be generated, for example, by encapsulating one or more polymeric precursors into droplets. Upon exposure of the polymeric precursors to an accelerator (e.g., tetramethylethylenediamine (TEMED)), a gel bead may be generated.

In some embodiments, the particle can be disruptable (e.g., dissolvable, degradable). For example, the polymeric bead can dissolve, melt, or degrade, for example, under a desired condition. The desired condition can include an environmental condition. The desired condition may result in the polymeric bead dissolving, melting, or degrading in a controlled manner. A gel bead may dissolve, melt, or degrade due to a chemical stimulus, a physical stimulus, a biological stimulus, a thermal stimulus, a magnetic stimulus, an electric stimulus, a light stimulus, or any combination thereof.

Analytes and/or reagents, such as oligonucleotide barcodes, for example, may be coupled/immobilized to the interior surface of a gel bead (e.g., the interior accessible via diffusion of an oligonucleotide barcode and/or materials used to generate an oligonucleotide barcode) and/or the outer surface of a gel bead or any other microcapsule described herein. Coupling/immobilization may be via any form of chemical bonding (e.g., covalent bond, ionic bond) or physical phenomena (e.g., Van der Waals forces, dipole-dipole interactions, etc.). In some embodiments, coupling/immobilization of a reagent to a gel bead or any other microcapsule described herein may be reversible, such as, for example, via a labile moiety (e.g., via a chemical cross-linker, including chemical cross-linkers described herein). Upon application of a stimulus, the labile moiety may be cleaved and the immobilized reagent set free. In some embodiments, the labile moiety is a disulfide bond. For example, in the case where an oligonucleotide barcode is immobilized to a gel bead via a disulfide bond, exposure of the disulfide bond to a reducing agent can cleave the disulfide bond and free the oligonucleotide barcode from the bead. The labile moiety may be included as part of a gel bead or microcapsule, as part of a chemical linker that links a reagent or analyte to a gel bead or microcapsule, and/or as part of a reagent or analyte. In some embodiments, at least one barcode of the plurality of barcodes can be immobilized on the particle, partially immobilized on the particle, enclosed in the particle, partially enclosed in the particle, or any combination thereof.

In some embodiments, a gel bead can comprise a wide range of different polymers including but not limited to: polymers, heat sensitive polymers, photosensitive polymers, magnetic polymers, pH sensitive polymers, salt-sensitive polymers, chemically sensitive polymers, polyelectrolytes, polysaccharides, peptides, proteins, and/or plastics. Polymers may include but are not limited to materials such as poly(N-isopropylacrylamide) (PNIPAAm), poly(styrene sulfonate) (PSS), poly(allyl amine) (PAAm), poly(acrylic acid) (PAA), poly(ethylene imine) (PEI), poly(diallyldimethyl-ammonium chloride) (PDADMAC), poly(pyrolle) (PPy), poly(vinylpyrrolidone) (PVPON), poly(vinyl pyridine) (PVP), poly(methacrylic acid) (PMAA), poly(methyl methacrylate) (PMMA), polystyrene (PS), poly(tetrahydrofuran) (PTHF), poly(phthaladehyde) (PTHF), poly(hexyl viologen) (PHV), poly(L-lysine) (PLL), poly(L-arginine) (PARG), poly(lactic-co-glycolic acid) (PLGA).

Numerous chemical stimuli can be used to trigger the disruption, dissolution, or degradation of the beads. Examples of these chemical changes may include, but are not limited to pH-mediated changes to the bead wall, disintegration of the bead wall via chemical cleavage of crosslink bonds, triggered depolymerization of the bead wall, and bead wall switching reactions. Bulk changes may also be used to trigger disruption of the beads.

Bulk or physical changes to the microcapsule through various stimuli also offer many advantages in designing capsules to release reagents. Bulk or physical changes occur on a macroscopic scale, in which bead rupture is the result of mechano-physical forces induced by a stimulus. These processes may include, but are not limited to pressure induced rupture, bead wall melting, or changes in the porosity of the bead wall.

Biological stimuli may also be used to trigger disruption, dissolution, or degradation of beads. Generally, biological triggers resemble chemical triggers, but many examples use biomolecules, or molecules commonly found in living systems such as enzymes, peptides, saccharides, fatty acids, nucleic acids and the like. For example, beads may comprise polymers with peptide cross-links that are sensitive to cleavage by specific proteases. More specifically, one example may comprise a microcapsule comprising GFLGK peptide cross links. Upon addition of a biological trigger such as the protease Cathepsin B, the peptide cross links of the shell well are cleaved and the contents of the beads are released. In other cases, the proteases may be heat-activated. In another example, beads comprise a shell wall comprising cellulose. Addition of the hydrolytic enzyme chitosan serves as biologic trigger for cleavage of cellulosic bonds, depolymerization of the shell wall, and release of its inner contents.

The beads may also be induced to release their contents upon the application of a thermal stimulus. A change in temperature can cause a variety changes to the beads. A change in heat may cause melting of a bead such that the bead wall disintegrates. In other cases, the heat may increase the internal pressure of the inner components of the bead such that the bead ruptures or explodes. In still other cases, the heat may transform the bead into a shrunken dehydrated state. The heat may also act upon heat-sensitive polymers within the wall of a bead to cause disruption of the bead.

Inclusion of magnetic nanoparticles to the bead wall of microcapsules may allow triggered rupture of the beads as well as guide the beads in an array. A device of this disclosure may comprise magnetic beads for either purpose. In one example, incorporation of Fe₃O₄ nanoparticles into polyelectrolyte containing beads triggers rupture in the presence of an oscillating magnetic field stimulus.

A bead may also be disrupted, dissolved, or degraded as the result of electrical stimulation. Similar to magnetic particles described in the previous section, electrically sensitive beads can allow for both triggered rupture of the beads as well as other functions such as alignment in an electric field, electrical conductivity or redox reactions. In one example, beads containing electrically sensitive material are aligned in an electric field such that release of inner reagents can be controlled. In other examples, electrical fields may induce redox reactions within the bead wall itself that may increase porosity.

A light stimulus may also be used to disrupt the beads. Numerous light triggers are possible and may include systems that use various molecules such as nanoparticles and chromophores capable of absorbing photons of specific ranges of wavelengths. For example, metal oxide coatings can be used as capsule triggers. UV irradiation of polyelectrolyte capsules coated with SiO₂ may result in disintegration of the bead wall. In yet another example, photo switchable materials such as azobenzene groups may be incorporated in the bead wall. Upon the application of UV or visible light, chemicals such as these undergo a reversible cis-to-trans isomerization upon absorption of photons. In this aspect, incorporation of photon switches result in a bead wall that may disintegrate or become more porous upon the application of a light trigger.

For example, in a non-limiting example of barcoding (e.g., stochastic barcoding) illustrated in FIG. 2, after introducing cells such as single cells onto a plurality of microwells of a microwell array at block 208, beads can be introduced onto the plurality of microwells of the microwell array at block 212. Each microwell can comprise one bead. The beads can comprise a plurality of barcodes. A barcode can comprise a 5′ amine region attached to a bead. The barcode can comprise a universal label, a barcode sequence (e.g., a molecular label), a target-binding region, or any combination thereof.

The barcodes disclosed herein can be associated with (e.g., attached to) a solid support (e.g., a bead). The barcodes associated with a solid support can each comprise a barcode sequence selected from a group comprising at least 100 or 1000 barcode sequences with unique sequences. In some embodiments, different barcodes associated with a solid support can comprise barcode with different sequences. In some embodiments, a percentage of barcodes associated with a solid support comprises the same cell label. For example, the percentage can be, or be about 60%, 70%, 80%, 85%, 90%, 95%, 97%, 99%, 100%, or a number or a range between any two of these values. As another example, the percentage can be at least, or be at most 60%, 70%, 80%, 85%, 90%, 95%, 97%, 99%, or 100%. In some embodiments, barcodes associated with a solid support can have the same cell label. The barcodes associated with different solid supports can have different cell labels selected from a group comprising at least 100 or 1000 cell labels with unique sequences.

The barcodes disclosed herein can be associated to (e.g., attached to) a solid support (e.g., a bead). In some embodiments, barcoding the plurality of targets in the sample can be performed with a solid support including a plurality of synthetic particles associated with the plurality of barcodes. In some embodiments, the solid support can include a plurality of synthetic particles associated with the plurality of barcodes. The spatial labels of the plurality of barcodes on different solid supports can differ by at least one nucleotide. The solid support can, for example, include the plurality of barcodes in two dimensions or three dimensions. The synthetic particles can be beads. The beads can be silica gel beads, controlled pore glass beads, magnetic beads, Dynabeads, Sephadex/Sepharose beads, cellulose beads, polystyrene beads, or any combination thereof. The solid support can include a polymer, a matrix, a hydrogel, a needle array device, an antibody, or any combination thereof. In some embodiments, the solid supports can be free floating. In some embodiments, the solid supports can be embedded in a semi-solid or solid array. The barcodes may not be associated with solid supports. The barcodes can be individual nucleotides. The barcodes can be associated with a substrate.

As used herein, the terms “tethered,” “attached,” and “immobilized,” are used interchangeably, and can refer to covalent or non-covalent means for attaching barcodes to a solid support. Any of a variety of different solid supports can be used as solid supports for attaching pre-synthesized barcodes or for in situ solid-phase synthesis of barcode.

In some embodiments, the solid support is a bead. The bead can comprise one or more types of solid, porous, or hollow sphere, ball, bearing, cylinder, or other similar configuration which a nucleic acid can be immobilized (e.g., covalently or non-covalently). The bead can be, for example, composed of plastic, ceramic, metal, polymeric material, or any combination thereof. A bead can be, or comprise, a discrete particle that is spherical (e.g., microspheres) or have a non-spherical or irregular shape, such as cubic, cuboid, pyramidal, cylindrical, conical, oblong, or disc-shaped, and the like. In some embodiments, a bead can be non-spherical in shape.

Beads can comprise a variety of materials including, but not limited to, paramagnetic materials (e.g., magnesium, molybdenum, lithium, and tantalum), superparamagnetic materials (e.g., ferrite (Fe₃O₄; magnetite) nanoparticles), ferromagnetic materials (e.g., iron, nickel, cobalt, some alloys thereof, and some rare earth metal compounds), ceramic, plastic, glass, polystyrene, silica, methylstyrene, acrylic polymers, titanium, latex, Sepharose, agarose, hydrogel, polymer, cellulose, nylon, or any combination thereof.

In some embodiments, the bead (e.g., the bead to which the labels are attached) is a hydrogel bead. In some embodiments, the bead comprises hydrogel.

Some embodiments disclosed herein include one or more particles (for example, beads). Each of the particles can comprise a plurality of oligonucleotides (e.g., barcodes). Each of the plurality of oligonucleotides can comprise a barcode sequence (e.g., a molecular label sequence), a cell label, and a target-binding region (e.g., an oligo(dT) sequence, a gene-specific sequence, a random multimer, or a combination thereof). The cell label sequence of each of the plurality of oligonucleotides can be the same. The cell label sequences of oligonucleotides on different particles can be different such that the oligonucleotides on different particles can be identified. The number of different cell label sequences can be different in different implementations. In some embodiments, the number of cell label sequences can be, or be about 10, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000, 20000, 30000, 40000, 50000, 60000, 70000, 80000, 90000, 100000, 10⁶, 10⁷, 10⁸, 10⁹, a number or a range between any two of these values, or more. In some embodiments, the number of cell label sequences can be at least, or be at most 10, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000, 20000, 30000, 40000, 50000, 60000, 70000, 80000, 90000, 100000, 10⁶, 10⁷, 10⁸, or 10⁹. In some embodiments, no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, or more of the plurality of the particles include oligonucleotides with the same cell sequence. In some embodiment, the plurality of particles that include oligonucleotides with the same cell sequence can be at most 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, or more. In some embodiments, none of the plurality of the particles has the same cell label sequence.

The plurality of oligonucleotides on each particle can comprise different barcode sequences (e.g., molecular labels). In some embodiments, the number of barcode sequences can be, or be about 10, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000, 20000, 30000, 40000, 50000, 60000, 70000, 80000, 90000, 100000, 10⁶, 10⁷, 10⁸, 10⁹, or a number or a range between any two of these values. In some embodiments, the number of barcode sequences can be at least, or be at most 10, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000, 20000, 30000, 40000, 50000, 60000, 70000, 80000, 90000, 100000, 10⁶, 10⁷, 10⁸, or 10⁹. For example, at least 100 of the plurality of oligonucleotides comprise different barcode sequences. As another example, in a single particle, at least 100, 500, 1000, 5000, 10000, 15000, 20000, 50000, a number or a range between any two of these values, or more of the plurality of oligonucleotides comprise different barcode sequences. Some embodiments provide a plurality of the particles comprising barcodes. In some embodiments, the ratio of an occurrence (or a copy or a number) of a target to be labeled and the different barcode sequences can be at least 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:30, 1:40, 1:50, 1:60, 1:70, 1:80, 1:90, or more. In some embodiments, each of the plurality of oligonucleotides further comprises a sample label, a universal label, or both. The particle can be, for example, a nanoparticle or microparticle.

The size of the beads can vary. For example, the diameter of the bead can range from 0.1 micrometer to 50 micrometer. In some embodiments, the diameter of the bead can be, or be about, 0.1, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50 micrometer, or a number or a range between any two of these values.

The diameter of the bead can be related to the diameter of the wells of the substrate. In some embodiments, the diameter of the bead can be, or be about, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, or a number or a range between any two of these values, longer or shorter than the diameter of the well. The diameter of the beads can be related to the diameter of a cell (e.g., a single cell entrapped by a well of the substrate). In some embodiments, the diameter of the bead can be at least, or be at most, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100% longer or shorter than the diameter of the well. The diameter of the beads can be related to the diameter of a cell (e.g., a single cell entrapped by a well of the substrate). In some embodiments, the diameter of the bead can be, or be about, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 150%, 200%, 250%, 300%, or a number or a range between any two of these values, longer or shorter than the diameter of the cell. In some embodiments, the diameter of the beads can be at least, or be at most, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 150%, 200%, 250%, or 300% longer or shorter than the diameter of the cell.

A bead can be attached to and/or embedded in a substrate. A bead can be attached to and/or embedded in a gel, hydrogel, polymer and/or matrix. The spatial position of a bead within a substrate (e.g., gel, matrix, scaffold, or polymer) can be identified using the spatial label present on the barcode on the bead which can serve as a location address.

Examples of beads can include, but are not limited to, streptavidin beads, agarose beads, magnetic beads, Dynabeads®, MACS® microbeads, antibody conjugated beads (e.g., anti-immunoglobulin microbeads), protein A conjugated beads, protein G conjugated beads, protein A/G conjugated beads, protein L conjugated beads, oligo(dT) conjugated beads, silica beads, silica-like beads, anti-biotin microbeads, anti-fluorochrome microbeads, and BcMag™ Carboxyl-Terminated Magnetic Beads.

A bead can be associated with (e.g., impregnated with) quantum dots or fluorescent dyes to make it fluorescent in one fluorescence optical channel or multiple optical channels. A bead can be associated with iron oxide or chromium oxide to make it paramagnetic or ferromagnetic. Beads can be identifiable. For example, a bead can be imaged using a camera. A bead can have a detectable code associated with the bead. For example, a bead can comprise a barcode. A bead can change size, for example, due to swelling in an organic or inorganic solution. A bead can be hydrophobic. A bead can be hydrophilic. A bead can be biocompatible.

A solid support (e.g., a bead) can be visualized. The solid support can comprise a visualizing tag (e.g., fluorescent dye). A solid support (e.g., a bead) can be etched with an identifier (e.g., a number). The identifier can be visualized through imaging the beads.

A solid support can comprise an insoluble, semi-soluble, or insoluble material. A solid support can be referred to as “functionalized” when it includes a linker, a scaffold, a building block, or other reactive moiety attached thereto, whereas a solid support may be “nonfunctionalized” when it lack such a reactive moiety attached thereto. The solid support can be employed free in solution, such as in a microtiter well format; in a flow-through format, such as in a column; or in a dipstick.

The solid support can comprise a membrane, paper, plastic, coated surface, flat surface, glass, slide, chip, or any combination thereof. A solid support can take the form of resins, gels, microspheres, or other geometric configurations. A solid support can comprise silica chips, microparticles, nanoparticles, plates, arrays, capillaries, flat supports such as glass fiber filters, glass surfaces, metal surfaces (steel, gold silver, aluminum, silicon and copper), glass supports, plastic supports, silicon supports, chips, filters, membranes, microwell plates, slides, plastic materials including multiwell plates or membranes (e.g., formed of polyethylene, polypropylene, polyamide, polyvinylidenedifluoride), and/or wafers, combs, pins or needles (e.g., arrays of pins suitable for combinatorial synthesis or analysis) or beads in an array of pits or nanoliter wells of flat surfaces such as wafers (e.g., silicon wafers), wafers with pits with or without filter bottoms.

The solid support can comprise a polymer matrix (e.g., gel, hydrogel). The polymer matrix may be able to permeate intracellular space (e.g., around organelles). The polymer matrix may able to be pumped throughout the circulatory system.

Substrates and Microwell Array

As used herein, a substrate can refer to a type of solid support. A substrate can refer to a solid support that can comprise barcodes or stochastic barcodes of the disclosure. A substrate can, for example, comprise a plurality of microwells. For example, a substrate can be a well array comprising two or more microwells. In some embodiments, a microwell can comprise a small reaction chamber of defined volume. In some embodiments, a microwell can entrap one or more cells. In some embodiments, a microwell can entrap only one cell. In some embodiments, a microwell can entrap one or more solid supports. In some embodiments, a microwell can entrap only one solid support. In some embodiments, a microwell entraps a single cell and a single solid support (e.g., a bead). A microwell can comprise barcode reagents of the disclosure.

Methods of Barcoding

The disclosure provides for methods for estimating the number of distinct targets at distinct locations in a physical sample (e.g., tissue, organ, tumor, cell). The methods can comprise placing barcodes (e.g., stochastic barcodes) in close proximity with the sample, lysing the sample, associating distinct targets with the barcodes, amplifying the targets and/or digitally counting the targets. The method can further comprise analyzing and/or visualizing the information obtained from the spatial labels on the barcodes. In some embodiments, a method comprises visualizing the plurality of targets in the sample. Mapping the plurality of targets onto the map of the sample can include generating a two dimensional map or a three dimensional map of the sample. The two dimensional map and the three dimensional map can be generated prior to or after barcoding (e.g., stochastically barcoding) the plurality of targets in the sample. Visualizing the plurality of targets in the sample can include mapping the plurality of targets onto a map of the sample. Mapping the plurality of targets onto the map of the sample can include generating a two dimensional map or a three dimensional map of the sample. The two dimensional map and the three dimensional map can be generated prior to or after barcoding the plurality of targets in the sample. in some embodiments, the two dimensional map and the three dimensional map can be generated before or after lysing the sample. Lysing the sample before or after generating the two dimensional map or the three dimensional map can include heating the sample, contacting the sample with a detergent, changing the pH of the sample, or any combination thereof.

In some embodiments, barcoding the plurality of targets comprises hybridizing a plurality of barcodes with a plurality of targets to create barcoded targets (e.g., stochastically barcoded targets). Barcoding the plurality of targets can comprise generating an indexed library of the barcoded targets. Generating an indexed library of the barcoded targets can be performed with a solid support comprising the plurality of barcodes (e.g., stochastic barcodes).

Contacting a Sample and a Barcode

The disclosure provides for methods for contacting a sample (e.g., cells) to a substrate of the disclosure. A sample comprising, for example, a cell, organ, or tissue thin section, can be contacted to barcodes (e.g., stochastic barcodes). The cells can be contacted, for example, by gravity flow wherein the cells can settle and create a monolayer. The sample can be a tissue thin section. The thin section can be placed on the substrate. The sample can be one-dimensional (e.g., forms a planar surface). The sample (e.g., cells) can be spread across the substrate, for example, by growing/culturing the cells on the substrate.

When barcodes are in close proximity to targets, the targets can hybridize to the barcode. The barcodes can be contacted at a non-depletable ratio such that each distinct target can associate with a distinct barcode of the disclosure. To ensure efficient association between the target and the barcode, the targets can be cross-linked to barcode.

Cell Lysis

Following the distribution of cells and barcodes, the cells can be lysed to liberate the target molecules. Cell lysis can be accomplished by any of a variety of means, for example, by chemical or biochemical means, by osmotic shock, or by means of thermal lysis, mechanical lysis, or optical lysis. Cells can be lysed by addition of a cell lysis buffer comprising a detergent (e.g., SDS, Li dodecyl sulfate, Triton X-100, Tween-20, or NP-40), an organic solvent (e.g., methanol or acetone), or digestive enzymes (e.g., proteinase K, pepsin, or trypsin), or any combination thereof. To increase the association of a target and a barcode, the rate of the diffusion of the target molecules can be altered by for example, reducing the temperature and/or increasing the viscosity of the lysate.

In some embodiments, the sample can be lysed using a filter paper. The filter paper can be soaked with a lysis buffer on top of the filter paper. The filter paper can be applied to the sample with pressure which can facilitate lysis of the sample and hybridization of the targets of the sample to the substrate.

In some embodiments, lysis can be performed by mechanical lysis, heat lysis, optical lysis, and/or chemical lysis. Chemical lysis can include the use of digestive enzymes such as proteinase K, pepsin, and trypsin. Lysis can be performed by the addition of a lysis buffer to the substrate. A lysis buffer can comprise Tris HCl. A lysis buffer can comprise at least about 0.01, 0.05, 0.1, 0.5, or 1 M or more Tris HCl. A lysis buffer can comprise at most about 0.01, 0.05, 0.1, 0.5, or 1 M or more Tris HCL. A lysis buffer can comprise about 0.1 M Tris HCl. The pH of the lysis buffer can be at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more. The pH of the lysis buffer can be at most about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more. In some embodiments, the pH of the lysis buffer is about 7.5. The lysis buffer can comprise a salt (e.g., LiCl). The concentration of salt in the lysis buffer can be at least about 0.1, 0.5, or 1 M or more. The concentration of salt in the lysis buffer can be at most about 0.1, 0.5, or 1 M or more. In some embodiments, the concentration of salt in the lysis buffer is about 0.5M. The lysis buffer can comprise a detergent (e.g., SDS, Li dodecyl sulfate, triton X, tween, NP-40). The concentration of the detergent in the lysis buffer can be at least about 0.0001%, 0.0005%, 0.001%, 0.005%, 0.01%, 0.05%, 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, or 7%, or more. The concentration of the detergent in the lysis buffer can be at most about 0.0001%, 0.0005%, 0.001%, 0.005%, 0.01%, 0.05%, 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, or 7%, or more. In some embodiments, the concentration of the detergent in the lysis buffer is about 1% Li dodecyl sulfate. The time used in the method for lysis can be dependent on the amount of detergent used. In some embodiments, the more detergent used, the less time needed for lysis. The lysis buffer can comprise a chelating agent (e.g., EDTA, EGTA). The concentration of a chelating agent in the lysis buffer can be at least about 1, 5, 10, 15, 20, 25, or 30 mM or more. The concentration of a chelating agent in the lysis buffer can be at most about 1, 5, 10, 15, 20, 25, or 30 mM or more. In some embodiments, the concentration of chelating agent in the lysis buffer is about 10 mM. The lysis buffer can comprise a reducing reagent (e.g., beta-mercaptoethanol, DTT). The concentration of the reducing reagent in the lysis buffer can be at least about 1, 5, 10, 15, or 20 mM or more. The concentration of the reducing reagent in the lysis buffer can be at most about 1, 5, 10, 15, or 20 mM or more. In some embodiments, the concentration of reducing reagent in the lysis buffer is about 5 mM. In some embodiments, a lysis buffer can comprise about 0.1M TrisHCl, about pH 7.5, about 0.5M LiCl, about 1% lithium dodecyl sulfate, about 10 mM EDTA, and about 5 mM DTT.

Lysis can be performed at a temperature of about 4, 10, 15, 20, 25, or 30° C. Lysis can be performed for about 1, 5, 10, 15, or 20 or more minutes. A lysed cell can comprise at least about 100000, 200000, 300000, 400000, 500000, 600000, or 700000 or more target nucleic acid molecules. A lysed cell can comprise at most about 100000, 200000, 300000, 400000, 500000, 600000, or 700000 or more target nucleic acid molecules.

Attachment of Barcodes to Target Nucleic Acid Molecules

Following lysis of the cells and release of nucleic acid molecules therefrom, the nucleic acid molecules can randomly associate with the barcodes of the co-localized solid support. Association can comprise hybridization of a barcode's target recognition region to a complementary portion of the target nucleic acid molecule (e.g., oligo(dT) of the barcode can interact with a poly(A) tail of a target). The assay conditions used for hybridization (e.g., buffer pH, ionic strength, temperature, etc.) can be chosen to promote formation of specific, stable hybrids. In some embodiments, the nucleic acid molecules released from the lysed cells can associate with the plurality of probes on the substrate (e.g., hybridize with the probes on the substrate). When the probes comprise oligo(dT), mRNA molecules can hybridize to the probes and be reverse transcribed. The oligo(dT) portion of the oligonucleotide can act as a primer for first strand synthesis of the cDNA molecule. For example, in a non-limiting example of barcoding illustrated in FIG. 2, at block 216, mRNA molecules can hybridize to barcodes on beads. For example, single-stranded nucleotide fragments can hybridize to the target-binding regions of barcodes.

Attachment can further comprise ligation of a barcode's target recognition region and a portion of the target nucleic acid molecule. For example, the target binding region can comprise a nucleic acid sequence that can be capable of specific hybridization to a restriction site overhang (e.g., an EcoRI sticky-end overhang). The assay procedure can further comprise treating the target nucleic acids with a restriction enzyme (e.g., EcoRI) to create a restriction site overhang. The barcode can then be ligated to any nucleic acid molecule comprising a sequence complementary to the restriction site overhang. A ligase (e.g., T4 DNA ligase) can be used to join the two fragments.

For example, in a non-limiting example of barcoding illustrated in FIG. 2, at block 220, the labeled targets from a plurality of cells (or a plurality of samples) (e.g., target-barcode molecules) can be subsequently pooled, for example, into a tube. The labeled targets can be pooled by, for example, retrieving the barcodes and/or the beads to which the target-barcode molecules are attached.

The retrieval of solid support-based collections of attached target-barcode molecules can be implemented by use of magnetic beads and an externally-applied magnetic field. Once the target-barcode molecules have been pooled, all further processing can proceed in a single reaction vessel. Further processing can include, for example, reverse transcription reactions, amplification reactions, cleavage reactions, dissociation reactions, and/or nucleic acid extension reactions. Further processing reactions can be performed within the microwells, that is, without first pooling the labeled target nucleic acid molecules from a plurality of cells.

Reverse Transcription or Nucleic Acid Extension

The disclosure provides for a method to create a target-barcode conjugate using reverse transcription (e.g., at block 224 of FIG. 2) or nucleic acid extension. The target-barcode conjugate can comprise the barcode and a complementary sequence of all or a portion of the target nucleic acid (i.e., a barcoded cDNA molecule, such as a stochastically barcoded cDNA molecule). Reverse transcription of the associated RNA molecule can occur by the addition of a reverse transcription primer along with the reverse transcriptase. The reverse transcription primer can be an oligo(dT) primer, a random hexanucleotide primer, or a target-specific oligonucleotide primer. Oligo(dT) primers can be, or can be about, 12-18 nucleotides in length and bind to the endogenous poly(A) tail at the 3′ end of mammalian mRNA. Random hexanucleotide primers can bind to mRNA at a variety of complementary sites. Target-specific oligonucleotide primers typically selectively prime the mRNA of interest.

In some embodiments, reverse transcription of an mRNA molecule to a labeled-RNA molecule can occur by the addition of a reverse transcription primer. In some embodiments, the reverse transcription primer is an oligo(dT) primer, random hexanucleotide primer, or a target-specific oligonucleotide primer. Generally, oligo(dT) primers are 12-18 nucleotides in length and bind to the endogenous poly(A) tail at the 3′ end of mammalian mRNA. Random hexanucleotide primers can bind to mRNA at a variety of complementary sites. Target-specific oligonucleotide primers typically selectively prime the mRNA of interest.

In some embodiments, a target is a cDNA molecule. For example, an mRNA molecule can be reverse transcribed using a reverse transcriptase, such as Moloney murine leukemia virus (MMLV) reverse transcriptase, to generate a cDNA molecule with a poly(dC) tail. A barcode can include a target binding region with a poly(dG) tail. Upon base pairing between the poly(dG) tail of the barcode and the poly(dC) tail of the cDNA molecule, the reverse transcriptase switches template strands, from cellular RNA molecule to the barcode, and continues replication to the 5′ end of the barcode. By doing so, the resulting cDNA molecule contains the sequence of the barcode (such as the molecular label) on the 3′ end of the cDNA molecule.

Reverse transcription can occur repeatedly to produce multiple labeled-cDNA molecules. The methods disclosed herein can comprise conducting at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 reverse transcription reactions. The method can comprise conducting at least about 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 reverse transcription reactions.

Amplification

One or more nucleic acid amplification reactions (e.g., at block 228 of FIG. 2) can be performed to create multiple copies of the labeled target nucleic acid molecules. Amplification can be performed in a multiplexed manner, wherein multiple target nucleic acid sequences are amplified simultaneously. The amplification reaction can be used to add sequencing adaptors to the nucleic acid molecules. The amplification reactions can comprise amplifying at least a portion of a sample label, if present. The amplification reactions can comprise amplifying at least a portion of the cellular label and/or barcode sequence (e.g., a molecular label). The amplification reactions can comprise amplifying at least a portion of a sample tag, a cell label, a spatial label, a barcode sequence (e.g., a molecular label), a target nucleic acid, or a combination thereof. The amplification reactions can comprise amplifying 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, 100%, or a range or a number between any two of these values, of the plurality of nucleic acids. The method can further comprise conducting one or more cDNA synthesis reactions to produce one or more cDNA copies of target-barcode molecules comprising a sample label, a cell label, a spatial label, and/or a barcode sequence (e.g., a molecular label).

In some embodiments, amplification can be performed using a polymerase chain reaction (PCR). As used herein, PCR can refer to a reaction for the in vitro amplification of specific DNA sequences by the simultaneous primer extension of complementary strands of DNA. As used herein, PCR can encompass derivative forms of the reaction, including but not limited to, RT-PCR, real-time PCR, nested PCR, quantitative PCR, multiplexed PCR, digital PCR, and assembly PCR.

Amplification of the labeled nucleic acids can comprise non-PCR based methods. Examples of non-PCR based methods include, but are not limited to, multiple displacement amplification (MDA), transcription-mediated amplification (TMA), nucleic acid sequence-based amplification (NASBA), strand displacement amplification (SDA), real-time SDA, rolling circle amplification, or circle-to-circle amplification. Other non-PCR-based amplification methods include multiple cycles of DNA-dependent RNA polymerase-driven RNA transcription amplification or RNA-directed DNA synthesis and transcription to amplify DNA or RNA targets, a ligase chain reaction (LCR), and a Qβ replicase (Qβ) method, use of palindromic probes, strand displacement amplification, oligonucleotide-driven amplification using a restriction endonuclease, an amplification method in which a primer is hybridized to a nucleic acid sequence and the resulting duplex is cleaved prior to the extension reaction and amplification, strand displacement amplification using a nucleic acid polymerase lacking 5′ exonuclease activity, rolling circle amplification, and ramification extension amplification (RAM). In some embodiments, the amplification does not produce circularized transcripts.

In some embodiments, the methods disclosed herein further comprise conducting a polymerase chain reaction on the labeled nucleic acid (e.g., labeled-RNA, labeled-DNA, labeled-cDNA) to produce a labeled amplicon (e.g., a stochastically labeled amplicon). The labeled amplicon can be double-stranded molecule. The double-stranded molecule can comprise a double-stranded RNA molecule, a double-stranded DNA molecule, or a RNA molecule hybridized to a DNA molecule. One or both of the strands of the double-stranded molecule can comprise a sample label, a spatial label, a cell label, and/or a barcode sequence (e.g., a molecular label). The labeled amplicon can be a single-stranded molecule. The single-stranded molecule can comprise DNA, RNA, or a combination thereof. The nucleic acids of the disclosure can comprise synthetic or altered nucleic acids.

Amplification can comprise use of one or more non-natural nucleotides. Non-natural nucleotides can comprise photolabile or triggerable nucleotides. Examples of non-natural nucleotides can include, but are not limited to, peptide nucleic acid (PNA), morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Non-natural nucleotides can be added to one or more cycles of an amplification reaction. The addition of the non-natural nucleotides can be used to identify products as specific cycles or time points in the amplification reaction.

Conducting the one or more amplification reactions can comprise the use of one or more primers. The one or more primers can comprise, for example, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 or more nucleotides. The one or more primers can comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 or more nucleotides. The one or more primers can comprise less than 12-15 nucleotides. The one or more primers can anneal to at least a portion of the plurality of labeled targets (e.g., stochastically labeled targets). The one or more primers can anneal to the 3′ end or 5′ end of the plurality of labeled targets. The one or more primers can anneal to an internal region of the plurality of labeled targets. The internal region can be at least about 50, 100, 150, 200, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 650, 700, 750, 800, 850, 900 or 1000 nucleotides from the 3′ ends the plurality of labeled targets. The one or more primers can comprise a fixed panel of primers. The one or more primers can comprise at least one or more custom primers. The one or more primers can comprise at least one or more control primers. The one or more primers can comprise at least one or more gene-specific primers.

The one or more primers can comprise a universal primer. The universal primer can anneal to a universal primer binding site. The one or more custom primers can anneal to a first sample label, a second sample label, a spatial label, a cell label, a barcode sequence (e.g., a molecular label), a target, or any combination thereof. The one or more primers can comprise a universal primer and a custom primer. The custom primer can be designed to amplify one or more targets. The targets can comprise a subset of the total nucleic acids in one or more samples. The targets can comprise a subset of the total labeled targets in one or more samples. The one or more primers can comprise at least 96 or more custom primers. The one or more primers can comprise at least 960 or more custom primers. The one or more primers can comprise at least 9600 or more custom primers. The one or more custom primers can anneal to two or more different labeled nucleic acids. The two or more different labeled nucleic acids can correspond to one or more genes.

Any amplification scheme can be used in the methods of the present disclosure. For example, in one scheme, the first round PCR can amplify molecules attached to the bead using a gene specific primer and a primer against the universal Illumina sequencing primer 1 sequence. The second round of PCR can amplify the first PCR products using a nested gene specific primer flanked by Illumina sequencing primer 2 sequence, and a primer against the universal Illumina sequencing primer 1 sequence. The third round of PCR adds P5 and P7 and sample index to turn PCR products into an Illumina sequencing library. Sequencing using 150 bp×2 sequencing can reveal the cell label and barcode sequence (e.g., molecular label) on read 1, the gene on read 2, and the sample index on index 1 read.

In some embodiments, nucleic acids can be removed from the substrate using chemical cleavage. For example, a chemical group or a modified base present in a nucleic acid can be used to facilitate its removal from a solid support. For example, an enzyme can be used to remove a nucleic acid from a substrate. For example, a nucleic acid can be removed from a substrate through a restriction endonuclease digestion. For example, treatment of a nucleic acid containing a dUTP or ddUTP with uracil-d-glycosylase (UDG) can be used to remove a nucleic acid from a substrate. For example, a nucleic acid can be removed from a substrate using an enzyme that performs nucleotide excision, such as a base excision repair enzyme, such as an apurinic/apyrimidinic (AP) endonuclease. In some embodiments, a nucleic acid can be removed from a substrate using a photocleavable group and light. In some embodiments, a cleavable linker can be used to remove a nucleic acid from the substrate. For example, the cleavable linker can comprise at least one of biotin/avidin, biotin/streptavidin, biotin/neutravidin, Ig-protein A, a photo-labile linker, acid or base labile linker group, or an aptamer.

When the probes are gene-specific, the molecules can hybridize to the probes and be reverse transcribed and/or amplified. In some embodiments, after the nucleic acid has been synthesized (e.g., reverse transcribed), it can be amplified. Amplification can be performed in a multiplex manner, wherein multiple target nucleic acid sequences are amplified simultaneously. Amplification can add sequencing adaptors to the nucleic acid.

In some embodiments, amplification can be performed on the substrate, for example, with bridge amplification. cDNAs can be homopolymer tailed in order to generate a compatible end for bridge amplification using oligo(dT) probes on the substrate. In bridge amplification, the primer that is complementary to the 3′ end of the template nucleic acid can be the first primer of each pair that is covalently attached to the solid particle. When a sample containing the template nucleic acid is contacted with the particle and a single thermal cycle is performed, the template molecule can be annealed to the first primer and the first primer is elongated in the forward direction by addition of nucleotides to form a duplex molecule consisting of the template molecule and a newly formed DNA strand that is complementary to the template. In the heating step of the next cycle, the duplex molecule can be denatured, releasing the template molecule from the particle and leaving the complementary DNA strand attached to the particle through the first primer. In the annealing stage of the annealing and elongation step that follows, the complementary strand can hybridize to the second primer, which is complementary to a segment of the complementary strand at a location removed from the first primer. This hybridization can cause the complementary strand to form a bridge between the first and second primers secured to the first primer by a covalent bond and to the second primer by hybridization. In the elongation stage, the second primer can be elongated in the reverse direction by the addition of nucleotides in the same reaction mixture, thereby converting the bridge to a double-stranded bridge. The next cycle then begins, and the double-stranded bridge can be denatured to yield two single-stranded nucleic acid molecules, each having one end attached to the particle surface via the first and second primers, respectively, with the other end of each unattached. In the annealing and elongation step of this second cycle, each strand can hybridize to a further complementary primer, previously unused, on the same particle, to form new single-strand bridges. The two previously unused primers that are now hybridized elongate to convert the two new bridges to double-strand bridges.

The amplification reactions can comprise amplifying at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, or 100% of the plurality of nucleic acids.

Amplification of the labeled nucleic acids can comprise PCR-based methods or non-PCR based methods. Amplification of the labeled nucleic acids can comprise exponential amplification of the labeled nucleic acids. Amplification of the labeled nucleic acids can comprise linear amplification of the labeled nucleic acids. Amplification can be performed by polymerase chain reaction (PCR). PCR can refer to a reaction for the in vitro amplification of specific DNA sequences by the simultaneous primer extension of complementary strands of DNA. PCR can encompass derivative forms of the reaction, including but not limited to, RT-PCR, real-time PCR, nested PCR, quantitative PCR, multiplexed PCR, digital PCR, suppression PCR, semi-suppressive PCR and assembly PCR.

In some embodiments, amplification of the labeled nucleic acids comprises non-PCR based methods. Examples of non-PCR based methods include, but are not limited to, multiple displacement amplification (MDA), transcription-mediated amplification (TMA), nucleic acid sequence-based amplification (NASBA), strand displacement amplification (SDA), real-time SDA, rolling circle amplification, or circle-to-circle amplification. Other non-PCR-based amplification methods include multiple cycles of DNA-dependent RNA polymerase-driven RNA transcription amplification or RNA-directed DNA synthesis and transcription to amplify DNA or RNA targets, a ligase chain reaction (LCR), a Qβ replicase (Qβ), use of palindromic probes, strand displacement amplification, oligonucleotide-driven amplification using a restriction endonuclease, an amplification method in which a primer is hybridized to a nucleic acid sequence and the resulting duplex is cleaved prior to the extension reaction and amplification, strand displacement amplification using a nucleic acid polymerase lacking 5′ exonuclease activity, rolling circle amplification, and/or ramification extension amplification (RAM).

In some embodiments, the methods disclosed herein further comprise conducting a nested polymerase chain reaction on the amplified amplicon (e.g., target). The amplicon can be double-stranded molecule. The double-stranded molecule can comprise a double-stranded RNA molecule, a double-stranded DNA molecule, or a RNA molecule hybridized to a DNA molecule. One or both of the strands of the double-stranded molecule can comprise a sample tag or molecular identifier label. Alternatively, the amplicon can be a single-stranded molecule. The single-stranded molecule can comprise DNA, RNA, or a combination thereof. The nucleic acids of the present invention can comprise synthetic or altered nucleic acids.

In some embodiments, the method comprises repeatedly amplifying the labeled nucleic acid to produce multiple amplicons. The methods disclosed herein can comprise conducting at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 amplification reactions. Alternatively, the method comprises conducting at least about 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 amplification reactions.

Amplification can further comprise adding one or more control nucleic acids to one or more samples comprising a plurality of nucleic acids. Amplification can further comprise adding one or more control nucleic acids to a plurality of nucleic acids. The control nucleic acids can comprise a control label.

Amplification can comprise use of one or more non-natural nucleotides. Non-natural nucleotides can comprise photolabile and/or triggerable nucleotides. Examples of non-natural nucleotides include, but are not limited to, peptide nucleic acid (PNA), morpholine and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Non-natural nucleotides can be added to one or more cycles of an amplification reaction. The addition of the non-natural nucleotides can be used to identify products as specific cycles or time points in the amplification reaction.

Conducting the one or more amplification reactions can comprise the use of one or more primers. The one or more primers can comprise one or more oligonucleotides. The one or more oligonucleotides can comprise at least about 7-9 nucleotides. The one or more oligonucleotides can comprise less than 12-15 nucleotides. The one or more primers can anneal to at least a portion of the plurality of labeled nucleic acids. The one or more primers can anneal to the 3′ end and/or 5′ end of the plurality of labeled nucleic acids. The one or more primers can anneal to an internal region of the plurality of labeled nucleic acids. The internal region can be at least about 50, 100, 150, 200, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 650, 700, 750, 800, 850, 900 or 1000 nucleotides from the 3′ ends the plurality of labeled nucleic acids. The one or more primers can comprise a fixed panel of primers. The one or more primers can comprise at least one or more custom primers. The one or more primers can comprise at least one or more control primers. The one or more primers can comprise at least one or more housekeeping gene primers. The one or more primers can comprise a universal primer. The universal primer can anneal to a universal primer binding site. The one or more custom primers can anneal to the first sample tag, the second sample tag, the molecular identifier label, the nucleic acid or a product thereof. The one or more primers can comprise a universal primer and a custom primer. The custom primer can be designed to amplify one or more target nucleic acids. The target nucleic acids can comprise a subset of the total nucleic acids in one or more samples. In some embodiments, the primers are the probes attached to the array of the disclosure.

In some embodiments, barcoding (e.g., stochastically barcoding) the plurality of targets in the sample further comprises generating an indexed library of the barcoded targets (e.g., stochastically barcoded targets) or barcoded fragments of the targets. The barcode sequences of different barcodes (e.g., the molecular labels of different stochastic barcodes) can be different from one another. Generating an indexed library of the barcoded targets includes generating a plurality of indexed polynucleotides from the plurality of targets in the sample. For example, for an indexed library of the barcoded targets comprising a first indexed target and a second indexed target, the label region of the first indexed polynucleotide can differ from the label region of the second indexed polynucleotide by, by about, by at least, or by at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, or a number or a range between any two of these values, nucleotides. In some embodiments, generating an indexed library of the barcoded targets includes contacting a plurality of targets, for example mRNA molecules, with a plurality of oligonucleotides including a poly(T) region and a label region; and conducting a first strand synthesis using a reverse transcriptase to produce single-strand labeled cDNA molecules each comprising a cDNA region and a label region, wherein the plurality of targets includes at least two mRNA molecules of different sequences and the plurality of oligonucleotides includes at least two oligonucleotides of different sequences. Generating an indexed library of the barcoded targets can further comprise amplifying the single-strand labeled cDNA molecules to produce double-strand labeled cDNA molecules; and conducting nested PCR on the double-strand labeled cDNA molecules to produce labeled amplicons. In some embodiments, the method can include generating an adaptor-labeled amplicon.

Barcoding (e.g., stochastic barcoding) can include using nucleic acid barcodes or tags to label individual nucleic acid (e.g., DNA or RNA) molecules. In some embodiments, it involves adding DNA barcodes or tags to cDNA molecules as they are generated from mRNA. Nested PCR can be performed to minimize PCR amplification bias. Adaptors can be added for sequencing using, for example, next generation sequencing (NGS). The sequencing results can be used to determine cell labels, molecular labels, and sequences of nucleotide fragments of the one or more copies of the targets, for example at block 232 of FIG. 2.

FIG. 3 is a schematic illustration showing a non-limiting exemplary process of generating an indexed library of the barcoded targets (e.g., stochastically barcoded targets), such as barcoded mRNAs or fragments thereof. As shown in step 1, the reverse transcription process can encode each mRNA molecule with a unique molecular label sequence, a cell label sequence, and a universal PCR site. In particular, RNA molecules 302 can be reverse transcribed to produce labeled cDNA molecules 304, including a cDNA region 306, by hybridization (e.g., stochastic hybridization) of a set of barcodes (e.g., stochastic barcodes) 310 to the poly(A) tail region 308 of the RNA molecules 302. Each of the barcodes 310 can comprise a target-binding region, for example a poly(dT) region 312, a label region 314 (e.g., a barcode sequence or a molecule), and a universal PCR region 316.

In some embodiments, the cell label sequence can include 3 to 20 nucleotides. In some embodiments, the molecular label sequence can include 3 to 20 nucleotides. In some embodiments, each of the plurality of stochastic barcodes further comprises one or more of a universal label and a cell label, wherein universal labels are the same for the plurality of stochastic barcodes on the solid support and cell labels are the same for the plurality of stochastic barcodes on the solid support. In some embodiments, the universal label can include 3 to 20 nucleotides. In some embodiments, the cell label comprises 3 to 20 nucleotides.

In some embodiments, the label region 314 can include a barcode sequence or a molecular label 318 and a cell label 320. In some embodiments, the label region 314 can include one or more of a universal label, a dimension label, and a cell label. The barcode sequence or molecular label 318 can be, can be about, can be at least, or can be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, or a number or a range between any of these values, of nucleotides in length. The cell label 320 can be, can be about, can be at least, or can be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, or a number or a range between any of these values, of nucleotides in length. The universal label can be, can be about, can be at least, or can be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, or a number or a range between any of these values, of nucleotides in length. Universal labels can be the same for the plurality of stochastic barcodes on the solid support and cell labels are the same for the plurality of stochastic barcodes on the solid support. The dimension label can be, can be about, can be at least, or can be at most 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, or a number or a range between any of these values, of nucleotides in length.

In some embodiments, the label region 314 can comprise, comprise about, comprise at least, or comprise at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, or a number or a range between any of these values, different labels, such as a barcode sequence or a molecular label 318 and a cell label 320. Each label can be, can be about, can be at least, or can be at most 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, or a number or a range between any of these values, of nucleotides in length. A set of barcodes or stochastic barcodes 310 can contain, contain about, contain at least, or can be at most, 10, 20, 40, 50, 70, 80, 90, 10², 10³, 10⁴, 10⁵, 10⁶, 10⁷, 10⁸, 10⁹, 10¹⁰, 10¹¹, 10¹², 10¹³, 10¹⁴, 10¹⁵, 10²⁰, or a number or a range between any of these values, barcodes or stochastic barcodes 310. And the set of barcodes or stochastic barcodes 310 can, for example, each contain a unique label region 314. The labeled cDNA molecules 304 can be purified to remove excess barcodes or stochastic barcodes 310. Purification can comprise Ampure bead purification.

As shown in step 2, products from the reverse transcription process in step 1 can be pooled into 1 tube and PCR amplified with a 1^(st) PCR primer pool and a 1^(st) universal PCR primer. Pooling is possible because of the unique label region 314. In particular, the labeled cDNA molecules 304 can be amplified to produce nested PCR labeled amplicons 322. Amplification can comprise multiplex PCR amplification. Amplification can comprise a multiplex PCR amplification with 96 multiplex primers in a single reaction volume. In some embodiments, multiplex PCR amplification can utilize, utilize about, utilize at least, or utilize at most, 10, 20, 40, 50, 70, 80, 90, 10², 10³, 10⁴, 10⁵, 10⁶, 10⁷, 10⁸, 10⁹, 10¹⁰, 10¹¹, 10¹², 10¹³, 10¹⁴, 10¹⁵, 10²⁰, or a number or a range between any of these values, multiplex primers in a single reaction volume. Amplification can comprise using a 1^(st) PCR primer pool 324 comprising custom primers 326A-C targeting specific genes and a universal primer 328. The custom primers 326 can hybridize to a region within the cDNA portion 306′ of the labeled cDNA molecule 304. The universal primer 328 can hybridize to the universal PCR region 316 of the labeled cDNA molecule 304.

As shown in step 3 of FIG. 3, products from PCR amplification in step 2 can be amplified with a nested PCR primers pool and a 2^(nd) universal PCR primer. Nested PCR can minimize PCR amplification bias. In particular, the nested PCR labeled amplicons 322 can be further amplified by nested PCR. The nested PCR can comprise multiplex PCR with nested PCR primers pool 330 of nested PCR primers 332 a-c and a 2^(nd) universal PCR primer 328′ in a single reaction volume. The nested PCR primer pool 328 can contain, contain about, contain at least, or contain at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, or a number or a range between any of these values, different nested PCR primers 330. The nested PCR primers 332 can contain an adaptor 334 and hybridize to a region within the cDNA portion 306″ of the labeled amplicon 322. The universal primer 328′ can contain an adaptor 336 and hybridize to the universal PCR region 316 of the labeled amplicon 322. Thus, step 3 produces adaptor-labeled amplicon 338. In some embodiments, nested PCR primers 332 and the 2^(nd) universal PCR primer 328′ may not contain the adaptors 334 and 336. The adaptors 334 and 336 can instead be ligated to the products of nested PCR to produce adaptor-labeled amplicon 338.

As shown in step 4, PCR products from step 3 can be PCR amplified for sequencing using library amplification primers. In particular, the adaptors 334 and 336 can be used to conduct one or more additional assays on the adaptor-labeled amplicon 338. The adaptors 334 and 336 can be hybridized to primers 340 and 342. The one or more primers 340 and 342 can be PCR amplification primers. The one or more primers 340 and 342 can be sequencing primers. The one or more adaptors 334 and 336 can be used for further amplification of the adaptor-labeled amplicons 338. The one or more adaptors 334 and 336 can be used for sequencing the adaptor-labeled amplicon 338. The primer 342 can contain a plate index 344 so that amplicons generated using the same set of barcodes or stochastic barcodes 310 can be sequenced in one sequencing reaction using next generation sequencing (NGS).

Hybrid Library Preparation

In recent years, next generation sequencing (NGS) has provided a high throughput method in assessing gene expression profiles. During library preparation for NGS, a sample with heterogeneous cDNA species is amplified by PCR to obtain adequate sample amount and to attach NGS-compatible adapters. The sequencing process captures the number of reads for each gene from the PCR-amplified library sample to interpret the gene expression level. The level of gene expression in a biological sample can vary greatly. For examples, it has been described that gene expression level follows 3 broad categories: 1) ‘high expressers,’ which are comprised of 5-10 genes that dominate ˜20% of cellular mRNAs; 2) ‘intermediate expressers’ that are comprised of 50-200 genes that occupy 40-60% of cellular mRNAs; and 3) ‘moderate expressers’ that are comprised of 10,000-20,000 genes that occupy the rest of the cellular mRNA fraction. One challenge in molecular biology and molecular genetics is to be able to capture this highly dynamic gene expression profile efficiently and effectively in order to distinguish different cell types and phenotypes in the sample. As different genes are expressed at a large range of levels, PCR amplification can skew the native gene expression. For example, a gene has 1 molecule of cDNA would require 40 cycles of PCR to achieve the same representative amount as a gene with 1000 molecules of cDNA in 30 cycles. In a heterogeneous cDNA sample, PCR is usually performed in excess cycles to adequately amplify low expressers; in those scenarios, the native gene expression profile is usually skewed by the dominating high expresser PCR products. A method to correct for such bias in PCR product is Molecular Indexing; however, high expressers such as ribosomal protein mRNAs, mitochondrial mRNAs, or housekeeping genes often dominate the sequencing run with little contribution to the experimental interpretation, rendering the sequencing cost for Molecular Index counting to be expensive.

Previous efforts to increase the relative abundance of low abundance species in a nucleic acid sample have included library normalization methods. One library normalization method or strategy includes hybridizing the library to another set of nucleic acids where the sequences are uniformly represented, such as the genomic DNA from the source organism, and retaining the hybridized fraction. Another library normalization method or strategy is based on the concentration dependence of solution hybridization (e.g., when a set of dsDNA molecules are denatured, they will rehybridize at a rate proportional to the square of their original concentrations). Exemplary library normalization methods are described in U.S. Patent Application Publications US 2017/0073730 and 2017/0342484; the content of each of which is incorporated herein by reference in its entirety. The methods, compositions and kits disclosed herein, in some embodiments, can complement or supplement these library normalization strategies. The methods, compositions and kits disclosed herein, in some embodiments, can avoid the use of physical and enzymatic separation of ssDNA and dsDNA fractions during library normalization. The methods, compositions and kits disclosed herein, in some embodiments, can avoid the need to hybridize a library to another set of nucleic acids. Disclosed herein include systems and methods of increasing the relative abundance of low abundance species within a sample by hybrid library preparation (e.g., hybrid cDNA library preparation and/or hybrid sequencing library preparation).

As single-cell molecular-indexed whole transcriptome amplification (WTA) analysis does not employ target-specific primers during library amplification, this assay provides the advantage of being able to perform discovery biology. The performance of scRNA-seq with a panel of target-specific multiplex primers has greater sensitivity relative to WTA analysis (particularly for low abundance targets), but this approach cannot target the entire transcriptome and thus cannot be employed for discovery biology. The methods disclosed herein bridges the advantages of both WTA and target-specific panel scRNA-seq approaches, combining the use of target-specific primers and target non-specific primers during the preparation of cDNA libraries and/or sequencing libraries. The methods disclosed herein can yield increased sensitivity of low abundance transcripts, detection of new transcripts, detection of new cell types, and/or reduced sequencing costs

The methods, compositions and kits disclosed herein, in some embodiments, increase the abundance of select low abundance targets during cDNA library preparation and/or sequencing library preparation. In some embodiments, increasing the abundance of select low abundance targets during cDNA library preparation and/or sequencing library preparation comprises hybrid library preparation (e.g., hybrid cDNA library preparation, hybrid sequencing library preparation). In some embodiments, hybrid cDNA library preparation comprises: (a) whole transcriptome cDNA amplification with non-target-specific primers (e.g., random primers, WTA primers, primers annealing to a universal sequence) and (b) target-specific cDNA amplification with target-specific primers. In some embodiments, hybrid sequencing library preparation comprises: (a) extension and/or amplification using non-target-specific primers (e.g., random primers, WTA primers, primers annealing to a universal sequence); and (b) extension and/or amplification using target-specific primers. In some embodiments, the methods comprise the use of non-target-specific primers (e.g., WTA primers) and a panel of target-specific primers during cDNA library preparation. In some embodiments, the methods comprise the use of non-target-specific primers and a panel of target-specific primers during sequencing library preparation. In some embodiments, the methods comprise the use of non-target-specific primers and a panel of target-specific primers during both cDNA library preparation and sequencing library preparation.

Some embodiments disclosed herein provide methods of increasing the relative abundance of one or more low abundance species during cDNA library preparation and/or hybrid sequencing library preparation. As used herein, a “target” can be used interchangeably with a “species.” In some embodiments, the method of increasing the relative abundance of one or more low abundance species comprises methods of hybrid cDNA library preparation and/or hybrid sequencing library preparation as disclosed herein. In some embodiments, increasing the relative abundance of one or more low abundance species comprises performing cDNA library preparation with both WTA (e.g., random) primers and target-specific primers. In some embodiments, increasing the relative abundance of one or more low abundance species comprises performing sequencing library preparation with both non-target-specific primers and target-specific primers.

As used herein, a “species” refers to the polynucleotides (for example, single-stranded polynucleotides, such as cDNA molecules, sample indexing oligonucleotides for sample tracking, and protein-specific oligonucleotides for determining protein expression profiles) that are the same, the complement, or the reverse complement of one another, or are capable of hybridize to one another, or are transcripts from the same genetic locus, or encode the same protein or fragment thereof, etc. As used herein, “target” can be used interchangeably with “species.” In some embodiments, members (e.g., copies or occurrences) of a species are at least 80%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% homologous to one another, or complement thereof. In some embodiments, members of a species are transcripts from the same genetic locus and the transcripts can be of the same or different lengths. The species is, in some embodiments, cDNA or mRNA.

As used herein, a “high abundance species” refers to a species that is present in high amount, for example the species can be, be about, be at least, or be at least about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 20%, 30%, 40%, 50%, or more. In some embodiments, a sample can comprise at least 1, at least 2, at least 3, at least 4, at least 5, at least 10, at least 20, at least 50, at least 100, at least 200, at least 500, at least 1,000, or more, high abundance species. In some embodiments, the total of all the high abundance species represents at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or more of the nucleic acid molecules or species in the sample. In some embodiments, high abundance species can comprise polynucleotides encoding one or more ribosomal proteins. In some embodiments, high abundance species can comprise polynucleotides encoding one or more mitochondrial proteins. In some embodiments, high abundance species can comprise polynucleotides encoding one or more housekeeping proteins. In some embodiments, the high abundance species can comprise the sequence of a sample indexing oligonucleotide (also referred to herein as sample tag sequences or sample indexing indices). In some embodiments, the high abundance species can comprise the sequence of an oligonucleotide used for sample tracking. In some embodiments, the high abundance species can comprise a sample indexing sequence for identifying sample origin of one or more cells of a sample. In some embodiments, the high abundance species can comprise a unique identifier sequence for a cellular component binding reagent (e.g., an antibody). In some embodiments, the high abundance species can comprise the sequence of an oligonucleotide conjugated (or previously conjugated) to a cellular component binding reagent. In some embodiments, the high abundance species can comprise the sequence of an oligonucleotide conjugated or previously conjugated with an antibody can be referred to herein as an antibody oligonucleotide (abbreviated as an “AbOligo” or “AbO”).

As used herein, an “intermediate abundance species” refers to a species that is present in an amount that is lower than at least one species and is higher than at least one other species. In some embodiments, an intermediate abundance species can be, be at least, be about, or be at least about 10%, 5%, 4%, 3%, 2%, 1%, 0.1%, 0.01%, or a range between any two of the above values, of the nucleic acid molecules or species in the sample. In some embodiments, the sample can comprise at least 1, at least 2, at least 3, at least 4, at least 5, at least 10, at least 20, at least 50, at least 100, at least 200, at least 500, at least 1,000, or more, intermediate abundance species. In some embodiments, the total of all the intermediate abundance species represents about 1%, about 2%, about 3%, about 4%, about 5%, about 10%, about 20%, about 30%, or a range between any two of the above values, of the nucleic acid molecules in the sample. In some embodiments, intermediate abundance species can comprise polynucleotides encoding one or more housekeeping proteins. In some embodiments, the intermediate abundance species can comprise the sequence of an oligonucleotide used for sample tracking. In some embodiments, the intermediate abundance species can comprise a sample indexing sequence for identifying sample origin of one or more cells of a sample. In some embodiments, the intermediate abundance species can comprise a unique identifier sequence for a cellular component binding reagent (e.g., an antibody). In some embodiments, the intermediate abundance species can comprise the sequence of an oligonucleotide conjugated (or previously conjugated) to a cellular component binding reagent. In some embodiments, the intermediate abundance species can comprise the sequence of an oligonucleotide conjugated or previously conjugated with an antibody can be referred to herein as an antibody oligonucleotide (abbreviated as an “AbOligo” or “AbO”).

As used herein, a “low abundance species” refers to a species that is present in low amount, for example the species can be, be about, be less than, or be less than about, 1%, 0.1%, 0.01%, 0.001%, 0.0001%, or less of the nucleic acid molecules or species in the sample. In some embodiments, the sample can comprise at least 1, at least 2, at least 3, at least 4, at least 5, at least 10, at least 20, at least 50, at least 100, at least 200, at least 500, at least 1,000, or more, low abundance species. In some embodiments, the total of all the low abundance species represents less than 20%, less than 10%, less than 5%, less than 4%, less than 3%, less than 2%, less than 1%, less than 0.1%, or less of the nucleic acid molecules in the sample. In some embodiments, low abundance species can comprise polynucleotides encoding one or more transcription factors. In some embodiments, low abundance species can comprise polynucleotides encoding one or more T cell receptors. In some embodiments, low abundance species can comprise polynucleotides encoding one or more antibodies.

In some embodiments, two or more of the plurality of nucleic acid targets comprises low abundance species. In some embodiments, two or more of the plurality of nucleic acid targets comprises low abundance and/or intermediate abundance species. In some embodiments, two or more of the plurality of nucleic acid targets comprises mRNAs of a low-expressing gene. In some embodiments, the percentage of nucleic acid targets that represent low abundance species can be, or be about, 0.000000001%, 0.00000001%, 0.0000001%, 0.000001%, 0.00001%, 0.0001%, 0.001%, 0.01%, 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%, or a number or a range between any two of these values. In some embodiments, the percentage of nucleic acid targets that represent low abundance species can be at least, or at most, 0.000000001%, 0.00000001%, 0.0000001%, 0.000001%, 0.00001%, 0.0001%, 0.001%, 0.01%, 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%.

In some embodiments, a hybrid library comprises the products of hybrid cDNA library preparation and/or sequencing cDNA library preparation. In some embodiments, a hybrid library comprises amplicons derived from hybrid cDNA library preparation and/or sequencing cDNA library preparation. In some embodiments, a hybrid library comprises the products of one or more downstream methods of the disclosure employing the products of hybrid cDNA library preparation and/or sequencing cDNA library preparation as templates. In some embodiments, sequencing of a hybrid library generated by the methods of the disclosure yields an increase in the number of reads of one or more low abundance species (e.g., a nucleic acid target, a low-expressed mRNA) as compared to the sequencing of an non-hybrid library. In some embodiments, the increase in the number of reads of one or more low abundance species in the hybrid library is at least 2% (e.g., 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 40%, 50%, 75%, 100%, or higher and overlapping ranges therein) as compared to the non-hybrid library. In some embodiments, sequencing of a hybrid library generated by the methods of the disclosure yields an increase in the number of reads of one or more low abundance species (e.g., a nucleic acid target, a low-expressed mRNA) relative to the total number of reads as compared to the sequencing of an non-hybrid library. In some embodiments, the increase in the number of reads of one or more low abundance species relative to the total number of reads in the hybrid library is at least 2% (e.g., 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 40%, 50%, 75%, 100%, or higher and overlapping ranges therein) as compared to the non-hybrid library. In some embodiments, sequencing of a hybrid library generated by the methods of the disclosure yields an increase in the number of molecular indexes (MIs) of one or more low abundance species (e.g., a nucleic acid target, a low-expressed mRNA) compared to the sequencing of an non-hybrid library. In some embodiments, the increase in the number of MIs of one or more low abundance species relative to the total number of reads in the hybrid library is at least 2% (e.g., 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 40%, 50%, 75%, 100%, or higher and overlapping ranges therein) as compared to the non-hybrid library.

In some embodiments, the methods disclosed herein can increase the relative abundance of one or more low abundance species after hybrid cDNA library preparation and/or hybrid sequencing library preparation (compared to non-hybrid cDNA library preparation and/or non-hybrid sequencing library preparation). For example, the methods disclosed herein can increase the relative abundance of at least 1, at least 2, at least 3, at least 4, at least 5, at least 10, at least 20, at least 50, at least 100, at least 200, at least 500, at least 1,000, or more, low abundance species after hybrid cDNA library preparation and/or hybrid sequencing library preparation (compared to non-hybrid cDNA library preparation and/or non-hybrid sequencing library preparation). In some embodiments, the methods disclosed herein can increase the relative abundance after hybrid cDNA library preparation and/or hybrid sequencing library preparation (compared to non-hybrid cDNA library preparation and/or non-hybrid sequencing library preparation) by at least about 2% (e.g., 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 40%, 50%, 75%, 100%, 150%, 200%, 250%, 500%, 1000%, or higher and overlapping ranges therein) of each of the one or more low abundance species. In some embodiments, the methods disclosed herein can increase the relative abundance by at least about 2% (e.g., 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 40%, 50%, 75%, 100%, 150%, 200%, 250%, 500%, 1000%, or higher and overlapping ranges therein) of at least one of the one or more low abundance species after hybrid cDNA library preparation and/or hybrid sequencing library preparation (compared to non-hybrid cDNA library preparation and/or non-hybrid sequencing library preparation). In some embodiments, the methods disclosed herein can increase the relative abundance of the total low abundance species after hybrid cDNA library preparation and/or hybrid sequencing library preparation (compared to non-hybrid cDNA library preparation and/or non-hybrid sequencing library preparation) by at least about 2% (e.g., 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 40%, 50%, 75%, 100%, 150%, 200%, 250%, 500%, 1000%, or higher and overlapping ranges therein).

In some embodiments, the methods disclosed herein can selectively increase the relative abundance of one or more low abundance species after hybrid cDNA library preparation and/or hybrid sequencing library preparation (compared to non-hybrid cDNA library preparation and/or non-hybrid sequencing library preparation) such that the abundance of the low abundance species relative to the one or more high abundance species increases after hybrid cDNA library preparation and/or hybrid sequencing library preparation. In some embodiments, the methods and compositions disclosed herein can increase the relative abundance by, by about, by at least, or at least about 2% (e.g., 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 40%, 50%, 75%, 100%, 150%, 200%, 250%, 500%, 1000%, or higher and overlapping ranges therein) of each of the one or more low abundance species after hybrid cDNA library preparation and/or hybrid sequencing library preparation (compared to non-hybrid cDNA library preparation and/or non-hybrid sequencing library preparation) such that the abundance of the low abundance species relative to the one or more high abundance species increases after hybrid cDNA library preparation and/or hybrid sequencing library preparation.

In some embodiments, sequencing of a hybrid library generated by the methods of the disclosure yields a similar gene expression profile and/or protein expression profile (e.g., gene expression panel results) of one or more targets as compared to the sequencing of a non-hybrid library. In some embodiments, the difference in the gene expression profile and/or protein expression profile of one or more targets between the hybrid and non-hybrid library can be at most 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, or a number or a range between any of these values. In some embodiments, the R² correlation between expression profile of the hybrid and non-hybrid libraries can be 0.6, 0.7, 0.8, 0.9, 0.990, 0.999, 1.0, and overlapping ranges therein. Methods of testing correlation between expression profiles of libraries results are well understood in the art (e.g., overlay of tSNE plots).

It would be appreciated the hybrid libraries can increase the efficiency of sequencing, by increasing the sequencing reads for intermediate or low abundance species in the hybrid library, and/or decreasing the sequencing reads for high abundance species in the non-hybrid library. In the hybrid library generated by methods of the disclosure, less abundant (e.g., rarer) nucleic acid targets can be identified more easily than in a non-hybrid library. Sequencing reads of less abundant targets in a hybrid library can comprise a larger portion of total reads of than in a non-hybrid library. Sequencing reads of one or more less abundant targets in a hybrid library can comprise at least 50, 100, 150, 200, 250, 300, 350, 400, 450 or 500% or more reads compared to reads of the same target in an non-hybrid library. Sequencing reads of one or more less abundant targets in a normalized library can be at least 1, 2, 3, 4, 5, or 6 or more fold(s) than sequencing reads for the same target in an non-hybrid library.

The methods described herein for producing hybrid libraries can improve the sequencing reads for intermediate and/or low abundance nucleic acid targets in a plurality of nucleic acids (e.g., a nucleic acid library). For example, the sequencing reads for the plurality of intermediate abundance nucleic acid targets can be at least 30%, at least 20%, at least 10%, at least 5%, of the total sequencing reads of the hybrid library. In some embodiments, the sequencing reads for the plurality of low abundance nucleic acid targets can be at least 50%, at least 40%, at least 30%, at least 20%, at least 10%, at least 5%, at least 4%, at least 3%, at least 2%, at least 1%, of the total sequencing reads of the hybrid library. In some embodiments, the sequencing reads for the plurality of low abundance nucleic acid targets is at least 5% of the total sequencing reads of the hybrid library. In some embodiments, the sequencing reads for the plurality of low abundance nucleic acid targets is at least 10% of the total sequencing reads of the hybrid library. In some embodiments, the sequencing reads for the plurality of low abundance nucleic acid targets is at least 20% of the total sequencing reads of the hybrid library. In some embodiments, the sequencing reads for the plurality of low abundance nucleic acid targets is at least 30% of the total sequencing reads of the hybrid library. In some embodiments, the sequencing reads for the plurality of low abundance nucleic acid targets is at least 40% of the total sequencing reads of the hybrid library. In some embodiments, the sequencing reads for the plurality of low abundance nucleic acid targets is at least 50% of the total sequencing reads of the hybrid library.

In some embodiments, hybrid cDNA library preparation comprises: (a) whole transcriptome cDNA amplification with non-target-specific primers (e.g., random primers, WTA primers, primers annealing to a universal sequence), generating a first plurality of barcoded amplicons (e.g., first plurality of double-stranded barcoded polynucleotides); and (b) target-specific cDNA amplification with target-specific primers, generating a second plurality of barcoded amplicons (e.g., first plurality of double-stranded barcoded polynucleotides). In some embodiments, hybrid sequencing library preparation comprises: (a) extension and/or amplification using non-target-specific primers (e.g., random primers, primers annealing to a universal sequence), generating a first plurality of barcoded amplicons; and (b) extension and/or amplification using target-specific primers, generating a second plurality of barcoded amplicons.

In some embodiments, the ratio of non-target-specific primers to target-specific primers ranges from 1:100 to 100:1. In some embodiments, the ratio of non-target-specific primers to target-specific primers is at most 10:1. In some embodiments, the ratio of non-target-specific primers to target-specific primers is at most 100:1. In some embodiments, the ratio of non-target-specific primers to target-specific primers is at most 1:1000. In some embodiments, the ratio of non-target-specific primers to target-specific primers is at least 1:10. In some embodiments, the ratio of non-target-specific primers to target-specific primers is at least 1:100. In some embodiments, the ratio of non-target-specific primers to target-specific primers is at least 1:1000.

In some embodiments, the ratio of non-target-specific primers to target-specific primers can be, or be about, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, 1:2.5, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:21, 1:22, 1:23, 1:24, 1:25, 1:26, 1:27, 1:28, 1:29, 1:30, 1:31, 1:32, 1:33, 1:34, 1:35, 1:36, 1:37, 1:38, 1:39, 1:40, 1:41, 1:42, 1:43, 1:44, 1:45, 1:46, 1:47, 1:48, 1:49, 1:50, 1:51, 1:52, 1:53, 1:54, 1:55, 1:56, 1:57, 1:58, 1:59, 1:60, 1:61, 1:62, 1:63, 1:64, 1:65, 1:66, 1:67, 1:68, 1:69, 1:70, 1:71, 1:72, 1:73, 1:74, 1:75, 1:76, 1:77, 1:78, 1:79, 1:80, 1:81, 1:82, 1:83, 1:84, 1:85, 1:86, 1:87, 1:88, 1:89, 1:90, 1:91, 1:92, 1:93, 1:94, 1:95, 1:96, 1:97, 1:98, 1:99, 1:100, 1:200, 1:300, 1:400, 1:500, 1:600, 1:700, 1:800, 1:900, 1:1000, 1:2000, 1:3000, 1:4000, 1:5000, 1:6000, 1:7000, 1:8000, 1:9000, 1:10000, or a number or a range between any two of the values. In some embodiments, the ratio of non-target-specific primers to target-specific primers can be at least, or be at most, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, 1:2.5, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:21, 1:22, 1:23, 1:24, 1:25, 1:26, 1:27, 1:28, 1:29, 1:30, 1:31, 1:32, 1:33, 1:34, 1:35, 1:36, 1:37, 1:38, 1:39, 1:40, 1:41, 1:42, 1:43, 1:44, 1:45, 1:46, 1:47, 1:48, 1:49, 1:50, 1:51, 1:52, 1:53, 1:54, 1:55, 1:56, 1:57, 1:58, 1:59, 1:60, 1:61, 1:62, 1:63, 1:64, 1:65, 1:66, 1:67, 1:68, 1:69, 1:70, 1:71, 1:72, 1:73, 1:74, 1:75, 1:76, 1:77, 1:78, 1:79, 1:80, 1:81, 1:82, 1:83, 1:84, 1:85, 1:86, 1:87, 1:88, 1:89, 1:90, 1:91, 1:92, 1:93, 1:94, 1:95, 1:96, 1:97, 1:98, 1:99, 1:100, 1:200, 1:300, 1:400, 1:500, 1:600, 1:700, 1:800, 1:900, 1:1000, 1:2000, 1:3000, 1:4000, 1:5000, 1:6000, 1:7000, 1:8000, 1:9000, or 1:10000.

In some embodiments, the ratio of non-target-specific primers to target-specific primers can be, or be about, 1:1, 1.1:1, 1.2:1, 1.3:1, 1.4:1, 1.5:1, 1.6:1, 1.7:1, 1.8:1, 1.9:1, 2:1, 2.5:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1, 31:1, 32:1, 33:1, 34:1, 35:1, 36:1, 37:1, 38:1, 39:1, 40:1, 41:1, 42:1, 43:1, 44:1, 45:1, 46:1, 47:1, 48:1, 49:1, 50:1, 51:1, 52:1, 53:1, 54:1, 55:1, 56:1, 57:1, 58:1, 59:1, 60:1, 61:1, 62:1, 63:1, 64:1, 65:1, 66:1, 67:1, 68:1, 69:1, 70:1, 71:1, 72:1, 73:1, 74:1, 75:1, 76:1, 77:1, 78:1, 79:1, 80:1, 81:1, 82:1, 83:1, 84:1, 85:1, 86:1, 87:1, 88:1, 89:1, 90:1, 91:1, 92:1, 93:1, 94:1, 95:1, 96:1, 97:1, 98:1, 99:1, 100:1, 200:1, 300:1, 400:1, 500:1, 600:1, 700:1, 800:1, 900:1, 1000:1, 2000:1, 3000:1, 4000:1, 5000:1, 6000:1, 7000:1, 8000:1, 9000:1, 10000:1, or a number or a range between any two of the values. In some embodiments, the ratio of non-target-specific primers to target-specific primers can be at least, or be at most, 1:1, 1.1:1, 1.2:1, 1.3:1, 1.4:1, 1.5:1, 1.6:1, 1.7:1, 1.8:1, 1.9:1, 2:1, 2.5:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1, 31:1, 32:1, 33:1, 34:1, 35:1, 36:1, 37:1, 38:1, 39:1, 40:1, 41:1, 42:1, 43:1, 44:1, 45:1, 46:1, 47:1, 48:1, 49:1, 50:1, 51:1, 52:1, 53:1, 54:1, 55:1, 56:1, 57:1, 58:1, 59:1, 60:1, 61:1, 62:1, 63:1, 64:1, 65:1, 66:1, 67:1, 68:1, 69:1, 70:1, 71:1, 72:1, 73:1, 74:1, 75:1, 76:1, 77:1, 78:1, 79:1, 80:1, 81:1, 82:1, 83:1, 84:1, 85:1, 86:1, 87:1, 88:1, 89:1, 90:1, 91:1, 92:1, 93:1, 94:1, 95:1, 96:1, 97:1, 98:1, 99:1, 100:1, 200:1, 300:1, 400:1, 500:1, 600:1, 700:1, 800:1, 900:1, 1000:1, 2000:1, 3000:1, 4000:1, 5000:1, 6000:1, 7000:1, 8000:1, 9000:1, or 10000:1.

In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons ranges from 1:100 to 100:1. In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons is at most 10:1. In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons is at most 100:1. In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons is at most 1:1000. In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons is at least 1:10. In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons is at least 1:100. In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons is at least 1:1000.

In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons can be, or be about, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, 1:2.5, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:21, 1:22, 1:23, 1:24, 1:25, 1:26, 1:27, 1:28, 1:29, 1:30, 1:31, 1:32, 1:33, 1:34, 1:35, 1:36, 1:37, 1:38, 1:39, 1:40, 1:41, 1:42, 1:43, 1:44, 1:45, 1:46, 1:47, 1:48, 1:49, 1:50, 1:51, 1:52, 1:53, 1:54, 1:55, 1:56, 1:57, 1:58, 1:59, 1:60, 1:61, 1:62, 1:63, 1:64, 1:65, 1:66, 1:67, 1:68, 1:69, 1:70, 1:71, 1:72, 1:73, 1:74, 1:75, 1:76, 1:77, 1:78, 1:79, 1:80, 1:81, 1:82, 1:83, 1:84, 1:85, 1:86, 1:87, 1:88, 1:89, 1:90, 1:91, 1:92, 1:93, 1:94, 1:95, 1:96, 1:97, 1:98, 1:99, 1:100, 1:200, 1:300, 1:400, 1:500, 1:600, 1:700, 1:800, 1:900, 1:1000, 1:2000, 1:3000, 1:4000, 1:5000, 1:6000, 1:7000, 1:8000, 1:9000, 1:10000, or a number or a range between any two of the values. In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons can be at least, or be at most, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, 1:2.5, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:21, 1:22, 1:23, 1:24, 1:25, 1:26, 1:27, 1:28, 1:29, 1:30, 1:31, 1:32, 1:33, 1:34, 1:35, 1:36, 1:37, 1:38, 1:39, 1:40, 1:41, 1:42, 1:43, 1:44, 1:45, 1:46, 1:47, 1:48, 1:49, 1:50, 1:51, 1:52, 1:53, 1:54, 1:55, 1:56, 1:57, 1:58, 1:59, 1:60, 1:61, 1:62, 1:63, 1:64, 1:65, 1:66, 1:67, 1:68, 1:69, 1:70, 1:71, 1:72, 1:73, 1:74, 1:75, 1:76, 1:77, 1:78, 1:79, 1:80, 1:81, 1:82, 1:83, 1:84, 1:85, 1:86, 1:87, 1:88, 1:89, 1:90, 1:91, 1:92, 1:93, 1:94, 1:95, 1:96, 1:97, 1:98, 1:99, 1:100, 1:200, 1:300, 1:400, 1:500, 1:600, 1:700, 1:800, 1:900, 1:1000, 1:2000, 1:3000, 1:4000, 1:5000, 1:6000, 1:7000, 1:8000, 1:9000, or 1:10000.

In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons can be, or be about, 1:1, 1.1:1, 1.2:1, 1.3:1, 1.4:1, 1.5:1, 1.6:1, 1.7:1, 1.8:1, 1.9:1, 2:1, 2.5:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1, 31:1, 32:1, 33:1, 34:1, 35:1, 36:1, 37:1, 38:1, 39:1, 40:1, 41:1, 42:1, 43:1, 44:1, 45:1, 46:1, 47:1, 48:1, 49:1, 50:1, 51:1, 52:1, 53:1, 54:1, 55:1, 56:1, 57:1, 58:1, 59:1, 60:1, 61:1, 62:1, 63:1, 64:1, 65:1, 66:1, 67:1, 68:1, 69:1, 70:1, 71:1, 72:1, 73:1, 74:1, 75:1, 76:1, 77:1, 78:1, 79:1, 80:1, 81:1, 82:1, 83:1, 84:1, 85:1, 86:1, 87:1, 88:1, 89:1, 90:1, 91:1, 92:1, 93:1, 94:1, 95:1, 96:1, 97:1, 98:1, 99:1, 100:1, 200:1, 300:1, 400:1, 500:1, 600:1, 700:1, 800:1, 900:1, 1000:1, 2000:1, 3000:1, 4000:1, 5000:1, 6000:1, 7000:1, 8000:1, 9000:1, 10000:1, or a number or a range between any two of the values. In some embodiments, the ratio of the number of the first plurality of barcoded amplicons and the number of the second plurality amplicons can be at least, or be at most, 1:1, 1.1:1, 1.2:1, 1.3:1, 1.4:1, 1.5:1, 1.6:1, 1.7:1, 1.8:1, 1.9:1, 2:1, 2.5:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1, 31:1, 32:1, 33:1, 34:1, 35:1, 36:1, 37:1, 38:1, 39:1, 40:1, 41:1, 42:1, 43:1, 44:1, 45:1, 46:1, 47:1, 48:1, 49:1, 50:1, 51:1, 52:1, 53:1, 54:1, 55:1, 56:1, 57:1, 58:1, 59:1, 60:1, 61:1, 62:1, 63:1, 64:1, 65:1, 66:1, 67:1, 68:1, 69:1, 70:1, 71:1, 72:1, 73:1, 74:1, 75:1, 76:1, 77:1, 78:1, 79:1, 80:1, 81:1, 82:1, 83:1, 84:1, 85:1, 86:1, 87:1, 88:1, 89:1, 90:1, 91:1, 92:1, 93:1, 94:1, 95:1, 96:1, 97:1, 98:1, 99:1, 100:1, 200:1, 300:1, 400:1, 500:1, 600:1, 700:1, 800:1, 900:1, 1000:1, 2000:1, 3000:1, 4000:1, 5000:1, 6000:1, 7000:1, 8000:1, 9000:1, or 10000:1.

In some embodiments, the one or more nucleic acid targets comprises a panel of target genes. In some embodiments, the one or more target-specific primers comprises a panel of target-specific multiplex primers. The one or more primers for multiplexed PCR can comprise a first gene specific primer and a nested gene-specific primer designed to anneal downstream of the first gene specific primer. The number of distinct target-specific primers within a panel of target-specific multiplex primers can be different in different implementations. In some embodiments, the number of distinct target-specific primers within a panel of target-specific multiplex primers can be, or be about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1250, 1500, 2000, 3000, or a number or a range between any two of these values. In some embodiments, the number of distinct target-specific primers within a panel of target-specific multiplex primers can be at least, or be at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1250, 1500, 2000, or 3000.

In some embodiments, the methods comprise performing non-hybrid cDNA library preparation followed by hybrid sequencing library preparation. In some embodiments, the methods comprise performing hybrid cDNA library preparation followed by non-hybrid sequencing library preparation. In some embodiments, the methods comprise performing hybrid cDNA library preparation followed by hybrid sequencing library preparation. In embodiments wherein both hybrid cDNA library preparation and hybrid sequencing library preparation are performed, the target-specific cDNA amplification primers and target-specific sequencing library primers can be directed to the same set of nucleic acid targets (e.g., anneal to the same set of transcripts). In some such embodiments, the target-specific cDNA amplification primers and target-specific sequencing library primers can anneal to the same region of the same nucleic acid target or can anneal to different regions of the same nucleic acid target. In embodiments wherein both hybrid cDNA library preparation and hybrid sequencing library preparation are performed, the target-specific cDNA amplification primers and target-specific sequencing library primers can be directed to a different set of nucleic acid targets (e.g., anneal to one or more different transcripts).

The nucleic acid targets (e.g., low abundance transcripts) of the target-specific primers during hybrid cDNA library preparation and hybrid sequencing library preparation can be identical. The one or more of the nucleic acid targets (e.g., low abundance transcripts) of the target-specific primers during hybrid cDNA library preparation and hybrid sequencing library preparation can different. In some embodiments, one or more of the nucleic acid targets is selectively extended and/or amplified by a target specific primer during hybrid cDNA library preparation is selectively extended and/or amplified by a target specific primer during hybrid sequencing library preparation. In some embodiments, one or more of the nucleic acid targets is selectively extended and/or amplified by a target specific primer during hybrid cDNA library preparation and is not selectively extended and/or amplified by a target specific primer during hybrid sequencing library preparation. In some embodiments, one or more of the nucleic acid targets is not selectively extended and/or amplified by a target specific primer during hybrid cDNA library preparation and is selectively extended and/or amplified by a target specific primer during hybrid sequencing library preparation. In some embodiments, one or more of the nucleic acid targets (e.g., low abundance transcripts) selectively extended and/or amplified by multiple target specific primer during hybrid cDNA library preparation and/or hybrid sequencing library preparation (e.g., target specific primers amplifying alternative transcripts of the same gene).

The number of amplification cycles during hybrid cDNA library preparation and/or hybrid sequencing library preparation can be different in different implementations. In some embodiments, the methods disclosed herein enable the use of fewer cycles of PCR during cDNA library preparation and/or sequencing library preparation to achieve adequate amplification of low abundance species (e.g., low expressers). In some embodiments, the number of amplification cycles during hybrid cDNA library preparation can be, or be about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, or a number or a range between any two of these values, fewer than the number of amplification cycles during non-hybrid cDNA library preparation. In some embodiments, the number of amplification cycles during hybrid sequencing library preparation can be, or be about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, or a number or a range between any two of these values, fewer than the number of amplification cycles during non-hybrid sequencing library preparation.

In some embodiments, the sample comprises an unnormalized nucleic acid library, a partially normalized nucleic acid library, or a nucleic acid library that has been normalized by other methods, such as a cDNA library, a genomic DNA library, or the like. In some embodiments, the sample can comprise a pooled unnormalized nucleic acid library, such as a pooled unnormalized nucleic acid library constructed from a plurality of unnormalized nucleic acid libraries each representing a single cell. In some embodiments, the unnormalized nucleic acid library is a cDNA library. In some embodiments, the unnormalized nucleic acid library is a genomic library. The methods, compositions and kits disclosed herein, in some embodiments, can complement or supplement library normalization strategies known in the art. Exemplary library normalization methods are described in U.S. Patent Application Publications US 2017/0073730 and 2017/0342484; the content of each of which is incorporated herein by reference in its entirety. The methods disclosed herein are compatible with a variety of 3′-based transcript counting methods (e.g., Rhapsody™ assay (Becton, Dickinson and Company (Franklin Lakes, N.J.)), Chromium™ Single Cell 3′ Solution (10X Genomics (San Francisco, Calif.))).

Whole Transcriptome Analysis (WTA)

FIG. 4 is a schematic illustration of a non-limiting exemplary workflow of performing whole transcriptome analysis using adapter ligation and random priming. A barcode 408 (e.g., a stochastic barcode) can be attached (e.g., conjugated, covalently attached, non-covalently attached) to a solid support 407 (e.g., a bead). The barcode 408 can comprise a target binding region (e.g., a poly(dT) tail 403 t) that can bind to RNA transcripts (e.g., poly-adenylated RNA transcripts 401) via a poly(dA) tail 402 r, or other nucleic acid targets, for labeling or barcoding (e.g., unique labeling). A reverse transcription reaction 400 a can be performed on the solid support. Excess barcodes attached to the solid support can be removed (e.g., by washing, by magnets). The reverse transcription reaction 400 a can produce a first strand labelled cDNA 409 attached to a solid support 407 (a bead illustrated here). The first strand labelled cDNA 409 can comprise cDNA 402 c 1 (the reverse complementary sequence of RNA sequence 402 r) and can comprise a poly(dT) sequence 403 t. The first strand labelled cDNA 409 can also comprise a number of labels, such as a unique molecular index (UMI) 404, a cellular label (CL) 405, and a universal PCR handle (Univ) 406 a (which can include, or be, for example, a binding site for a sequencing library amplification primer, such as the Read 1 sequencing primer). The universal PCR handle can comprise a first universal primer, a complimentary sequence thereof, a reverse complementary sequence thereof, a partial sequence thereof, or a combination thereof.

The first strand labelled cDNA 409 can undergo second strand synthesis 400 b thereby generating a double-stranded labeled cDNA molecule 410. Second strand synthesis can be performed by contacting the labelled cDNA molecule-mRNA hybrid with a nicking enzyme (e.g., RNaseH) that can nick the mRNA 401 hybridized to the labelled cDNA molecule 409, thereby generating nicked mRNA. The nicked mRNA can be used as a primer and extended using a polymerase (e.g., DNA Pol I), thereby incorporating the sequence of the first strand. The polymerase can comprise 5′-3′ exonuclease activity. The polymerase can degrade the downstream mRNA nicks that serve as the primers for the second strand synthesis. A ligase can be used to ligate the extended sequences together, thereby generating a second strand (e.g., double-stranded labeled cDNA molecule 410 comprising antisense cDNA). The double-stranded labeled cDNA molecule 410 can be end-polished and A-tailed at the free end to prepare for adapter ligation 400 c. The double-stranded labeled cDNA molecule 410 can be contacted with an adapter 421. The adaptor 421 can be single stranded, partially double-stranded, or fully double-stranded. The adaptor 421 can comprise a 5′ overhang which can comprise a first or second universal primer sequence. The adaptor 421 can comprise a free 5′ phosphate (P) which can ligate to the 3′ hydroxyl of the double-stranded labeled cDNA molecule 410. The adaptor 421 can ligate to both strands of the double-stranded labeled cDNA molecule 410, thereby producing adapter-ligated double-stranded labeled cDNA molecule 420.

In some embodiments, the adaptor comprises the sequence of a first universal primer, a complimentary sequence thereof, a partial sequence thereof, or a combination thereof. In some embodiments, the first universal primer comprises an amplification primer, a complimentary sequence thereof, a partial sequence thereof, or a combination thereof. In some embodiments, the first universal primer comprises a sequencing primer, a complimentary sequence thereof, a partial sequence thereof, or a combination thereof. In some embodiments, the sequencing primer comprises an Illumina sequencing primer. In some embodiments, the sequencing primer comprises a portion of an Illumina sequencing primer. In some embodiments, the sequencing primer comprises a P7 sequencing primer. In some embodiments, the sequencing primer comprises a portion of P7 sequencing primer. In some embodiments, the sequencing primer comprises a sequencing library amplification primer. In some embodiments, the sequencing primer comprises a Read 1 sequencing primer. In some embodiments, the sequencing primer comprises a Read 2 sequencing primer. In some embodiments, the adaptor comprises the sequence of a second universal primer, a complimentary sequence thereof, a partial sequence thereof, or a combination thereof. In some embodiments, the first universal sequence and the second universal sequence are the same. In some embodiments, the first universal sequence and the second universal sequence are different.

The term “adaptor” can refer to a single stranded, partially double-stranded, or double-stranded, oligonucleotide of at least 2, 5, 10, 15, 20 or 25 bases that can be attached to the end of a nucleic acid. Adaptor sequences can comprise, for example, priming sites, the complement of a priming site, and recognition sites for endonucleases, common sequences and promoters. The adaptor can be entirely or substantially double stranded. A double stranded adaptor can comprise two oligonucleotides that are at least partially complementary. The adaptor can be phosphorylated or unphosphorylated on one or both strands. The adaptor can have a double-stranded section and a single-stranded overhang section that is completely or partially complementary to an overhang (e.g., generated by a restriction enzyme, or a polymerase enzyme). In some embodiments the adaptor is a sequencing adaptor. In some embodiments, the adaptor is a single-stranded polynucleotide. In some embodiments, the adaptor is a double-stranded polynucleotide. In some embodiments, the adaptor, or a strand of the adaptor, is from 2-30 nucleotides in length. In some embodiments, the adaptor (or a strand of the adaptor) can comprise a nucleic acid sequence of at least 2 nucleotides, for example at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides, including ranges between any two of the listed values, for example 2-50, 2-45, 2-40, 2-35, 2-30, 2-25, 2-20, 2-15, 2-14, 2-13, 2-12, 2-11, 2-10, 2-9, 2-8, 2-7, 2-6, 6-50, 6-45, 6-40, 6-35, 6-30, 6-25, 6-20, 6-15, 6-14, 6-13, 6-12, 6-11, 6-10, 6-9, 6-8, 6-7, 7-50, 7-45, 7-40, 7-35, 7-30, 7-25, 7-20, 7-15, 7-14, 7-13, 7-12, 7-11, 7-10, 7-9, 7-8, 8-50, 8-45, 8-40, 8-35, 8-30, 8-25, 8-20, 8-15, 8-14, 8-13, 8-12, 8-11, 8-10, 8-9, 9-50, 9-45, 9-40, 9-35, 9-30, 9-25, 9-20, 9-15, 9-14, 9-13, 9-12, 9-11, 9-10, 10-50, 10-45, 10-40, 10-35, 10-30, 10-25, 10-20, 10-15, 10-14, 10-13, 10-12, or 10-11 nucleotides. The adaptor (or a strand of the adaptor) can comprise a nucleic acid sequence of at least 2 nucleotides of the sequence of a first universal primer, an amplification primer, a sequencing primer, a complimentary sequence thereof, a partial sequence thereof, or a combination thereof, for example at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides, including ranges between any two of the listed values, for example 2-50, 2-45, 2-40, 2-35, 2-30, 2-25, 2-20, 2-15, 2-14, 2-13, 2-12, 2-11, 2-10, 2-9, 2-8, 2-7, 2-6, 6-50, 6-45, 6-40, 6-35, 6-30, 6-25, 6-20, 6-15, 6-14, 6-13, 6-12, 6-11, 6-10, 6-9, 6-8, 6-7, 7-50, 7-45, 7-40, 7-35, 7-30, 7-25, 7-20, 7-15, 7-14, 7-13, 7-12, 7-11, 7-10, 7-9, 7-8, 8-50, 8-45, 8-40, 8-35, 8-30, 8-25, 8-20, 8-15, 8-14, 8-13, 8-12, 8-11, 8-10, 8-9, 9-50, 9-45, 9-40, 9-35, 9-30, 9-25, 9-20, 9-15, 9-14, 9-13, 9-12, 9-11, 9-10, 10-50, 10-45, 10-40, 10-35, 10-30, 10-25, 10-20, 10-15, 10-14, 10-13, 10-12, or 10-11 nucleotides of the sequence of a first universal primer, an amplification primer, a sequencing primer, a complimentary sequence thereof, a partial sequence thereof, or a combination thereof.

The adapter-ligated double-stranded labeled cDNA molecule 420 can be denatured 400 d, resulting in the adapter-ligated labeled cDNA molecule 430 that can serve as a template for full length cDNA amplification using primers 431 and 432 which anneal to first and/or second universal primer sequences in the universal PCR handle and adapter, thereby producing amplified adapter-ligated labeled cDNA molecule 440. This product 440 can be contacted with random primers 442 (e.g., degenerate primers of, of about, of at least, or of at most, 4, 5, 6, 7, 8, 9, 10, or more nucleotides in length) and undergo random priming 440 f. The random primers 442 can comprise an overhang comprising a universal PCR handle (Univ) 406 b (e.g., a binding site for a sequencing library amplification primer, such as the Read 2 sequencing primer). The random primers 442 can bind to different locations along the coding sequence of all transcripts and extend to generate an extension product 450 (e.g., a linearly amplified product). The extension product 450 comprises cDNA 402 c 2, which is of varying length depending on the binding site of random primer 442. The extension product 450 can be amplified with sequencing library amplification primers 451 and 452. Library amplification 400 g can add sequencing adapters 461 and 462 (e.g., P5 and P7 sequence) and sample index 463 (e.g., i5, i7) via overhangs in library forward primer 451 and library reverse primer 452. Library amplicons 460 can be sequenced and subjected to downstream methods of the disclosure. Paired-end sequencing using to generate 150 bp×2 sequencing reads can reveal the cell label, unique molecular index, poly(A) tail, and/or gene (or a partial sequence of the gene) on read 1, the gene (or a partial sequence of the gene and/or poly(A) tail on read 2, and the sample index on index 1 read. Methods, compositions, systems, devices, and kits for whole transcriptome amplification using stochastic barcodes have been previously disclosed, for example, in U.S. Pat. Pub. No. 2016/0312276, the content of which is hereby expressly incorporated by reference in its entirety.

In some embodiments, each one of the plurality of amplicons comprises at least part of the first universal sequence, the second universal sequence, or both. In some embodiments, the first universal sequence and the second universal sequence are the same. In some embodiments, the first universal sequence and the second universal sequence are different.

Expression Profiling with Target-Specific Multiplex Primer Panel

FIG. 5 is a schematic illustration of a non-limiting exemplary workflow of determining the expression profile of a panel of target genes using a panel of target-specific multiplex primers. A barcode 508 (e.g., a stochastic barcode) can be attached (e.g., conjugated, covalently attached, non-covalently attached) to a solid support 507 (a bead illustrated here). The barcode can comprise a target binding region (e.g., a poly(dT) tail 502 t) that can bind to RNA molecules (e.g., poly-adenylated mRNA transcripts 503 via a poly(dA) tail 502 a), or other nucleic acid targets, for labeling or barcoding (e.g., unique labeling). The barcode can comprise a number of labels, such as a unique molecular index (UMI) 504, a cellular label (CL) 505, and a universal PCR handle (Univ) 506 a (which can include, or be, for example, a binding site for a sequencing library amplification primer, such as the Read 1 sequencing primer). The universal PCR handle can comprise a first universal primer, a complimentary sequence thereof, a partial sequence thereof, or a combination thereof. A reverse transcription reaction 500 a can be performed on the solid support. Excess barcodes attached to the solid support can be removed (e.g., by washing, by magnets). The reverse transcription reaction can produce a first strand labelled cDNA 510. The first strand labelled cDNA 510 can comprise cDNA 501 c 1 (the reverse complement of mRNA sequence 501). First strand labelled cDNA 510 can undergo a first round of multiplex amplification (“Multiplex PCR 1 500 b”) employing a panel target-specific PCR 1 reverse primers 512 and a universal oligo forward primer 511 comprising a universal primer sequence (or a complement thereof). Multiplex PCR 1 500 b can comprise 1-30 cycles (e.g., 15 cycles). Multiplex PCR 1 amplicons 520 can comprise cDNA 501 c 2 (the length of which depends on the binding site of PCR 1 reverse primers 512 within the cDNA 501 c 1). Multiplex PCR 1 amplicons 520 can undergo a second of multiplex amplification (“Multiplex PCR 2 500 c”) employing a panel of nested target-specific PCR 2 reverse primers 521 and a universal oligo forward primer 511 comprising a universal primer sequence (or a complement thereof). The nested target-specific PCR 2 reverse primers 521 can include overhangs, which can include, or be, for example, a universal PCR handle (Univ) 506 b. Multiplex PCR 2 500 c can comprise 1-30 cycles (e.g., 8 cycles). Multiplex PCR 2 amplicons 530 can comprise cDNA 501 c 3 (the length of which depends on the binding site of PCR 2 reverse primers 521 within the cDNA 501 c 2). Multiplex PCR 2 amplicons 530 can undergo a third round of amplification (library amplification 500 c) with sequencing library amplification primers 531 and 532. Library amplification 500 d can add sequencing adapters 541 and 542 (e.g., P5 and P7 sequence) and sample index 543 (e.g., i5, i7) via overhangs in library forward primer 531 and library reverse primer 532. Library amplicons 540 can be sequenced and subjected to downstream methods of the disclosure. Sequencing using 150 bp×2 sequencing can reveal the cell label, unique molecular index, poly(A) tail, and/or gene (or a partial sequence of the gene) on read 1, the gene (or a partial sequence of the gene) and/or poly(A) tail on read 2, and the sample index on index 1 read.

Hybrid cDNA Library Preparation and/or Hybrid Sequencing Library Preparation

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides each comprising a first universal sequence and a barcode sequence; extending the plurality of oligonucleotides hybridized to the plurality of nucleic acid targets to generate a plurality of first strand barcoded polynucleotides; and synthesizing a plurality of second strand barcoded polynucleotides using the plurality of first strand barcoded polynucleotides as templates to generate a plurality of double-stranded barcoded polynucleotides. The method can comprise ligating an adaptor to the plurality of double-stranded barcoded polynucleotides, wherein the adaptor comprises a second universal sequence. The method can comprise amplifying the plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the second universal sequence, thereby generating a first plurality of barcoded amplicons comprising sequences of the nucleic acid targets, the first universal sequence, the second universal sequence, complements thereof, and/or a portion thereof, and primers capable of hybridizing to two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof.

In some embodiments, the method comprises performing random priming and extension using random primers and the first plurality of barcoded amplicons and/or the second plurality of barcoded amplicons, or products thereof, as templates. In some embodiments, performing random priming and extension comprises adding sequences of sequencing adaptors to the first plurality of barcoded amplicons and the second plurality of barcoded amplicons, or products thereof. In some embodiments, the method comprises performing extension and/or amplification using target-specific primers and the first plurality of barcoded amplicons and/or the second plurality of barcoded amplicons, or products thereof, as templates. In some embodiments, performing extension and/or amplification comprises adding sequences of sequencing adaptors to the first plurality of barcoded amplicons and/or the second plurality of barcoded amplicons. In some embodiments, said sequencing adaptors comprises a sequencing primer binding site or a sequencing primer. In some embodiments, the sequencing primer is a Read 1 sequencing primer or a Read 2 sequencing primer.

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides each comprising a first universal sequence and a barcode sequence; extending the plurality of oligonucleotides hybridized to the plurality of nucleic acid targets to generate a plurality of first strand barcoded polynucleotides; and synthesizing a plurality of second strand barcoded polynucleotides using the plurality of first strand barcoded polynucleotides as templates to generate a plurality of double-stranded barcoded polynucleotides. The method can comprise ligating an adaptor to the plurality of double-stranded barcoded polynucleotides, wherein the adaptor comprises a second universal sequence. The method can comprise amplifying the plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the second universal sequence, thereby generating a first plurality of barcoded amplicons comprising sequences of the nucleic acid targets, the first universal sequence, the second universal sequence, complements thereof, and/or a portion thereof. The method can comprise performing extension and/or amplification using target-specific primers and the first plurality of barcoded amplicons, or products thereof, as templates.

In some embodiments, the method comprises performing random priming and extension using random primers and the first plurality of barcoded amplicons, or products thereof, as templates. In some embodiments, the method comprises amplifying the plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof. In some embodiments, performing random priming and extension comprises adding sequences of sequencing adaptors to the first plurality of barcoded amplicons and the second plurality of barcoded amplicons, or products thereof. In some embodiments, performing extension and/or amplification comprises adding sequences of sequencing adaptors to the first plurality of barcoded amplicons. In some embodiments, said sequencing adaptors comprises a sequencing primer binding site or a sequencing primer. In some embodiments, the sequencing primer is a Read 1 sequencing primer or a Read 2 sequencing primer.

In some embodiments, amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets comprises amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of amplifying the first universal sequence. In some embodiments, amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets comprises amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of amplifying the second universal sequence. In some embodiments, extending the plurality of oligonucleotides to generate the plurality of first strand barcoded polynucleotides comprises performing reverse transcription of one or more nucleic acid targets of the plurality of nucleic acid targets.

In some embodiments, the plurality of first strand barcoded polynucleotides comprises barcoded single-stranded cDNA molecules. In some embodiments, the plurality of first strand barcoded polynucleotides comprises barcoded sequence of antibody oligonucleotide molecules. In some embodiments, extending the plurality of oligonucleotides to generate the plurality of first strand barcoded polynucleotides comprises performing nucleic acid extension of one or more oligonucleotides of the plurality of oligonucleotides hybridized to one or more nucleic acid targets of the plurality of nucleic acid targets. In some embodiments, the adaptor is ligated to 5′ end of the plurality of double-stranded barcoded polynucleotides. In some embodiments, the adaptor is ligated to 3′ end of the plurality of double-stranded barcoded polynucleotides. In some embodiments, the adaptor is ligated to both the 5′ end and 3′ end of the plurality of double-stranded barcoded polynucleotides. In some embodiments, the method comprises obtaining sequence information of the first plurality of the barcoded amplicons and/or the second plurality of the barcoded amplicons, or products thereof. In some embodiments, each one of the plurality of amplicons comprises at least part of the binding site for a sequencing library amplification primer (e.g., a Read 1 sequencing primer, a Read 2 sequencing primer)

In some embodiments, the two or more of the plurality of nucleic acid targets comprises mRNAs of a low-expressing gene. The two or more of the plurality of nucleic acid targets can comprise low abundant species. The two or more of the plurality of nucleic acid targets can comprise intermediate abundant species. The two or more of the plurality of nucleic acid targets can comprise low abundant species and intermediate abundant species. In some embodiments, the first plurality of barcoded amplicons comprises whole transcriptome amplification (WTA) products.

The first plurality of barcoded amplicons can correspond to at least 10% of the mRNAs of a single cell. The first plurality of barcoded amplicons can correspond to at least 50% of the mRNAs of a single cell. The first plurality of barcoded amplicons can correspond to at least 90% of the mRNAs of a single cell. In some embodiments, the first plurality of barcoded amplicons can correspond to at least 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%, or a number or a range between any two of these values, of the mRNAs of a single cell.

The second plurality of barcoded amplicons can correspond to at most 5% of the mRNAs of a single cell. The second plurality of barcoded amplicons can correspond to at most 10% of the mRNAs of a single cell. The second plurality of barcoded amplicons can correspond to at most 20% of the mRNAs of a single cell. In some embodiments, the second plurality of barcoded amplicons can correspond to at most 0.000000001%, 0.00000001%, 0.0000001%, 0.000001%, 0.00001%, 0.0001%, 0.001%, 0.01%, 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, or a number or a range between any two of these values, of the mRNAs of a single cell.

Adding the sequence of the adaptor to the plurality of double-stranded barcoded polynucleotides comprises ligating the adaptor to the plurality of double-stranded barcoded polynucleotides. Adding the sequence of the adaptor to the plurality of double-stranded barcoded polynucleotides can comprise adding the sequence of the adaptor to the plurality of double-stranded barcoded polynucleotides by nucleic acid extension or amplification, such as such as 1-100 cycles of extension or amplification. The amplification comprises polymerase chain reaction. Amplifying the plurality of double-stranded barcoded polynucleotides can comprise amplifying the plurality of double-stranded barcoded polynucleotides via linear amplification. The linear amplification can comprise 1-100 cycles of amplification, for example. Amplifying the plurality of double-stranded barcoded polynucleotides can comprise amplifying the plurality of double-stranded barcoded polynucleotides via polymerase chain reaction (PCR). The PCR can comprise 1-100 cycles of PCR. In some embodiments, performing the extension and/or amplification performing random performing 1-100 cycles of extension and/or amplification. The amplification can comprise polymerase chain reaction (PCR) amplification. In some embodiments, performing random priming, and extension and/or amplification, comprises performing 1-100 cycles of random priming, and extension and/or amplification. The amplification can comprise polymerase chain reaction (PCR) amplification.

The number of cycles of extension (e.g., nucleic acid extension) and/or amplification (e.g., linear amplification, PCR amplification) can be different in different implementations. In some embodiments, the amplification can comprise, or comprise about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 60, 70, 80, 90, 100, or a number or a range between any two of these values, cycles of amplification. In some embodiments, the linear amplification can comprise at least, or at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 60, 70, 80, 90, or 100, cycles of amplification.

Hybrid cDNA Library Preparation Followed by Non-Hybrid Sequencing Library Preparation

FIG. 6 is a schematic illustration of a non-limiting exemplary workflow of performing hybrid cDNA library preparation followed by non-hybrid sequencing library preparation. A barcode 608 (e.g., a stochastic barcode) can comprise a target binding region (e.g., a poly(dT) tail 606 t) that can bind to RNA transcripts (e.g., poly-adenylated RNA transcripts 602) via a poly(dA) tail 606 a, or other nucleic acid targets, for labeling or barcoding (e.g., unique labeling). The barcode 608 can be attached (e.g., conjugated, covalently attached, non-covalently attached) to a solid support (e.g., a bead). The reverse transcription reaction 600 a can produce a first strand labelled cDNA 616. The first strand labelled cDNA 616 can comprise cDNA 604 c 1 (the reverse complementary sequence of RNA sequence 604 r) and can comprise a poly(dT) sequence 606 t. The first strand labelled cDNA 616 can also comprise a number of labels, such as a unique molecular index (UMI) 614, a cellular label (CL) 612, and a universal PCR handle (Univ) 610 a (which can include, or be, for example, a binding site for a sequencing library amplification primer, such as the Read 1 sequencing primer). The universal PCR handle can comprise a first universal primer, a complimentary sequence thereof, a reverse complementary sequence thereof, a partial sequence thereof, or a combination thereof.

The first strand labelled cDNA 616 can undergo second strand synthesis 600 b thereby generating a double-stranded labeled cDNA molecule 618. Second strand synthesis can be performed by contacting the labelled cDNA molecule-mRNA hybrid with a nicking enzyme (e.g., RNaseH) that can nick the mRNA 602 hybridized to the labelled cDNA molecule 616, thereby generating nicked mRNA. The nicked mRNA can be used as a primer and extended using a polymerase (e.g., DNA Pol I), thereby incorporating the sequence of the first strand. The polymerase can comprise 5′-3′ exonuclease activity. The polymerase can degrade the downstream mRNA nicks that serve as the primers for the second strand synthesis. A ligase can be used to ligate the extended sequences together, thereby generating a second strand (e.g., double-stranded labeled cDNA molecule 618 comprising antisense cDNA). The double-stranded labeled cDNA molecule 618 can be end-polished and A-tailed at the free end to prepare for adapter ligation 600 c. The double-stranded labeled cDNA molecule 618 can be contacted with an adapter 622. The adaptor 622 can be single stranded, partially double-stranded, or fully double-stranded. The adaptor 622 can comprise a 5′ overhang which can comprise a first or second universal primer sequence. The adaptor 622 can comprise a free 5′ phosphate (P) which can ligate to the 3′ hydroxyl of the double-stranded labeled cDNA molecule 618. The adaptor 622 can ligate to both strands of the double-stranded labeled cDNA molecule 618, thereby producing adapter-ligated double-stranded labeled cDNA molecule 620.

The adapter-ligated double-stranded labeled cDNA molecule 620 can serve as a template for cDNA amplification 600 d. Amplification 600 d can comprise 1-30 cycles (e.g., 18-20 cycles). The cDNA amplification 600 d can comprise full length cDNA amplification and target-specific (e.g., gene-specific) cDNA amplification. The adapter-ligated double-stranded labeled cDNA molecule 620 can serve as a template for full length cDNA amplification using primers 624 and 626 n which anneal to first and/or second universal primer sequences in the universal PCR handle and adapter (and therefore are non-target-specific), thereby producing amplified adapter-ligated labeled cDNA molecule 630 w. The amplified adapter-ligated labeled cDNA molecule 630 w can comprise WTA cDNA sequence 604 c 2 w. The adapter-ligated double-stranded labeled cDNA molecule 620 can serve as a template for a target-specific (e.g., gene-specific) primer 626 s (e.g., a target-specific multiplex primer panel) and primer 624, thereby producing amplified adapter-ligated labeled cDNA molecule 630 s. Primer 626 s can be specific for a low expressing gene. The amplified adapter-ligated labeled cDNA molecule 630 s can comprise gene-specific cDNA sequence 604 c 2 s.

The cDNA amplicons 630 w and 630 s can be denatured 600 e, resulting in adapter-ligated labeled cDNA molecule 634. Adapter-ligated labeled cDNA molecule 634 can be contacted with random primers 636 (e.g., degenerate primers of, of about, of at least, or of at most, 4, 5, 6, 7, 8, 9, 10, or more nucleotides in length) and undergo random primer extension 600 f. The random primers 636 can comprise an overhang comprising a universal PCR handle (Univ) 610 b (e.g., a binding site for a sequencing library amplification primer, such as the Read 2 sequencing primer). The random primers 636 can bind to different locations along the coding sequence of all transcripts and extend to generate an extension product 638 (e.g., a linearly amplified product). The extension product 638 comprises cDNA 604 c 3, which is of varying length depending on the binding site of random primer 600 f. The extension product 638 can be amplified with sequencing library amplification primers 640 and 642. Size selection followed by library amplification 600 g can add sequencing adapters 646 and 648 (e.g., P5 and P7 sequence) and sample index 650 (e.g., i5, i7) via overhangs in library forward primer 640 and library reverse primer 642. Library amplicons 644 can be sequenced and subjected to downstream methods of the disclosure. Paired-end sequencing using to generate 150 bp×2 sequencing reads can reveal the cell label, unique molecular index, poly(A) tail, and/or gene (or a partial sequence of the gene) on read 1, the gene (or a partial sequence of the gene and/or poly(A) tail on read 2, and the sample index on index 1 read.

In some embodiments, each one of the plurality of amplicons comprises at least part of the first universal sequence, the second universal sequence, or both. In some embodiments, the first universal sequence and the second universal sequence are the same. In some embodiments, the first universal sequence and the second universal sequence are different.

Non-Hybrid cDNA Library Preparation Followed by Hybrid Sequencing Library Preparation

[0220] FIG. 7 is a schematic illustration of a non-limiting exemplary workflow of performing non-hybrid cDNA library preparation followed by hybrid sequencing library preparation. A barcode 708 (e.g., a stochastic barcode) can comprise a target binding region (e.g., a poly(dT) tail 706 t) that can bind to RNA transcripts (e.g., poly-adenylated RNA transcripts 702) via a poly(dA) tail 706 a, or other nucleic acid targets, for labeling or barcoding (e.g., unique labeling). The barcode 708 can be attached (e.g., conjugated, covalently attached, non-covalently attached) to a solid support (e.g., a bead). The reverse transcription reaction 700 a can produce a first strand labelled cDNA 716. The first strand labelled cDNA 716 can comprise cDNA 704 c 1 (the reverse complementary sequence of RNA sequence 704 r) and can comprise a poly(dT) sequence 706 t. The first strand labelled cDNA 716 can also comprise a number of labels, such as a unique molecular index (UMI) 714, a cellular label (CL) 712, and a universal PCR handle (Univ) 710 a (which can include, or be, for example, a binding site for a sequencing library amplification primer, such as the Read 1 sequencing primer). The universal PCR handle can comprise a first universal primer, a complimentary sequence thereof, a reverse complementary sequence thereof, a partial sequence thereof, or a combination thereof.

The first strand labelled cDNA 716 can undergo second strand synthesis 700 b thereby generating a double-stranded labeled cDNA molecule 718. Second strand synthesis can be performed by contacting the labelled cDNA molecule-mRNA hybrid with a nicking enzyme (e.g., RNaseH) that can nick the mRNA 702 hybridized to the labelled cDNA molecule 716, thereby generating nicked mRNA. The nicked mRNA can be used as a primer and extended using a polymerase (e.g., DNA Pol I), thereby incorporating the sequence of the first strand. The polymerase can comprise 5′-3′ exonuclease activity. The polymerase can degrade the downstream mRNA nicks that serve as the primers for the second strand synthesis. A ligase can be used to ligate the extended sequences together, thereby generating a second strand (e.g., double-stranded labeled cDNA molecule 718 comprising antisense cDNA). The double-stranded labeled cDNA molecule 718 can be end-polished and A-tailed at the free end to prepare for adapter ligation 700 c. The double-stranded labeled cDNA molecule 718 can be contacted with an adapter 722. The adaptor 722 can be single stranded, partially double-stranded, or fully double-stranded. The adaptor 722 can comprise a 5′ overhang which can comprise a first or second universal primer sequence. The adaptor 722 can comprise a free 5′ phosphate (P) which can ligate to the 3′ hydroxyl of the double-stranded labeled cDNA molecule 718. The adaptor 722 can ligate to both strands of the double-stranded labeled cDNA molecule 718, thereby producing adapter-ligated double-stranded labeled cDNA molecule 720.

The adapter-ligated double-stranded labeled cDNA molecule 720 can serve as a template for full length cDNA amplification 700 d (e.g., WTA). Amplification 700 d can comprise 1-30 cycles (e.g., 18-20 cycles). The adapter-ligated double-stranded labeled cDNA molecule 720 can serve as a template for full length cDNA amplification using primers 724 and 726 which anneal to first and/or second universal primer sequences in the universal PCR handle and adapter, thereby producing amplified adapter-ligated labeled cDNA molecule 730 (“WTA Product”). The amplified adapter-ligated labeled WTA Product 730 can comprise WTA cDNA sequence 704 c 2.

WTA Product 730 can be denatured 700 e and can be contacted with random primers 736 r (e.g., degenerate primers of, of about, of at least, or of at most, 4, 5, 6, 7, 8, 9, 10, or more nucleotides in length) and target-specific (e.g., gene-specific) primers 736 s and undergo random and gene-specific primer extension 700 f (e.g., hybrid library sequencing preparation). The random primers 736 r can comprise an overhang comprising a universal PCR handle (Univ) 710 b (e.g., a binding site for a sequencing library amplification primer, such as the Read 2 sequencing primer). The random primers 736 r can bind to different locations along the coding sequence of all transcripts and extend to generate an extension product (e.g., a linearly amplified product). The extension product 738 of random and gene-specific primer extension 700 f comprises cDNA 704 c 3, which is of varying length depending on the binding site of primers 736 r and 736 s. The extension product 738 can be amplified with sequencing library amplification primers 740 and 742. Size selection followed by library amplification 700 g can add sequencing adapters 746 and 748 (e.g., P5 and P7 sequence) and sample index 750 (e.g., i5, i7) via overhangs in library forward primer 740 and library reverse primer 742. Library amplicons 744 can be sequenced and subjected to downstream methods of the disclosure. Paired-end sequencing using to generate 150 bp×2 sequencing reads can reveal the cell label, unique molecular index, poly(A) tail, and/or gene (or a partial sequence of the gene) on read 1, the gene (or a partial sequence of the gene and/or poly(A) tail on read 2, and the sample index on index 1 read. In some embodiments, each one of the plurality of amplicons comprises at least part of the first universal sequence, the second universal sequence, or both. In some embodiments, the first universal sequence and the second universal sequence are the same. In some embodiments, the first universal sequence and the second universal sequence are different.

Combinations of Hybrid/Non-Hybrid cDNA Library Preparation and Hybrid/Non-Hybrid Sequencing Library Preparation

FIG. 8 is a schematic illustration of a non-limiting exemplary workflows for alternative single-cell RNA sequencing (scRNA-seq) embodiments comprising the methods of hybrid cDNA library preparation and/or hybrid sequencing library preparation as disclosed herein. In some embodiments, the workflow of performing whole transcriptome analysis (depicted in FIG. 4) comprises: (1) cell capture and barcoding of transcripts using an oligonucleotide comprising a single-stranded sequence of deoxythymine (dT) and a unique molecular indexes (UMI) 800 a; (2) PCR adapter ligation 800 b; (3) whole transcriptome amplification using ligated PCR adapter and 3′ adapter 800 c; and (4) random priming and PCR to attach Illumina sequencing adapters 800 e. In some embodiments, the workflow of determining the expression profile of a panel of target genes using a panel of target-specific multiplex primers (depicted in FIG. 5) comprises: (1) cell capture and barcoding of transcripts using an oligonucleotide comprising a single-stranded sequence of deoxythymine (dT) and a unique molecular indexes (UMI) 800 a; (2) PCR adapter ligation 800 b; (3) cDNA library preparation with a multiplex target-specific primer panel 800 d; and (4) random priming and PCR to attach Illumina sequencing adapters 800 e. In some embodiments, the workflow for performing the methods disclosed herein comprising hybrid cDNA library preparation followed by non-hybrid sequencing library preparation comprises: (1) cell capture and barcoding of transcripts using an oligonucleotide comprising a single-stranded sequence of deoxythymine (dT) and a unique molecular indexes (UMI) 800 a; (2) PCR adapter ligation 800 b; (3) whole transcriptome amplification using ligated PCR adapter and 3′ adapter 800 c and cDNA library preparation with a multiplex target-specific primer panel 800 d; and (4) random priming and PCR to attach Illumina sequencing adapters 800 e. In some embodiments, the workflow for performing the methods disclosed herein comprising non-hybrid cDNA library preparation followed by hybrid sequencing library preparation comprises: (1) cell capture and barcoding of transcripts using an oligonucleotide comprising a single-stranded sequence of deoxythymine (dT) and a unique molecular indexes (UMI) 800 a; (2) PCR adapter ligation 800 b; (3) whole transcriptome amplification using ligated PCR adapter and 3′ adapter 800 c; and (4) amplification with gene-specific specific primers 800 f and random priming and PCR to attach Illumina sequencing adapters 800 e. In some embodiments, the workflow for performing the methods disclosed herein comprising hybrid cDNA library preparation followed by hybrid sequencing library preparation comprises: (1) cell capture and barcoding of transcripts using an oligonucleotide comprising a single-stranded sequence of deoxythymine (dT) and a unique molecular indexes (UMI) 800 a; (2) PCR adapter ligation 800 b; (3) whole transcriptome amplification using ligated PCR adapter and 3′ adapter 800 c and cDNA library preparation with a multiplex target-specific primer panel 800 d; and (4) amplification with gene-specific specific primers 800 f and random priming and PCR to attach Illumina sequencing adapters 800 e.

Alternative Method of Hybrid cDNA Library Preparation Followed by Non-Hybrid Sequencing Library Preparation

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides; and extending the plurality of oligonucleotides in the presence of a reverse transcriptase and a template switch oligonucleotide (TSO) to generate a plurality of first strand barcoded polynucleotides comprising the TSO or a portion thereof, wherein each of the plurality of oligonucleotides and/or the template switch oligonucleotide comprises a first universal sequence and a barcode sequence. The method can comprise amplifying the plurality of first strand barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the TSO or the portion thereof to synthesize a first plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets, the first universal sequence, the TSO, or a portion thereof, and to generate a first plurality of double-stranded barcoded polynucleotides; and primers capable of hybridizing to two or more of the plurality of nucleic acid targets to synthesize a second plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets or a portion thereof, to generate a second plurality of double-stranded barcoded polynucleotides. The method can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and a sequence, or a subsequence, of the TSO, thereby generating a plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof. The method can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof.

Disclosed herein include methods of labeling nucleic acid targets. In some embodiments, the method comprises: hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides; and extending the plurality of oligonucleotides in the presence of a reverse transcriptase and a template switch oligonucleotide (TSO) to generate a plurality of first strand barcoded polynucleotides comprising the TSO or a portion thereof, wherein each of the plurality of oligonucleotides and/or the template switch oligonucleotide comprises a first universal sequence and a barcode sequence. The method can comprise amplifying the plurality of first strand barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and the TSO or the portion thereof to synthesize a first plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets, the first universal sequence, the TSO, or a portion thereof, and to generate a first plurality of double-stranded barcoded polynucleotides. The method can comprise amplifying the first plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and a sequence, or a subsequence, of the TSO, thereby generating a first plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof, and primers capable of hybridizing to the two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof.

In some embodiments, the method comprises amplifying the plurality of first strand barcoded polynucleotides using primers capable of hybridizing to two or more of the plurality of nucleic acid targets to synthesize a second plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets or a portion thereof, to generate a second plurality of double-stranded barcoded polynucleotides. In some embodiments, each of the plurality of oligonucleotides comprises a first universal sequence and a barcode sequence. In some embodiments, the template switch oligonucleotide comprises a second universal sequence. In some embodiments, the template switch oligonucleotide comprises a first universal sequence and a barcode sequence. In some embodiments, each of the plurality of oligonucleotides comprises a second universal sequence. In some embodiments, the method comprises amplifying the first plurality of second strand barcoded polynucleotides and/or the second plurality of second strand barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets. In some embodiments, amplifying the first plurality of second strand barcoded polynucleotides and/or the second plurality of second strand barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of hybridizing to the first universal sequence. In some embodiments, amplifying the first plurality of second strand barcoded polynucleotides and/or the second plurality second strand barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of hybridizing to the sequence, or a subsequence, of the TSO.

In some embodiments, amplifying the plurality of first stranded barcoded polynucleotides comprises amplifying the plurality of first stranded barcoded polynucleotides comprises 1-100 cycles. Amplifying the plurality of first stranded barcoded polynucleotides can comprise performing linear amplification of the plurality of first stranded barcoded polynucleotides. Amplifying the plurality of first stranded barcoded polynucleotides can comprise performing polymerase chain reaction (PCR) amplification of the plurality of first stranded barcoded polynucleotides. Amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides 1-100 cycles. Amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides via linear amplification. Amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides can comprise amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides via polymerase chain reaction (PCR) amplification.

The number of cycles of extension (e.g., nucleic acid extension) and/or amplification (e.g., linear amplification, PCR amplification) can be different in different implementations. In some embodiments, the amplification can comprise, or comprise about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 60, 70, 80, 90, 100, or a number or a range between any two of these values, cycles of amplification. In some embodiments, the linear amplification can comprise at least, or at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 60, 70, 80, 90, or 100, cycles of amplification.

In some embodiments, the method comprises performing extension and/or amplification using target-specific primer and the first plurality of barcoded amplicons and/or second plurality of barcoded amplicons as templates. In some embodiments, extending the plurality of oligonucleotides to generate the plurality of first strand barcoded polynucleotides comprises performing reverse transcription of one or more nucleic acid targets of the plurality of nucleic acid targets to generate barcoded single-stranded cDNA molecules. In some embodiments, extending the plurality of oligonucleotides to generate the plurality of first strand barcoded polynucleotides comprises performing nucleic acid extension of oligonucleotides of the plurality of oligonucleotides hybridized to one or more nucleic acid targets of the plurality of nucleic acid targets to generate barcoded single-stranded nucleic acid molecules.

FIG. 9 is a schematic illustration of a non-limiting exemplary workflow of performing hybrid cDNA library preparation followed by hybrid sequencing library preparation. A barcode 908 (e.g., a stochastic barcode) can comprise a target binding region (e.g., a poly(dT)VN 907) that can bind to RNA transcripts (e.g., poly-adenylated RNA transcripts 902) via a poly(dA) tail 906 a, or other nucleic acid targets, for labeling or barcoding (e.g., unique labeling). In some embodiments, the 3′ end of the poly(dT)VN sequence 907 comprises at least one “VN” doublet (in which each “V” is any of A, C, or G, and in which “N” is any of A, G, C, or T). In some embodiments the barcode is associated with a solid support (e.g., a bead). In some embodiments, the bead can be a polymeric bead, for example a deformable bead or a gel bead, functionalized with barcodes or stochastic barcodes (such as gel beads from 10X Genomics (San Francisco, Calif.). In some implementation, a gel bead can comprise a polymer-based gels. Gel beads can be generated, for example, by encapsulating one or more polymeric precursors into droplets. Upon exposure of the polymeric precursors to an accelerator (e.g., tetramethylethylenediamine (TEMED)), a gel bead may be generated.

The reverse transcription reaction 900 a can be performed. During reverse transcription 900 a, upon reaching the 5′ end of the barcode 908, the terminal transferase activity of an enzyme (e.g., a reverse transcriptase, such as a Moloney murine leukemia virus (MMLV)) adds a few additional nucleotides (e.g., deoxycytidine, CCC 916) to the 3′ end of the newly synthesized cDNA sequence strand 904 c 1 (the reverse complementary sequence of RNA sequence 904 r). These CCC bases 916 can function as an anchoring site of the template switch oligonucleotide 918, which comprises a sequence complementary to the tailed sequence (e.g., rGrGrG 920). The template switch oligonucleotide 918 can comprise at least part of the first universal sequence, the second universal sequence, or both. Upon base pairing between the rGrGrG 920 and the appended deoxycytidine stretch 916, the enzyme “switches” template strands, from barcode 908 to the template switch oligonucleotide 918, and continues replication to the 5′ end of the template switch oligonucleotide 918. Thus, the resulting first strand labelled cDNA 923 contains a reverse complement sequence of the template switch oligonucleotide 918. The first strand labelled cDNA 923 can comprise cDNA 904 c 1 (the reverse complementary sequence of RNA sequence 904 r). The first strand labelled cDNA 923 can also comprise a number of labels, such as a unique molecular index (UMI) 914, a partition label (PL) 912, and Read 1 sequence 910 a.

The term “template switching” can refer to the ability of a reverse transcriptase to switch from an initial nucleic acid sequence template to the 3′ end of a new nucleic acid sequence template having little or no complementarity to the 3′ end of the nucleic acid synthesized from the initial template. An example of template switching is the ability of a reverse transcriptase to switch from an initial nucleic acid sequence template/primer substrate to the 3′ end of a new nucleic acid sequence template having little or no complementary to the 3′ end of the nucleic acid primer strand. Template switching allows, e.g., a DNA copy to be prepared using a reverse transcriptase that switches from an initial nucleic acid sequence template to the 3′ end of a new nucleic acid sequence template having little or no complementarity to the 3′ end of the DNA synthesized from the initial template, thereby allowing the synthesis of a continuous product DNA that directly links an adaptor sequence to a target oligonucleotide sequence without ligation. Template switching can comprise ligation of adaptor, homopolymer tailing (e.g., polyadenylation), random primer, or an oligonucleotide that the polymerase can associate with. In any of the above-mentioned embodiments, template switching may be used to introduce a universal adaptor sequence or the complement thereof.

The first strand labelled cDNA 923 comprising a reverse complement sequence of the template switch oligonucleotide 918 can undergo a reaction to generate a template for amplification 900 b (e.g., denaturing), resulting in the labeled cDNA molecule 923. The labeled cDNA molecule 923 can serve as a template for cDNA amplification 900 c. Amplification 900 c can comprise 1-30 cycles (e.g., 18-20 cycles). The cDNA amplification 900 c can comprise full length cDNA amplification and target-specific (e.g., gene-specific) cDNA amplification. The labeled cDNA molecule 923 can serve as a template for full length cDNA amplification using primers 924 and 926 n which anneal to Read 1 910 a and sequences in the template switch oligonucleotide 918 (and therefore are non-target-specific), thereby producing amplified labeled cDNA molecule 930 w. The amplified labeled cDNA molecule 930 w can comprise cDNA sequence 904 c 2 w. The labeled cDNA molecule 923 can serve as a template for a target-specific (e.g., gene-specific) primer 926 g (e.g., a target-specific multiplex primer panel) and primer 924, thereby producing amplified labeled cDNA molecule 930 s. Primer 926 g can be specific for a low expressing gene. The amplified labeled cDNA molecule 930 s can comprise gene-specific cDNA sequence 904 c 2 s.

The amplified labeled cDNA molecule 930 s and the amplified labeled cDNA molecule 930 w can undergo process 900 d, comprising enzymatic fragmentation, end repair, A-tailing, adaptor ligation (comprising Read 2 910 b) and sample index PCR (adding library index 938, and P5 936, and P7 940 via sample index primer overhangs) thereby producing library amplicon 934. Library amplicons 934 can be sequenced and subjected to downstream methods of the disclosure. Paired-end sequencing using to generate 150 bp×2 sequencing reads can reveal the cell label, unique molecular index, poly(A) tail, and/or gene (or a partial sequence of the gene) on read 1, the gene (or a partial sequence of the gene and/or poly(A) tail on read 2, and the sample index on index 1 read.

In some embodiments, the reverse transcriptase comprises a viral reverse transcriptase. In some embodiments, the viral reverse transcriptase is a murine leukemia virus (MMLV) reverse transcriptase. In some embodiments, the viral reverse transcriptase is a Moloney murine leukemia virus (MMLV) reverse transcriptase.

The cDNA library amplification products can be fragmented to produce a plurality of nucleic acid fragments. For example, the cDNA library amplification products can be fragmented for adaptor ligation. Fragmentation can include fragmentation using a mechanical (Covaris focused electroacoustic, Nebulizer, sonication, vortex,) or enzymatic (e.g. Fragmentase) fragmentation. Alternatively, the labeled amplicons may be fragmented by to Tagmentation (Nextera). In some embodiments, the fragmentation can be partial fragmentation, so that fragments of the target nucleic acid molecule can have different lengths. Fragmentation can be conducted by, for example, sonication, restriction enzyme digestion, or any other suitable methods. In some embodiments, two or more of the plurality of nucleic acid fragments have the same 5′ terminus but different 3′ terminus. In some embodiments, each of the plurality of nucleic acid fragments has a length between 50 nt to 10,000 nt. In some embodiments, the plurality of nucleic acid fragments comprises at least 2 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 10 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 100 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 1,000 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 10,000 nucleic acid fragments. In some embodiments, the fragmenting comprises restriction digestion of the first plurality of amplicons. In some embodiments, at least 50% of the plurality of nucleic acid fragments comprises different length. In some embodiments, at least 80% of the plurality of nucleic acid fragments comprises different length. In some embodiments, at least 90% of the plurality of nucleic acid fragments comprises different length. In some embodiments, the fragments can be subject to purification (e.g., washing) to remove fragments that do not comprise the molecular barcode.

Methods for ligating adaptors to fragments of nucleic acid are well known. Adaptors may be double-stranded, single-stranded or partially single-stranded. In some aspects, adaptors are formed from two oligonucleotides that have a region of complementarity, for example, about 10 to 30, or about 15 to 40 bases of perfect complementarity; so that when the two oligonucleotides are hybridized together they form a double stranded region. Optionally, either or both of the oligonucleotides may have a region that is not complementary to the other oligonucleotide and forms a single stranded overhang at one or both ends of the adaptor. Single-stranded overhangs may be about 1 to about 8 bases, or about 2 to about 4. The overhang may be complementary to the overhang created by cleavage with a restriction enzyme to facilitate “sticky-end” ligation. Adaptors may include other features, such as primer binding sites and restriction sites.

Non-Hybrid cDNA Library Preparation Followed by Split Pools of Hybrid and Non-Hybrid Sequencing Library Preparation

In some embodiments, the method comprises dividing the first plurality of second strand barcoded polynucleotides and the second plurality of second strand barcoded polynucleotides into two pools, wherein amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides using (1) the primers capable of hybridizing to the first universal sequence and a sequence, or a subsequence, of the TSO is performed in one reaction using one of the two pools and (2) the primers capable of hybridizing to two or more of the plurality of nucleic acid targets is performed in another reaction using the other of the two pools. In some embodiments, the method comprises obtaining sequence information of the first plurality of barcoded amplicons and/or second plurality of barcoded amplicons, or products thereof. In some embodiments, the amplicons of the first and second pools are re-pooled prior to obtaining sequence information.

In some embodiments, the method comprises dividing the first plurality of second strand barcoded polynucleotides and the second plurality of second strand barcoded polynucleotides into three pools, wherein the third pool comprises another panel of target-specific primers (distinct from the primers in the first two pools) for amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides. In some embodiments, the method comprises dividing the first plurality of second strand barcoded polynucleotides and the second plurality of second strand barcoded polynucleotides into four or more pools, wherein each pool comprises a distinct set of primers for amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides.

The number of cycles of amplification (e.g., linear amplification, PCR amplification) of the first plurality of second strand barcoded polynucleotides and/or the second plurality of second strand barcoded polynucleotides of the two (or more pools) can be different in different implementations. In some embodiments, the amplification can comprise, or comprise about, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 60, 70, 80, 90, 100, or a number or a range between any two of these values, cycles of amplification. In some embodiments, the amplification can comprise at least, or at most, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 60, 70, 80, 90, or 100, cycles of amplification.

FIG. 10 is a schematic illustration of a non-limiting exemplary workflow of performing non-hybrid cDNA library preparation followed by splitting the amplicons into pools for separately conducing non-hybrid sequencing library preparation and hybrid sequencing library preparation. A barcode 1008 (e.g., a stochastic barcode) can comprise a target binding region (e.g., a poly(dT)VN 1007) that can bind to RNA transcripts (e.g., poly-adenylated RNA transcripts 1002) via a poly(dA) tail 1006 a, or other nucleic acid targets, for labeling or barcoding (e.g., unique labeling). In some embodiments, the 3′ end of the poly(dT)VN sequence 1007 comprises at least one “VN” doublet (in which each “V” is any of A, C, or G, and in which “N” is any of A, G, C, or T). In some embodiments the barcode is associated with a solid support (e.g., a bead). In some embodiments, the bead can be a polymeric bead, for example a deformable bead or a gel bead, functionalized with barcodes or stochastic barcodes (such as gel beads from 10X Genomics (San Francisco, Calif.). In some implementation, a gel bead can comprise a polymer based gels. Gel beads can be generated, for example, by encapsulating one or more polymeric precursors into droplets. Upon exposure of the polymeric precursors to an accelerator (e.g., tetramethylethylenediamine (TEMED)), a gel bead may be generated.

The reverse transcription reaction 1000 a can be performed. During reverse transcription 1000 a, upon reaching the 5′ end of the barcode 1008, the terminal transferase activity of an enzyme (e.g., a reverse transcriptase, such as a Moloney murine leukemia virus (MMLV)) adds a few additional nucleotides (e.g., deoxycytidine, CCC 1016) to the 3′ end of the newly synthesized cDNA sequence strand 1004 c 1 (the reverse complementary sequence of RNA sequence 1004 r). These CCC bases 1016 can function as an anchoring site of the template switch oligonucleotide 1018, which comprises a sequence complementary to the tailed sequence (e.g., rGrGrG 1020). The template switch oligonucleotide 1018 can comprise at least part of the first universal sequence, the second universal sequence, or both. Upon base pairing between the rGrGrG 1020 and the appended deoxycytidine stretch 1016, the enzyme “switches” template strands, from barcode 1008 to the template switch oligonucleotide 1018, and continues replication to the 5′ end of the template switch oligonucleotide 1018. Thus, the resulting first strand labelled cDNA 1023 contains a reverse complement sequence of the template switch oligonucleotide 1018. The first strand labelled cDNA 1023 can comprise cDNA 1004 c 1 (the reverse complementary sequence of RNA sequence 1004 r). The first strand labelled cDNA 1023 can also comprise a number of labels, such as a unique molecular index (UMI) 1014, a partition label (PL) 1012, and Read 1 sequence 1010 a.

The first strand labelled cDNA 1023 comprising a reverse complement sequence of the template switch oligonucleotide 1018 can undergo a reaction to generate a template for amplification 1000 b (e.g., denaturing), resulting in the labeled cDNA molecule 1023. The labeled cDNA molecule 1023 can serve as a template for cDNA amplification 1000 c. Amplification 1000 c can comprise 1-30 cycles (e.g., 18-20 cycles). The labeled cDNA molecule 1023 can serve as a template for full length cDNA amplification using primers 1024 and 1026 which anneal to Read 1 1010 a and sequences in the template switch oligonucleotide 1018 (and therefore are non-target-specific), thereby producing amplified labeled cDNA molecule 1030. The amplified labeled cDNA molecule 1030 can comprise cDNA sequence 1004 c 2.

The plurality of amplified labeled cDNA molecule 1030 can be split into two pools. In one pool, the amplified labeled cDNA molecule 1030 can undergo PCR 1000 d 2 with target-specific (e.g., gene-specific) primers (which add Read 2 1010 b, library index 1038, and P5 1036, and P7 1040 via primer overhangs) thereby producing library amplicon 1034 s. In another pool, the amplified labeled cDNA molecule 1030 undergo process 1000 d 1, comprising enzymatic fragmentation, end repair, A-tailing, adaptor ligation (comprising Read 2 1010 b) and sample index PCR (adding library index 1038, and P5 1036, and P7 1040 via sample index primer overhangs) thereby producing library amplicon 1034 w. Library amplicons 1034 w and 1034 s can be sequenced and subjected to downstream methods of the disclosure.

Terminology

In at least some of the previously described embodiments, one or more elements used in an embodiment can interchangeably be used in another embodiment unless such a replacement is not technically feasible. It will be appreciated by those skilled in the art that various other omissions, additions and modifications may be made to the methods and structures described above without departing from the scope of the claimed subject matter. All such modifications and changes are intended to fall within the scope of the subject matter, as defined by the appended claims.

One skilled in the art will appreciate that, for this and other processes and methods disclosed herein, the functions performed in the processes and methods can be implemented in differing order. Furthermore, the outlined steps and operations are only provided as examples, and some of the steps and operations can be optional, combined into fewer steps and operations, or expanded into additional steps and operations without detracting from the essence of the disclosed embodiments.

With respect to the use of substantially any plural and/or singular terms herein, those having skill in the art can translate from the plural to the singular and/or from the singular to the plural as is appropriate to the context and/or application. The various singular/plural permutations may be expressly set forth herein for sake of clarity. As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural references unless the context clearly dictates otherwise. Any reference to “or” herein is intended to encompass “and/or” unless otherwise stated.

It will be understood by those within the art that, in general, terms used herein, and especially in the appended claims (e.g., bodies of the appended claims) are generally intended as “open” terms (e.g., the term “including” should be interpreted as “including but not limited to,” the term “having” should be interpreted as “having at least,” the term “includes” should be interpreted as “includes but is not limited to,” etc.). It will be further understood by those within the art that if a specific number of an introduced claim recitation is intended, such an intent will be explicitly recited in the claim, and in the absence of such recitation no such intent is present. For example, as an aid to understanding, the following appended claims may contain usage of the introductory phrases “at least one” and “one or more” to introduce claim recitations. However, the use of such phrases should not be construed to imply that the introduction of a claim recitation by the indefinite articles “a” or “an” limits any particular claim containing such introduced claim recitation to embodiments containing only one such recitation, even when the same claim includes the introductory phrases “one or more” or “at least one” and indefinite articles such as “a” or “an” (e.g., “a” and/or “an” should be interpreted to mean “at least one” or “one or more”); the same holds true for the use of definite articles used to introduce claim recitations. In addition, even if a specific number of an introduced claim recitation is explicitly recited, those skilled in the art will recognize that such recitation should be interpreted to mean at least the recited number (e.g., the bare recitation of “two recitations,” without other modifiers, means at least two recitations, or two or more recitations). Furthermore, in those instances where a convention analogous to “at least one of A, B, and C, etc.” is used, in general such a construction is intended in the sense one having skill in the art would understand the convention (e.g., “a system having at least one of A, B, and C” would include but not be limited to systems that have A alone, B alone, C alone, A and B together, A and C together, B and C together, and/or A, B, and C together, etc.). In those instances where a convention analogous to “at least one of A, B, or C, etc.” is used, in general such a construction is intended in the sense one having skill in the art would understand the convention (e.g., “a system having at least one of A, B, or C” would include but not be limited to systems that have A alone, B alone, C alone, A and B together, A and C together, B and C together, and/or A, B, and C together, etc.). It will be further understood by those within the art that virtually any disjunctive word and/or phrase presenting two or more alternative terms, whether in the description, claims, or drawings, should be understood to contemplate the possibilities of including one of the terms, either of the terms, or both terms. For example, the phrase “A or B” will be understood to include the possibilities of “A” or “B” or “A and B.”

In addition, where features or aspects of the disclosure are described in terms of Markush groups, those skilled in the art will recognize that the disclosure is also thereby described in terms of any individual member or subgroup of members of the Markush group.

As will be understood by one skilled in the art, for any and all purposes, such as in terms of providing a written description, all ranges disclosed herein also encompass any and all possible sub-ranges and combinations of sub-ranges thereof. Any listed range can be easily recognized as sufficiently describing and enabling the same range being broken down into at least equal halves, thirds, quarters, fifths, tenths, etc. As a non-limiting example, each range discussed herein can be readily broken down into a lower third, middle third and upper third, etc. As will also be understood by one skilled in the art all language such as “up to,” “at least,” “greater than,” “less than,” and the like include the number recited and refer to ranges which can be subsequently broken down into sub-ranges as discussed above. Finally, as will be understood by one skilled in the art, a range includes each individual member. Thus, for example, a group having 1-3 articles refers to groups having 1, 2, or 3 articles. Similarly, a group having 1-5 articles refers to groups having 1, 2, 3, 4, or 5 articles, and so forth.

From the foregoing, it will be appreciated that various embodiments of the present disclosure have been described herein for purposes of illustration, and that various modifications may be made without departing from the scope and spirit of the present disclosure. Accordingly, the various embodiments disclosed herein are not intended to be limiting, with the true scope and spirit being indicated by the following claims. 

What is claimed is:
 1. A method of labeling nucleic acid targets, comprising: (a) hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides each comprising a first universal sequence and a barcode sequence; (b) extending the plurality of oligonucleotides hybridized to the plurality of nucleic acid targets to generate a plurality of first strand barcoded polynucleotides; (c) synthesizing a plurality of second strand barcoded polynucleotides using the plurality of first strand barcoded polynucleotides as templates to generate a plurality of double-stranded barcoded polynucleotides; (d) adding the sequence of an adaptor to the plurality of double-stranded barcoded polynucleotides, wherein the adaptor comprises a second universal sequence; and (e) amplifying the plurality of double-stranded barcoded polynucleotides using: (e1) primers capable of hybridizing to the first universal sequence and the second universal sequence, thereby generating a first plurality of barcoded amplicons comprising sequences of the nucleic acid targets, the first universal sequence, the second universal sequence, a complementary sequence thereof, and/or a portion thereof, and (e2) primers capable of hybridizing to two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, the first universal sequence, the second universal sequence, a complementary sequence thereof, and/or a portion thereof.
 2. The method of claim 1, comprising (f1) performing extension and/or amplification using target-specific primers and the first plurality of barcoded amplicons and/or the second plurality of barcoded amplicons, or products thereof, as templates.
 3. The method of claim 2, wherein (f1) performing extension and/or amplification comprises adding sequences of binding sites of sequencing primers and/or sequencing adaptors, complementary sequences thereof, and/or portions thereof, to the first plurality of barcoded amplicons and/or the second plurality of barcoded amplicons.
 4. The method of claim 1, comprising (f2) performing random priming, and extension and/or amplification, using random primers and the first plurality of barcoded amplicons and/or the second plurality of barcoded amplicons, or products thereof, as templates.
 5. The method of claim 4, wherein (f2) performing random priming, and extension and/or amplification, comprises adding sequences of binding sites of sequencing primers and/or sequencing adaptors, complimentary sequences thereof, and/or portions thereof, to the first plurality of barcoded amplicons and the second plurality of barcoded amplicons, or products thereof.
 6. The method of claim 1, wherein (e2) amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets comprises amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of amplifying the first universal sequence.
 7. The method of claim 1, wherein (e2) amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets comprises amplifying the plurality of double-stranded barcoded polynucleotides using the primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of amplifying the second universal sequence.
 8. The method of claim 1, wherein the sequence of the adaptor is added to 5′ end, 3′ end, or both of the plurality of double-stranded barcoded polynucleotides.
 9. The method of claim 1, comprising (g) obtaining sequence information of the first plurality of the barcoded amplicons and/or the second plurality of the barcoded amplicons, or products thereof.
 10. The method of claim 1, wherein the first plurality of barcoded amplicons are correspond to at least 10%, at least 50%, or at least 90%, of the mRNAs of a single cell.
 11. The method of claim 1, wherein the second plurality of barcoded amplicons correspond to at most 5%, at most 10%, or at most 20%, of the mRNAs of a single cell.
 12. The method of claim 1, wherein the adaptor is a single-stranded polynucleotide.
 13. The method of claim 1, wherein the adaptor is a double-stranded polynucleotide.
 14. The method of claim 1, wherein each one of the plurality of amplicons comprises at least part of the first universal sequence, the second universal sequence, or both.
 15. The method of claim 1, wherein (d) adding the sequence of the adaptor to the plurality of double-stranded barcoded polynucleotides comprises ligating the adaptor to the plurality of double-stranded barcoded polynucleotides.
 16. The method of claim 1, wherein (d) adding the sequence of the adaptor to the plurality of double-stranded barcoded polynucleotides comprises adding the sequence of the adaptor to the plurality of double-stranded barcoded polynucleotides by nucleic acid extension or amplification.
 17. The method of claim 1, wherein the two or more of the plurality of nucleic acid targets comprises mRNAs of a low-expressing gene.
 18. The method of claim 1, wherein the total number of occurrences of the two or more of the plurality of nucleic acid targets comprises mRNAs of at most 1%, at most 5%, or at most 10%, of the mRNAs of a single cell.
 19. A method of labeling nucleic acid targets, comprising: (a) hybridizing a plurality of nucleic acid targets with a plurality of oligonucleotides; (b) extending the plurality of oligonucleotides in the presence of a reverse transcriptase and a template switch oligonucleotide (TSO) to generate a plurality of first strand barcoded polynucleotides comprising the TSO or a portion thereof, wherein each of the plurality of oligonucleotides and/or the template switch oligonucleotide comprises a first universal sequence and a barcode sequence; (c) amplifying the plurality of first strand barcoded polynucleotides using (c1) primers capable of hybridizing to the first universal sequence and the TSO or the portion thereof to synthesize a first plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets, the first universal sequence, the TSO, or a portion thereof, and to generate a first plurality of double-stranded barcoded polynucleotides; and (c2) primers capable of hybridizing to two or more of the plurality of nucleic acid targets to synthesize a second plurality of second strand barcoded polynucleotides each comprising a sequence of the nucleic acid targets or a portion thereof, to generate a second plurality of double-stranded barcoded polynucleotides; and (d1) amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the first universal sequence and a sequence, or a subsequence, of the TSO, thereby generating a first plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof.
 20. The method of claim 19, further comprising (d2) amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets, thereby generating a second plurality of barcoded amplicons comprising sequences of the two or more of the plurality of nucleic acid targets, and/or a portion thereof.
 21. The method of claim 19, wherein each of the plurality of oligonucleotides comprises a first universal sequence and a barcode sequence, and wherein the template switch oligonucleotide comprises a second universal sequence.
 22. The method of claim 19, wherein the template switch oligonucleotide comprises a first universal sequence and a barcode sequence, and wherein each of the plurality of oligonucleotides comprises a second universal sequence.
 23. The method of claim 19, wherein (c2) amplifying the plurality of first strand barcoded polynucleotides comprises amplifying the first plurality of second strand barcoded polynucleotides and/or the second plurality of second stranded barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of hybridizing to the first universal sequence.
 24. The method of claim 19, wherein (c2) amplifying the plurality of first strand barcoded polynucleotides comprises amplifying the first plurality of second strand barcoded polynucleotides and/or the second plurality of second stranded barcoded polynucleotides using primers capable of hybridizing to the two or more of the plurality of nucleic acid targets and a primer capable of hybridizing to the sequence, or a subsequence, of the TSO.
 25. The method of claim 19, comprising dividing the first plurality of second strand barcoded polynucleotides and/or the second plurality of second strand barcoded polynucleotides into two pools, wherein amplifying the first plurality of double-stranded barcoded polynucleotides and/or the second plurality of double-stranded barcoded polynucleotides using (d1) the primers capable of hybridizing to the first universal sequence and a sequence, or a subsequence, of the TSO is performed in one reaction using one of the two pools and (d2) the primers capable of hybridizing to two or more of the plurality of nucleic acid targets is performed in another reaction using the other of the two pools. 